فهرست مطالب فروزنده فریدونی

Diverse prevalence of oral mucosal lesions has been reported among different populations. There are few researches about the prevalence of oral mucosal lesions in Iran. The aim of the current study was to investigate the prevalence of oral mucosal lesions and associated factors in pathology department of Tehran Cancer Institute of Imam Khomeini Hospital from 2000 to 2010. This research was conducted on existing data. The patients’ age, gender, site of the lesion, type of the lesion and microscopic diagnosis were recorded. Prevalence of lesions were evaluated with (CI=%95) all data were analyzed by x2 Test under spss (16). Oral mucosal lesions were present in 784 cases out of 40092 cases (1.96%). Epithelial lesions were found to be the most prevalent oral mucosal lesions of all (90%). The most common location reported for the lesions was the lips (26.8%).The mean age for the patients was 43±17 years of age and there was no association found between the type of the lesion and the sex of the subjects (p<0.09). Squamous Cell Carcinoma was the most prevalent mucosal lesion which is a malignant epithelial tumor and its early detection is essential.

Estrogen receptor alpha protein status is determined by routine immunohistochemistry analysis in all malignant breast tumors. This assay has its limitations. RNA based techniques are potential complements for immunohistochemistry but it must be noticed that gene silencing may occur at different levels from RNA to protein. The aim of this study was the comparison of the results from these two assays and characterizing the tumors subgroup in which gene expression occurs at RNA level but the target protein is absent. 92 primary breast tumors including their clinical and IHC results were collected before treatment. Estrogen receptor gene expression of tumors was studied by Reverse Transcription Polymerase Chain Reaction (RT PCR). In this assay, GAPDH was used as a reference gene. 36.6 % of tumors with negative estrogen receptor protein showed gene expression at mRNA level. In this subgroup most of the patient were older than 50 years and in stages 3 or 4 of breast cancer and had poor prognosis according to Nottingham prognostic index. Most cases of the perineural invasion have been seen in this subgroup. It seems that RT-PCR assay would enable us to recognize a subgroup of breast tumors with poor prognosis which expresses RNA but not protein.

Resistance to drugs with different structure and function is a great obstacle to the successful treatment of breast cancer. A well-known mechanism responsible for drug resistance is over-expression of ABC-transporter genes such as MDR1. MDR1 gene polymorphisms may result in drug resistance. In this study we investigated the possible correlation between MDR1 gene C3435T polymorphism and clinicopathologic findings and response to treatment in Iranian breast cancer patients. In this descriptive study we investigated C3435T polymorphism of MDR1 gene in 54 breast cancer patients admitted to Imam Khomeini Hospital of Tehran and 40 individuals without history of breast cancer by PCR-RFLP technique during 2006-2008. Association of this polymorphism with response to treatment and clinicopathologic characteristics were also investigated. Data were analyzed using Chi-Square test and SPSS software. P values<0.05 were considered to be statistically significant. In patient group frequencies of genotypes were 18% for CC, 56%for CT and %26 for TT whereas in control group frequencies of genotypes were %23 for CC, %50 for CT and %27 for TT. There was no significant difference in frequency of C3435T polymorphism between breast cancer patients and healthy controls. Frequency of C3435T polymorphism between responders and non-responders was not significant. It seems that there is no correlation between C3435T polymorphism of MDR1 gene and response to treatment, clinical findings and pathologic characteristics of breast cancer. So the role of C3435T polymorphism in response to treatment is still controversial.