فهرست مطالب مینو ایلخانی پور

Stress has different adverse effects on the biological processes of living organisms. Therefore, replacing herbal medicines with fewer side effects in the treatment of diseases and improving health is the most important. In this study the effect of aqueous extracts of thyme on blood biochemical parameters and liver tissue in female rats under immobilization stress were studied. In present experimental study, 24 adult female Wistar rats in weight range of 173±12 g were randomly divided into four groups of six rats in each: group1, Control, group2, the group immobilized stress, groups 3and 4, groups to immobilized stress by oral prescription extract of thyme the doses of 100 and 400 mg/kg respectively. At the end of the studies, serum cholesterol, LDL and HDL and MDA, TAC in liver tissue homogenates were estimated. The results indicated significant reduction in the level of cholesterol, LDL, MDA in treated groups with aqueous extract of thyme and a significant increase in HDL, TAC. The extract of Thymus vulgaris L be due their antioxidant can partly reduction the serum level of cholesterol, LDL and liver tissue protection against the damaging effects of immobilization stress.

The aim of this study was to investigate the survival of Lactobacillus casei LAFTI-L26 in Dutch red cheese. For this purpose, the effect of thermal treatments (pasteurization and non-pasteurization) in different concentrations of brine (0%, 2.5%, and 5%) and the ripening time (1,30 and 60 days) on lactobacillus casei survival and physicochemical properties of cheese including; pH, dry matter, fat percentage, salt percentage and acidity were studied. The results from the beginning of production to the end of the 60-day period showed that Increasing the pH of cheeses increased significantly (p<0.05). During non-pasteurization, the fat content of pasteurized cheeses increased significantly (p<0.05), and in pasteurized cheeses it was much more than pasteurized cheeses. Total microbial load increased significantly during storage (p<0.05). The acidity of the cheeses decreased significantly during storage (p<0.05), and with increasing the duration of salting water in pasteurized cheeses significantly increased (p<0.05). The salt percentage of samples was significantly reduced by applying the thermal process (p<0.05). By increasing the storage time, the number of Lactobacillus casei bacteria increased significantly in all samples (p<0.05), with increasing percentage of Lactobacillus casei salt in pasteurized cheese increased significantly (p <0.05), and pasteurization Increased the viability of Lactobacillus casei. In general, Dutch red cheese produced in Salmas has the ability to carry Lactobacillus casei (LAFTI-L26 DSL).

Nowadays, herbal products often have been used as an alternative or supplement to chemical drugs in treat of diseases with low side effects and cost of treatment. The aim of this study was to survey the effect of aqueous extract of Cinnamon verum on anxiety-like behavior in streptozotocin-induced (STZ) diabetic rats.
This experimental study was carried out on 28 of male Wistar rats with the weight range of 200 ± 30 (g) in which randomly divided into four groups including seven each. The diabetes induced in adult male Wistar rats with an intraperitoneal injection of streptozotocin (50 mg/kg). The experiments were assigned to 3 weeks, rats were treated with extract in dose of 200mg/kg oral in which at the end of experiment animals were transferred to the elevated plus maze (EPM) at adjusted intervals and their anxiety reactions including enterance numbers and time spent in open arm were recorded and investigated in 5 min. The data were analyzed using SPSS software, method of One-way ANOVA and were compared different groups with each other by tukey test. Finally, P≤0/05 was considered statistically significant.
A significant decrease in entrance numbers and time spent in open arm of the elevated plus maze in groups of the treated diabetic rats were seen whereas; there were no significant changes in their anxiety reactions in other groups (non-diabetic)(P≤0.05).
It was thought in this survey, extract of Cinnamon verum with more than 50 different combinations and different effects, can affect nerve disorders of anxiety in adult male rats. But, the findings show that C. verum extract cannot effect on the anxiety-like behavior in adult male rats. However, the anxiety-like behavior increased in induced diabetic adult male rats, significantly. Therefore, it could be suggested that on the one hand probably the concentration of cinnamon used to treat diabetic rats was not sufficient to reduce blood glucose levels and improved effects did not have on the function of neurotransmitter systems for anxiety control. On the other hand, it is likely changes in the activity of neurotransmitter systems in the brain and the occurrence of anxiety behaviors in diabetic rats has been influenced by factors other than glucose levels. As a result, cinnamon extract had no effect on the improvement of anxiety and anxiety – like behaviors due to low blood glucose levels.

