هانیه سادات شهاب زاده

This experiment was carried out to investigate the effect of methyl jasmonate and salicylic acid on the antioxidant production and physiological-biochemical properties of Chelidonium majus under drought stress condition. For this, an experiment was conducted as factorial based on completely randomized design with four replications in Imam Khomeini International University during 2016-2017. Three factors included drought stress in two levels (non-stress and drought stress with 100% and 50% field capacity irrigation, respectively), elicitor in three levels (control, 100 μm methyl-jasmonate and 2 mM salicylic acid) and plant organs (root, stem and leaf). Analysis of variance and mean comparisons showed that methyl-jasmonate and salicylic acid reduced the activity of CAT enzyme. Both salicylic acid and methyl-jasmonate did not affect the activity of SOD, but methyl-jasmonate increased the activity of GPX and salicylic acid reduced APX activity under drought stress. Also, methyl-jasmonate and salicylic acid reduced APX activity compared to control treatment. The highest amount of protein content was observed in root and then in leaf. Salicylic acid increased total protein content up to 34% compared to control treatment. Both Methyl-jasmonate and salicylic acid increased chlorophyll chlorophyll, carotenoids, flavonoids and anthocyanin compared to control treatment, but salicylic acid was superior in increasing of chlorophyll and carotenoids whereas methyl-jasmonate was superior in increasing of flavonoids and anthocyanin. In conclusion, methyl-jasmonate and salicylic acid have increased the ability of celandine in response to drought stress, and also, in addition to the protective effect against drought-induced oxidation, they increased photosynthetic pigments and some of metabolites.

In this study, the effects of salicylic acid and methyl jasmonate as two inducers were studied on antioxidant activity, photosynthetic pigments and flavonoids under salinity stress in Chelidonium majus L. This experiment was conducted as a factorial in a completely randomized design with four replications at Imam Khomeini International University during 2017. The elicitors were treated as foliar and mixed with irrigation. The factors studied included elicitors at three levels (distilled water as control, 2 mM salicylic acid, and 100 µM methyl jasmonate), salinity stress at two levels, urban water as control (EC=0.62 ds/m), salinity at 30 mM NaCl (EC=3.36 ds/m), and plant organs at three levels (leaf, root, stem). The analysis of variance showed that the main effects of salinity, elicitors, organs and their interactions were significant for most of the traits. The results of mean comparison for elicitors showed that methyl jasmonate increased carotenoid, flavonoids and anthocyanin. Both salicylic acid and methyl-jasmonate treatments reduced the amount and activity of catalase, superoxide dismutase and guaiacol peroxidase enzymes under salinity stress. In mean comparison of the triple interaction effects, salinity놩扳⥢ acid闦 was the best combination of factors for chlorophyll (1.68 mg.g-1 FW) and carotenoid (0.54 mg.g-1 FW) contents. Also, the maximum content for anthocyanin (5.7 OD.gr-1 FW) was observed in non-salinity駫懊 jasmonate闦 and for flavonoids in salinity麉hormone (3.42 OD.gr-1 FW), non-salinity놩扳⥢ acid (3.27 OD.gr-1 FW) and salinity駫懊 jasmonate (3.2 OD.gr-1 FW) in leaf, respectively. The amount of total protein increased in salinity놩扳⥢ acid (56.72 mg.g-1 FW) and salinity駫懊 jasmonate (53.27 mg.g-1 FW) in contrast to reduction in salinity麉hormone (43.35 mg.g-1 FW). In conclusion, by applying the two mentioned elicitors, greater celandine does not need to increase the antioxidant defense system and will potentially improve the yield of biomass and some secondary metabolites under salinity stress.