لیلا آقایی دیزج

Origanum is a plant of the Lamiaceae family that is considered one of the most important and best-selling medicinal plants in the world. The aerial parts, especially the leaves of different species of Origanum, have always been used as one of the most popular spices and flavorings in the food, perfume and cosmetics industries. Drought is one of the most important abiotic stresses may cause deleterious damage to plants. Lack of access to adequate amount of water or increase in transpiration can induce drought stress in plants and alter the production of metabolites. Drought stress reduces the water and turgor potentials, thereby negatively affecting various physiological processes. Chitosan is a natural biodegradable substance derived from crustacean shells such as crabs and shrimp. Having unique biological and physiological properties, it has found several applications in various industries such as pharmaceuticals, medicine and agriculture. As a bio-elicitor, chitosan prevents severe damage to plants under stress conditions by increasing the plant's defense activity.

This experiment was performed in 2019 in the research greenhouse of Azarbaijan Shahid Madani University, Iran as a factorial experiment in the complete randomized block design with three replications. The experiment treatments included three different concentrations of chitosan as foliar application (0, 250, 500 mg/l), and drought stress treatment at two levels (no stress and water deficit stress) in two species of Origanum (Origanum vulgare, Origanum majorana). The Origanum seeds were obtained from the Forest and Rangeland Research Organization and planted inside the seedling trays. Then, as the plants reached the desired growth of 4- and 5-leaves stages, each of the plant seedlings was transferred to plastic pots with the opening diameter of 15 cm, height of 25 cm, and capacity of 4 kg. The soil transferred to the pots had a loamy texture and a mixture of 33.32% sand, 40% silt, and 26.48% clay. Also, the soil inside the pots had a pH equal to 7.96, electrical conductivity of 2.8, organic matter of 2.415%, organic carbon of 1.401%, and calcium carbonate of 12.5%. The pots were kept in the greenhouse for 16 hours in light at 24 °C and 8 hours in dark at 18 °C. The application of water deficit stress treatment and foliar application of chitosan was performed in three stages three weeks before the complete flowering. In this experiment, a TDR moisture meter was used to measure the soil moisture of each pot for applying the water deficit treatment.

The results of the present study show that the water deficit stress reduces total shoot dry weight. This reduces the yield when regarding the yield of aerial part of the plant. It should be noted in this study that the foliar application of chitosan under the water deficit conditions improved this trait. In this study, the foliar application of chitosan at the concentration of 500mg/l under the water deficit stress conditions increased the shoot dry weight. The results showed that the water deficit stress reduced relative water content, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids, while increasing the hydrogen peroxide (H2O2), malondialdehyde (MDA) and proline contents. The foliar application of chitosan under water deficit stress conditions increased RWC, chlorophyll a, carotenoids, total chlorophyll, and proline contents.

According to the results of this study, by conducting extensive research, the use of chitosan as a bio-elicitor to reduce the water deficit stress in the medicinal plant of Origanium can be suggested and the positive aspects of chitosan to be used for improving the physiological parameters in Origanium and other medicinal plants. In the end, it is suggested to perform this research in the areas with different climates, especially arid and semi-arid climates, with different concentrations of chitosan on different plants.