Oxidative stress causes damage in processes, such as learning and memory through affecting the nervous system. Also, using chemical drugs for treatment of stress-induced depression is often associated with attenuation of memory. In the present study, the effect of hydroalcoholic extract of Noroozak (Salvia leriifolia) root as an antioxidant along with antidepressant drug, fluvoxamine, were investigated on the spatial memory of male rats under immobilization stress.
In this experimental study, 25 adult male rats (mean weight, 170g), were randomly divided into five groups of 5 each. The animals were orally treated with different doses of drug and extract for 21 days. At the end of the treatment period, a radial 8-arm maze was used to investigate the behavioral indices. Also, malondialdehyde and catalase levels in the brain tissue homogenate and glucose and cortisol levels in blood, were measured. Data analysis was carried out using one-way analysis of variance and post-hoc Tukey’s test.
Spatial memory in the rats under immobilization stress (sham group) showed a significant decrease as compared to the control group. Administration of extract of Noroozak root improved the spatial memory in the groups receiving extract and extract-drug compared to sham and fluvoxamine groups. Also, the extract of Noroozak root improved the flovoxamine-induced attenuation of memory, increased catalase, and decreased malondialdehyde, glucose, and cortisol in the groups under treatment with extract and extract-drug compared to sham group.
The findings showed that immobilization stress and fluvoxamine administration for the treatment of stress-induced depression resulted in attenuation of spatial memory; while, the extract of Noroozak root improved memory in all groups due to powerful antioxidant property.

In the present study, tea shoots consist of an apical bud and two adjacent leaves, in two tea varieties (Hybrid and Colone 100), were collected from tea research center of country and after extraction, the content of total phenol, total flavonoids and antioxidant activity were evaluated by colorimetric method. Catechins and caffeine were analyzed by HPLC. Sensory properties evaluation was done by panel test using linear method. Results showed that total phenolic content in two types, Colone100 and Hybrid had not significant difference (p≤0.05). Flavonoid content and Antioxidant properties were different in two varieties and it was significantly (p≤0.05) higher in Colone 100 than Hybrid. HPLC result analysis showed higher levels of catechins and caffeine in Hybrid variety than Colone 100 variety. Considering colour and taste of the samples, Hybrid variety was stronger, vice versa Colone 100 had stronger aroma than Hybrid variety that expresses the superiority of Colone 100 in terms of its health properties to Hybrid variety.

Nigella Sativa with a rich medical and religious history, has useful chemical compounds that have beneficial effects on human health. In this research, the effect of alcoholic extract of Nigella sativa on blood parameters were investigated.
This experimental study was carried out on 28 mature female Wistar rats (weight, 160-200g) in 4 groups (7 mice/group). The first group (control group) received no drug, the second (N. Sativa) and third (N. Sativa詩ꡮ⥢춫抢 stress) groups received alcoholic extract of N. Sativa at a dose of 400mg/kg for 21 days through gavage. It should be noted that the third group underwent immobilization stress in addition to gavage treatment; the fourth group only received immobilization stress. Blood samples were taken from the heart ventricle and then blood factors were measured using Melet. Ms. Cell Counter device. The results were analyzed by one-way ANOVA and the level of significance was considered at p In the experimental group (N. Sativa䫚�), white blood cells and platelets percentage significantly increased, compared to the control and stress groups, respectively (p The results of this study showed that the effect of alcoholic extract of N. Sativa on blood factor increase immunity of the body and reduce and eliminate the negative effects of stress on the blood factors.

Hippocampus is the most important part of learning and memory in the brain. Melissa officinalis naturally affects the nervous system and induces calmness. Lorazepam is also used in the treatment of insomnia and anxiety. In this study, the effect of Lorazepam and aqueous extract of Melissa officinalis (AEMO) was investigated on hippocampus tissue and spatial memory in male rats using radial maze method.
In this experimental study, 24 male Wistar rats were randomly divided into four groups. Control group received normal diet and water, the second group received lorazepam (dose. 10mg/kg), and two other groups received AEMO (at doses of 10 and 100mg/kg) by gavage for 18 days. Then, their spatial memory were tested in an 8 arm radial maze (RAM). The level of lipid peroxidation of homogenized brain tissue was assessed, and hippocampal tissue sections were prepared and after H&E staining, DG area was investigated under a microscope. Data were analyzed using one-way ANOVA and Tukey statistical tests. The level of significance was set at p There was no significant difference in learning level between animals received lorazepam and control group. The results of the experiments showed the positive effect of low dose of AEMO (10mg/kg) on spatial memory, while high dose of Melissa officinalis (100mg/kg) prevented memory formation.
The results of this research showed that AEMO can increase short-term memory at low dose (10mg/kg), but it may prevent spatial memory formation at high doses.

