مینا صادقیان

Progress in nano-science and nanotechnology in the past decade has provided many opportunities to study the biological effects of nanoparticles, in particular their anti-bacterial effects. The aim of this study was to evaluate the antimicrobial properties of silver nanoparticles for the removal of Klebsiella pneumoniae isolated from industrial effluents.
In this experimental study, after sampling polluted industries, coliform and total coliform measurements of fecal industrial wastewater microbiology were performed by standard methods. The antimicrobial activity of silver nanoparticles on the bacteria (Klebsiella pneumoniae isolates and standard) was evaluated with the agar dilution method and broth dilution. One milliliter suspension containing bacteria at 1.5 × 108 CFU/mL was added to each sample followed by incubation at 37°C for 24 hours. After the mentioned period, the optical density at a wavelength of 600 nm was used to measure the concentration of bacteria. Next, 100 mL of each dilution was transferred to solid medium followed by incubation. The results were analyzed with SPSS 22 software.
Fecal and total coliform bacteria pollution of textile wastewater was approved, and Klebsiella pneumoniae (textile industry pollution index) were isolated. With increasing concentration, the antibacterial activity of silver nanoparticles increased while the number of colonies decreased. Although none of the concentrations were able to eliminate the bacteria, a non-significant decrease in the number of bacteria was observed.
The results of the study showed that the type of bacteria and concentrations of silver nanoparticles antimicrobial properties of silver nanoparticles are risk factors. Although the concentrations used were effective against bacteria yet they did not lead to complete elimination of bacteria Therefore potential impact of nanoparticles for use requires further research and economic factors and other factors should be considered in their application.

Today, research on new antimicrobial materials is growing and nanoparticles can serve as good representative.
The purpose of this study was to evaluate the antimicrobial properties of silver nanoparticles in the removal of bacteria E.Coli isolated from industrial effluents.
In this experimental study, after sampling polluted industries, coliform and total coliform measurements fecal industrial wastewater microbiology was performed by standard methods. The silver nanoparticles with different concentrations of the nutrient media broth dilution method in the bacterium E.Coli isolates and standard (PTCC8739) and the effect of turbidity tubes (OD), which represents growth of bacteria at a wavelength of 600 nm spectrophotometer was read. Then, all tubes were inoculated on nutrient agar solid medium. The results were analyzed using statistical methods and software SPSS22.
Total coliform and fecal coliform contamination was approved in slaughterhouse wastes and E.Coli (slaughterhouse industry pollution index). was isolated. With increasing concentrations, anti-bacterial activity of silver nanoparticles increased and the number of colonies was reduced, although none of the concentrations of bacteria was able to contain and eliminate the bacteria. The effect varies with type of bacteria, but this difference was not statistically significant.
The results showed that the concentration of bacteria and type of bacteria are effective on the range of antimicrobial activities of silver nanoparticles. On the other hand, although the concentrations used in the study of E.Coli were effective, but did not remove the whole bacteria and economic feasibility and other factors need to be considered in the application of nanoparticles.

Due to the unique absorption and antibacterial properties of nanoparticles, their use in water and wastewater treatment to remove bacteria is spreading. This research aimed to study the effect of silver nanoparticles in the removal of Enterococcus faecalis isolated from industrial sewage. after preliminary studies, field studies including sampling of industrial wastewater was conducted. First, Fecal Coliform, total coliform and Enterococcus faecalis of industrial Sewage were measured by standard methods of microbiology, then the effect of concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.81, and 3. 9 PPM of isolated nanoparticles serial dilution method on Enterococcus faecalis and standard (PTCC 1339) was determined. Next, and the turbidity of tube (OD), which represents growth of bacteria, was read at 600 nm with a spectrophotometer. After that, all tubes were cultured on solid medium and the results were analyzed using SPSS 22. fecal and total coliform contamination of industrial Sewage from slaughter and textile plant were approved and Enterococcus faecalis was isolated. The growth of isolates and standard bacteria was not controlled by any of these concentrations. None of the concentrations was able to remove the bacteria but the increase of concentration led to higher effect of nanoparticles, and in accordance with the type of bacteria, it was different, but the difference was not statistically significant. Although silver nanoparticles, according to the concentration, affect the Streptococcus faecalis, they cannot completely remove bacteria. Therefore, the economic factors and other involved factors need to be considered in the application of nanoparticles.