فهرست مطالب سید نظام الدین حسینی

Recombinant human interleukin-1 receptor antagonist (IL-1RA) is a protein with 153 amino acids and molecular weight of 16.83 kDa. This drug protein is known as Anakinra, and has an effective application in the treatment of rheumatoid arthritis. This study was conducted to examine the produce of the recombinant IL-1RA protein in Escherichia coli (E. coli) strains.

Codon optimization of IL-1RA gene was done using GenScript, and the gene was cloned in the pUC18 as cloning vector. Then, plasmid was cut by two restriction enzymes including NdeI and BamH1 enzymes. IL-1RA gene was purified from the agarose gel. IL-1RA gene was ligated into expression vector. The constructed expression cassette was transformed into E. coli BL21 (DE3) Origami (DE3) and Rosetta (DE3) using CaCl2</sub> and heat shock method.

Identification and confirmation of transformed colonies was performed using colony polymerase chain reaction (PCR). Induction of this gene was done with isopropyl β- d-1-thiogalactopyranoside (IPTG). The protein expression was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting techniques, and it purified by Ni nickel resin. Expression analysis of transformed E. coli strains confirmed that gene integrated into expression host. Molecular weight of expressed protein was estimated to be 16.83 kDa.

 In this study, Human IL-1RA was successfully produced in E. coli Origami with high quantity other than the rest of E. coli strains. Therefore, E. coli BL21 Origami (DE3) can be used as the suitable host for production of recombinant IL-1RA, and this technology has a potential for localization.

Clinical diagnostic kits for detecting hepatitis B are based on antibodies, and have inefficient sensitivity, stability, and cost. Therefore, researches about aptamers and using them instead of antibodies may make these defects up. In this study, a biotinylated anti-hepatitis B virus surface antigen (anti-HBsAg) aptamer sequence was used to evaluate the possibility of specific detection of HBsAg via enzyme-linked aptamer assay (ELAA) method using biotin-streptavidin system.
HBsAg was immobilized in Maxibinding plate (SPL, Korea) by using carbonate-bicarbonate buffer (pH: 9.4), and by considering different variables. Immobilization of HBsAg was evaluated by commercial kit. Aptamer sequence was cloned and imbalance polymerase chain reaction (PCR) was improved and performed using plasmid as DNA template. Then, biotinylated aptamer was utilized in enzyme-linked aptamer assay method via taking advantage of streptavidin-biotin signal amplification system.
Findings: Antigen immobilization was set up using conjugates number 1 and 2 of commercial kit. Colony polymerase chain reaction confirmed aptamer cloning in Escherichia coli (E. coli) Top10 host. The best gradient polymerase chain reaction result was achieved at 64 ºC annealing temperature. Control group in assays showed accuracy of immobilization; but no repetitive specific signal was obtained that could prove joining of aptamer to HBsAg, and detection of that.
Three-dimensional structure of an immobilized antigen on a solid surface may vary from that which exists on the surface of viruses or cells. Negative results might be due to the ability of aptamers to attach into antigens with totally intact shape. Conjugation of aptamer with biotin may interfere in aptamer conformation; so, a connective arm between aptamer and biotin could be a suggestion, which needs more assessments.

Proper management of sewage is one of the most important environmental issues, organic matter and nutrients, including phosphorus compounds, are the potential pollutants of the receiving waters. Release of phosphorus from municipal wastewater treatment plant effluent to the environment is one of the main reasons for the phenomenon of the Eutrophication. Therefore, the aim of this study was to determine the amount of nutrients and organic matter in wastewater of Yasouj city and efficient removal of nutrients such as total phosphorus by modified Bardenpho system.
This is a cross-sectional study. The sampling method as Composite Sampling and study population was samples of treatment system input (after screening system) and output of treatment system (after sedimentation). In order to identify of affecting factors on phosphorus removal use of modified Bardenpho pilot. The amount of nitrate, total phosphate, COD and BOD5 removal for 9 months was evaluated. The collected data were analyzed by using SPSS software (version 16).
Findings: According to the results, the average of input COD in system (674.55±228.54), input phosphorous (21.26±4.8), input nitrate (25.91±19.63), input BOD (378/33±106/66) mg per liter and the input pH (7.22±0.35). There is a significant Relationship between the input COD and percentage of phosphorus removal (P.value=0/004), but there is not significant relationship between the pH input and phosphorus removal percentage (P.value=0.339). Most phosphorus removal was observed in Solids Retention Time (SRT) of 15 days (69.72%). Internal recycle of 200 percent (68/96%) showed the highest percentage of phosphorous removal.
Discussion and According to obtained information, the optimum conditions for phosphorus removal showed SRT =15, HRT =0/5-1 in anaerobic tank, the internal recycle percentage of 200%, recycled activated sludge (75%) and keep the DO =2-3. Therefore, for removal of phosphorus addition of physical and chemical methods can use of modified biological processes. In this systems, by replacing of an anaerobic stage at the beginning of the process, improve phosphorus removal. This method can be a good alternative to treatment plants with activated sludge system.