فهرست مطالب غلام رضا فاضلی

The purpose of this study was to investigate the enhanced thermal resistance mechanism of the DU145 tumor spheroid cultures as compared to the prostate carcinoma cell line's monolayer cultures. DU145 cells were cultured either as spheroids or monolayers. Cultures were treated with hyperthermia in a precision water bath (at 43°C for 60 minutes) and/or quercetin (50 and 500 μM for monolayer and spheroid cultures respectively). After hyperthermic treatment, the cell viability colony forming ability, and the expression of heat shock protein 70 (Hsp70) were examined in both culture systems. Hsp70 expression was studied using the western blot method. Our results showed that the DU145 monolayer and spheroid cell culture treatment with hyperthermia alone resulted in a marked survival inhibition. Furthermore, the spheroids showed a more significant resistance to hyperthermia compared to the monolayer cultures (p = 0.01). They also produced more Hsp70 than the monolayer cultures. Treatment of cells with quercetin reduced the Hsp70 level in both culture systems. However, with the reduced Hsp70 levels, thermal resistance of the spheroids showed a greater decrease in relation to that of the monolayers. The results suggest that the enhanced hyperthermia resistance mechanism of the spheroid cultures compared to that of the monolayer cultures can be attributed to spheroid's Hsp70 production.

Breast cancer is the most common cancer affecting women and: of the population will develop breast cancer before the age of 70. Of those, 5 to 0 % of these cases develop breast cancer due to autosomal dominant predisposing gene mutation at BRCA and BRCA. The aim of this study was to assess the prevalence of these mutations among breast cancer patients and their relatives in Ahwaz. For this purpose, 38 and 3 blood samples from breast cancer patients and their healthy relatives respectively were tested for possible presence of mutation in exon 3 of the BRCA gene using single strand conformational analysis method.the results showed that 0.5% of the patients with breast cancer carried the mutated gene.These results demonstrated that the prevalence of BRCA gene mutation in our area is similar to its global pattern.