Nigella sativa L. grain has a rich medical and religious history. Also, due to the importance of learning and memory, human being is always looking for ways to increase the ability of them. The aim of this study was to investigate the effect of alcoholic extract of Nigella Sativa L. on memory and learning.
The present study was carried out on 28 adult female Wistar rats (weighing 160-200 g) in 4 groups (7 mice per group). The first group (control group) received no treatment, the second and third groups were treated orally with alcoholic extract of Nigella sativa, at a dose of 400 mg/kg of body weight by gavage for 21 days. In addition to oral gavage treatment, the third group was also treated with immobilization stress, and the 4th group was only given immobilization stress to animals. Animals were trained for 3 days and on day 21, (without the extract intake) were conducted memory tests using radial arm 8 branches. On the 1st day and the 21th day of the experiment, all mice by digital scales were weighed. The data were analyzed by One-way ANOVA and Tukey’s post hoc test. The level of significance was considered at P According to the results of Nigella sativa group and "Nigella sativa Stress" group significantly enhanced spatial learning and memory compared to the control group and stress group, respectively (P The obtained results showed alcoholic extract of Nigella Sativa enhances learning and reduces the negative effects of stress on learning and spatial memory and also causes proportionable increase of bodyweight.

Diabetes is the most common metabolic disease in the world. Diabetic Complications, oxidative stress induced by free radicals linked.Body's antioxidant defense mechanisms to fight through it arise. Parameters reduction of glucose control and serum lipids in patients with the use of medicinal plants is important. The aim of this study was to evaluate the antioxidant effect of the aqueous extract of cinnamon on pancreatic tissue of streptozotocin-induced diabetic male rats. In this study, the diabetes induced in 21 adult male Wistar rats in the weight range 200 ± 30 with an intra-peritoneal injection of streptozotocin (mg/kg 50). Animals in three groups of 7 digits healthy, diabetic control and diabetic rats treated with the extract, respectively. Fasting mice were injected with a dose of 50 mg/kg of streptozotocin (STZ) intraperitoneally was diabetic. After 21 days of treatment with the extract at a dose of 200 mg / kg of malondialdehyde (MDA), FRAP and catalase were measured in three groups of pancreatic tissue. In order to perform statistical analyzes were performed using SPSS software. All data are presented as mean ± standard error (SE ± Mean) was expressed. ANOVA test and the Tukey's test were used for comparisons between groups. Significant in statistical work (0.05> P) was considered. The results showed that treatment with cinnamon extract increases the FRAP and MDA decreased in male rats, but in this study the changes in catalase activity was observed.

Tetracyclines are antibiotics that are widely used. Tetracycline, easily passes the blood-brain barrier and protects the nervous system due to its specific chemical structure. Vitamin E is one of the fat-soluble vitamins. This vitamin helps prevent the progression of Alzheimer's disease. The purpose of the present study is to investigate the effects of tetracycline and vitamin E on spatial memory, learning, and depression. In this experimental study, 21 male Wistar rats were randomly divided into 3 groups of 7 each. The effect of tetracycline and vitamin E on memory, learning, and depression was investigated using 8-arm radial maze and elevated plus maze. Data were analyzed by one-way ANOVA and Tukey's statistical tests. Statistical significance level was considered p<0.05. After 21 days of treatment, it was shown that tetracycline antibiotic has a significant effect on memory. Also Vitamin E significantly reduced depression and its protective effect decreased the adverse effect of tetracycline. In the treatment group, vitamin E along with tetracycline had a significant effect on memory loss. The results of the present study indicated that use of vitamin E can reduce depression by its antioxidant and protective effects. Tetracycline also significantly increases memory. Therefore, according to the results, it is suggested that tetracycline be used along with vitamin E to reduce negative effects of the drug.

