غلامرضا نادری

Acanthamoeba is a resistant protozoan that causes severe diseases, such as GAE and CAK. Because many medications are ineffective on the parasite, the quest to find alternative drugs is in progress.

This research was aim13 ed to assess the performance of the black and green tea extracts on Acanthamoeba.

The clinical isolate of Acanthamoeba was cultured on non-nutrient agar plates. The black and green tea extracts were prepared via maceration, dried by rotary evaporation, and stored at 4 °C. The main component of the extracts (caffeine) was recognized using GC-MS analysis. The effects of three concentrations of black and green tea extracts were evaluated on the parasite and compared with the control and current drug.

Black and green tea extracts have lethal effects on Acanthamoeba cysts and the performance was more than that shown by the control and the current anti-keratitis drug. Moreover, the effectiveness of the tea extracts was tim 25 e- and dose-dependent (P < 0.0001). There was no significant differences between the performance of black and green tea (P > 0.05).

Black 13 and green tea extracts demonstrated the potential to inhibit the Acanthamoeba parasite, but the use of tea extract in clinical applications requires further study.

To determine the best planting pattern of rapeseed cv. Zarfam, an experiment was carried out during two years of 2010 and 2011 at Agricultural Research Station of Varamin, Iran. The experiment was in split-plot design based on complete randomized block with 12 treatments and four replications. The main plots included four planting methods including one or two rows on furrow with 60 or 50 cm spacing and the sub plots included three seed rates of 6, 8 and 10 kg/ha. Combined analysis showed that interaction between planting methods and seed rates was significant on day to germinate, day of ripening, and number of silique per plant and weight of 1000 seed. Planting method of one row on furrow with 60 cm spacing caused longest period of crop maturity, number of pod per plant, number of seed per silique, 1000 seed weight and oil percentage. Seed rate up to 8 kg had proper results and increasing the number of rows and reducing space between caused decrement in yield and yield components. The method of one row planting on furrow with 60 cm spacing had the most seed number in silique and oil percentage. Also, 60 cm spacing on one row and 8 kg/ha seed rate had suitable effect on most of the studied traits would be recommendable for rapessed cv. Zarfam in this region.

This survey was conducted to study the relationship between total length of Capoeta gracilis (with selection of two length ranges, including TL>11cm and TLC. gracilis were different in Taleghan River. The results also revealed that the length class with total length of more than 11 cm selects habitats with higher elevation (p