مرضیه مزرعه خطیری

Diabetes is one of the metabolic disorders that leads to various cardiovascular and metabolic diseases. The aim of this study was to investigate the interactive effect of high-intensity interval training (HIIT) and thyme honey on Pdx1 gene expression and insulin resistance index among type 2 diabetic male rats.

Totally, 36 rats became diabetic by intraperitoneal injection of strepotozocin after 20 weeks of high-fat diet. The rats were divided into four groups: control, HIIT, honey, HIIT+honey. Eight weeks of HIIT, five sessions per week was performed. Thyme honey at a dose of 3g/Kg/day was gavaged. Glucose, insulin, insulin resistance index, and Pdx1 gene expression were measured. Data were analyzed by one-way ANOVA and post hoc Bonferroni’s tests.

Our results showed that glucose and insulin resistance index decreased in the HIIT+honey group compared to the control group (p=0.001). There was a significant increase in insulin (p=0.001) and a significant decrease in Pdx1 gene expression (p=0.0001) in all experimental groups compared to the control group.

HIIT and thyme honey had an effect on reducing glucose and insulin resistance index. In addition, HIIT and honey reduced the expression of Pdx1 gene in pancreatic tissue.

The aim of this study was to investigate the interactive effect high interval exercise training and thyme honey extract on glucose and lipide profile of type 2 diabetic male rats.

The statistical population of the present study consisted of male rats.After 20 weeks of dieting with a high-fat diet and then intraperitoneally injected with a low dose of 25 mg of STZ per kilogram of weight. rats with fasting glucose between 150 and 400 mg / dL were considered type 2 diabetic rat.Diabetic rats divided In 4 groups of diabetic control (6 heads), HIIT (8), thyme honey (6), HIIT and thyme honey (8 heads), HIITand HIIT- thyme honey were performed Eight-week aerobic exercise program, five sessions per week in the 2-minute HIIT with 80 to 90 % Vo2max and a one-minute break with 50 to 56% vo2max. Thyme honey Gavage was administered orally with a 3 g / g 5 days a week during the protocol. At the end of the training period, the rats were anesthetized by Ether and sacrificed. Glucose and lipide profiles were measured using the autoanalyzer. Statistical analysis was performed using SPSS22 software and two-factor analysis of variance and effect size and Bonferoni post Hock test for determination to compare the effect of independent variables.

The research findings showed that the LDL, TG, CHOL of lipid profile in the HIIT group and thyme honey compared to diabetic control decreased, and TG significant decreased in the HIIT (P=0.01). Also, HDL in the group of HIIT and thyme honey and the interaction of HIIT-thyme honey compared to control had a significant increase.

Therefore, it was concluded that the interactive effect of HIIT and consumption of thyme honey has been effective in improving glucose and fat profile.