Background and Food with high cholesterol and saturated fatty acids may play a role in formation of plaque in the walls of arteries. The purpose of this study was to evaluate the effects of dietary supplements containing different amounts of cholesterol and phytosterol on blood lipid profiles in rat. In this study, 28 adult male rats were divided into 4 groups (n=5 per group). They were kept under a 12 h light/dark cycle at constant temperature with food and water available freely throughout the study. The dietary supplement containing ghee, barley oil and olive oil were added to the foods in three groups. After 21 days of treatment, to measure various blood biochemical parameters and performing histological studies, the process of extracting blood from the heart and cardiac tissue were performed, respectively. Data were analyzed by one way ANOVA and Tukey test in SPSS software. The significance level was considered as P<0.05. Serum cholesterol and LDL-c in the groups treated with ghee, olive oil and barley oil decreased significantly compared with that of the control group. HDL-c levels increased slightly in the group receiving ghee supplement. Also, the level of TG decreased significantly in the group treated with barley and ghee. In histological study of heart tissue, signs of atherosclerosis were found more in the group treated with barley. The results showed that consumption of ghee and olive oil have positive impact on blood lipid profiles and some of the factors associated with atherosclerosis.

Multiple Sclerosis (MS) is an autoimmune disorder in central nervous system characterized by neuroinflammation. In this study, the effect of Epigallocatechin-3-Gallate (EGCG) on learning and spatial memory was investigated following a demyelination induction using lysolecithin injection into the rat hippocampus. In this experimental study, for the demyelination induction, 2µl lysolecithin (LPC) was injected streotaxically into the dentate gyros. Then, animals received 30mg/kg catechin for 7, 14, and 28 days after demyelination induced by lysolecithin. Subsequently, the spatial memory was investigated by a radial maze. Repeated measurement used for statistical evaluation. The impairment of the spatial memory was more significant 7 and 14 days after the LPC injection. Hence, food finding time has increased significantly compared to the control group (P<0/001). Moreover, the administration of EGCG has improved the spatial memory so that receiving EGCG for a longer time e.g. 28 days after demyelination induction can significantly recover the learning and spatial memory compared to the LPC-receiving group (P<.001). The long-term administration of EGCG can improve the spatial memory and learning of rats. Hence, it can be suggested as a treatment for demyelinated disease such as MS.

Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids to normal level is clinically very important. We decided to assess the preventive role of aqueous extract of Berberis Integerrima root on the serum levels of glucose، insulin، and lipid profile in Streptozotocin (STZ) -induced diabetic rats. Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root extract for 6 weeks); 3- diabetic (D) (they received STZ، 65 mg/kg BW /i. p.); 4- diabetic + barberry before (D+Bb) (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks); and 5- diabetic + barberry after (D+Ba) (three days after STZ injection، they received barberry root extract for 3 weeks). The experimental groups received barberry root extract (500 mg/kg bw) intra gastric by gavage for 6 weeks and the experimental period for each rat was 6 weeks. Diabetic rats showed a significant increase in serum glucose، total cholesterol، triglycerides، LDL-C، and food intake as well as a decrease in HDL-C، body weight and serum insulin، compared to the other groups. Administration of the barberry root extract in diabetic rats restored these changes towards normal to some extent. In this study، for the first time، we showed that the administration of the barberry root extract before diabetes induction resulted in better amelioration in the serum levels of glucose، insulin، and lipid profile، compared to the group receiving it after induction: this indicates that the barberry root extract can play both a preventive and a therapeutic role in such patients.

Non steroidal anti inflammatory drugs (NSAIDs) are widely used to reduce inflammation, pain and fever. The present study was organized to induce an experimental inflammation in an animal model, using a putative biological oxidant, Peroxynitrite (ONOO-) and to study the effects of Flunixin meglumine and Ketoprofen on the pressure-induced pain. For this purpose, 24 male guinea pigs were divided into 4 groups each consisting of 6 animals. Three groups (groups 2, 3 and4) were injected Peroxynitrite and one group (the first: control) received physiological salt solution subcutaneously in the paw. After induction of a local inflammatory response, Flunixin meglumine (1mg/kg) and Ketoprofen (2 mg/kg) were injected to the second and third groups, 5 times with 12h intervals. The first and the fourth groups were injected saline solution in the same manner. Pressure analgesiometry was performed before and 1 hour after injections. The animals in all 3 groups treated with Peroxynitrite demonstrated an increased sensitivity to painful pressure (P<0.05). Both NSAIDs decreased the pain sensation dramatically after the 1st and the 2nd injections but, not after the 3rd, 4th and the 5th injections. The study suggested that NSAIDs may be helpful in reducing pressure-induced pain in animal model in early hours of the treatment, whereas the effect subsides over time and ends up after a few days. This effect may be of importance in humans who receive these kinds of drugs for a long period as they may not be effective in reducing pain after a while.

Epilepsy in humans is characterized by seizures companied partly by mental and emotional deficits. The most commonly forms of affective psychopathology accompanying seizure disorders in humans are depression and anxiety.Neuroinflammation is implicated in etiology or neuropathology of not only neurodegenerative disease but also a number of psychiatric disorders. Kindling is a chronic model of epilepsy. The aim of present study was to explore the effect of aspirin an anti-inflammatory drug on anxiety-like behavior in kindled rat. Methods & materials: Three groups of male rats (8 in each group) were administered daily with vehicle, aspirin 15 and 30 mg/kg, orally. Kindling was induced by subconvulsive dose of pentylenetetrazol (PTZ 40 mg/kg,i.p).Kindling parameters in the control and aspirin treated groups were determined. One separate group of rats received vehicle only throughout the study and served as control group. The elevated plus maze was used in combination the percentage of time spent in the open arms of the maze (OAT%) and percentage of entries in to the open arms (OAE %) to measure anxiety. increase in OAT% and OAE% indicate an anxiolytic effect (reduction in anxiety). Kindling significantly (P<0.05) results in increased anxiety response in rats for at least 24h following the last seizure (Decrease in OAT% and OAE %).Daily aspirin (15,30 mg/kg) treatment induced a significant (P<0.05) increase in OAT% and OAE%,there by displaying decreased anxiety in kindled rats. Also significant change in the activity of animals was observed. Results of the present study show that aspirin could improve elevated levels of anxiety in kindled rats.

Several clinical studies indicate deterioration of sexual behavior in diabetic patients. The pathophysiological mechanisms of diabetic sexual impotence remain obscure. In this study the therapeutic effects of melatonin on sexual behavior were investigated through the central serotonergic system in diabetic rats. In this study, 30 male adult Wistar rats, weighing 200±20 g, were used. Animals were divided into three groups, the controls (C), diabetic (D) and the melatonin-treated diabetic (M) group. Experimental diabetes was induced by intraperitoneal injection of 50 mg/kg streptozotocin. Melatonin was injected (10 mg/kg i. p.) after 3 days of streptozotocin injection for 30 days. At the end of the administration period, the sexual behavior of each male rat to an ovariectomized female rat was assessed for 40 min. Serotonergic type 2 (5-HT2A) receptor activities were investigated through spontaneous WDS behavior, and experimental data were statistically analyzed. First mount, first intromission and first ejaculation latencies significantly (p<0. 05) increased in diabetic rats as compared to controls. Melatonin treatment significantly (p<0. 05) reduced these responses in the M group, compared to the D one. Also the number of mounts, intromissions and ejaculations significantly (p<0. 05) decreased in diabetic rats compared to controls. Administration of melatonin significantly (p<0. 05) increased these activities in the M group as compared to the D one. Calculation of copulatory efficiency and the sexual activity index of each rat indicate that reproductive activity in diabetic rats was significantly (p<0. 05) less than other two groups. The number of WDS responses was significantly (p<0. 05) different in all three groups. Sexual dysfunction in diabetic animals was accompanied by decreasing of 5-HT2A receptor activities, and melatonin prevented the diabetes-induced sexual impotence by modulating of central serotonergic system activity.

Diabetes mellitus is often accompanied by chronic neuropathic pain. Studies indicate that oxidative stress has an important role in the appearance of neurological and behavioral changes in diabetes. Necessitating researching therefore the antioxidants effects in alleviation of diabetic neuropathic pain. In this study, 32 male Wistar rats weighing 200±20 g were used, and were divided into four groups: Control(C), melatonin(M), diabetic(D) and melatonin-treated diabetic(MD). Experimental diabetes was induced by intraperitoneal injection of 50 mg/kg streptozotocin (STZ). Melatonin was injected (10 mg/kg/day, i.p.) for 2 weeks, after 21 days of diabetes induction. At the end of administration period, nociceptive biphasic behavior in rats was assessed using the 0.5% formalin test, and then observed for up to 60 min, according to spontaneous flinching and licking responses. In this study, lipid peroxidation levels, glutathione-peroxidase and catalase activities were measured in spinal L4-S3 dorsal root ganglia. Experimental data were then statistically analyzed. Formalin-evoked flinching increased in both acute and chronic phases of pain in diabetic rats as compared to non-diabetic ones, whereas administration of melatonin reduced flinching frequency in both phases in MD rats. Total time of licking in diabetic rats was significantly (p<0.05) more than the control rats in both acute and chronic phases of pain melatonins injection significantly reduced this time in both phases of pain in the MD as compared D group, whereas was no significant difference between M and C rats in the indices mentioned. Assessment of dorsal root ganglia homogenates indicated an increase in Lipid peroxidation levels and a decrease in GSH-Px and CAT activities in the D group as compared to the controls (C). While melatonin administration ameliorated these in diabetic rats. Results suggest that oxidative stress contributes to appearance of pain in diabetes and melatonin, as an antioxidant, is effective in reducing the acute and chronic pain in diabetic rats.

Background and Objectives Rheumatoid arthritis (RA) is a chronic inflammatory disease which is characterized by joint swelling and synovial inflammation. C reactive protein (CRP) and ceruloplasmin (CP) are identified as important biomarkers of RA and various inflammatory diseases. Curcumin a widely used yellow color spice is the most active component of Curcuma longa L (Turmeric). Curcumin contains potent anti-inflammatory and antioxidant properties. The goal of this study is evaluation of the anti-inflammatory effect of curcumin on arthritic palm of rats and levels of the CRP and CP in the blood samples of arthritis induced male albino Wistar rats. Methods Arthritis was induced by subcutaneous injection of Freund s Complete Adjuvant (FCA) into the palm of right rear foot of 8 different male albino Wistar rats. The rats were randomly divided into five groups after the injection. These groups were as follow: Group Ι control normal rats Group II carrier arthritic rats Group III arthritic rats which were given 30mg/ kg of curcumin orally seven days prior to FCA injection Group IV arthritic rats treated with 30mg/kg of curcumin Group V arthritic rats treated with 3 mg/kg of indomethacin. All the groups except group III received oral treatment with curcumin seven days after FCA injection and the treatment was continued fourteen days thereafter. The rear foot thicknesses of all the rats were measured on days 1 5 10 15 20 after FCA injection. The rats were destroyed after 20th day and their blood samples were collected. Results The results of this study indicate that curcumin significantly decreases swelling of the rats rear foot (p

The hippocampus has been implicated in many learning and memory functions including spatial memory. Leptin is a peptide hormone secreted by adipose tissue. Some studies have also suggested that leptin affect learning and memory. The present study is scheduled to investigate the effect of intraperitoneal (IP) injection of different doses of leptin on spatial memory formation. 60 male rats were divided into 6 groups in our experiments: (1) control, (2) sham, and (3), (4), (5), (6) intraperitoneal injection of 0.0, 0.1, 0.25 and 0.5 mg/kg doses of leptin respectively. All groups were trained in Morris water maze for two days. Learning parameters were compared between groups. Our results showed there were significant differences between sham group and test groups in spatial learning. Together our findings suggest that intraperitoneally injection of leptin improved spatial memory in rat. Leptin shows its highest effect with medium doses.

Peroxynitrite, produced naturally in the body from a reaction between nitric oxide and superoxide anion, possess destructive effects against microorganisms. In excess concentrations, however, it may also lead to cellular damage and inflammatory reactions in the host. Non - sterodial anti inflammation drugs (NSAIDs) are used widely in therapy for their anti-inflammatory, analgesic and antipyretic properties. Meanwhile, their adverse effects on endocrine functions should be taken into account. This project aims at the following goals: 1) establishing a new animal model of peroxynitrite-induced inflammation, 2) studying the effect of two selected NSAIDs on these parameters.3) investigating the possible effect of this oxidant on the blood levels of cortisol and glucose. 24 male guinea pigs were divided into 4 groups (6 animals in each group). Three groups were injected peroxynitrite and the last group, control group, given physiological salt solution in the paw subcutaneously. Following induction of a local inflammatory response, flunixin meglumine and ketoprofen (0.5 mg/0.5 ml) were injected to second and third groups, 5 times with 12h intervals. First and fourth groups were injected saline solution with the same manner. Animals were anesthetized with thiopental (60 mg/kg, i.p.) and a blood sample was collected by heart puncture. The glucose and cortisol levels of blood were determined by routine laboratory techniques. Blood glucose concentration in the animals that only injected peroxynitrite was less than the control group. In addition, groups which were given drugs had statistically higher levels of glucose in their blood more than the others. Although, cortisol levels were lower in the test groups compared to the control group, these differences were not significant statistically. The results of current study showed that both peroxinitrite and NSAIDs decrease the cortisol levels of blood. These findings can be a possible explanation for the lower levels of cortisol in the blood of patient who receive nitro glisirin as well as osteoarthritis patients that mainly take NSAIDs. In the study, the glucose levels of blood in animals given drugs were more than the control groups.