فهرست مطالب وحید حسن وند

Sesame curly top virus (SeCTV) is a new species in the genus Turncurtovirus (family Geminiviridae). To date, sesame was the only natural host of the virus. During a survey for identification of geminiviruses infecting crop, vegetable or ornamental plants in south-eastern Iran, the sesame curly top virus infection of alfalfa, bean, eggplant, faba bean, blanket flower, radish and watermelon was confirmed by PCR and sequencing. The full-length genome of seven new SeCTV isolates share >97 and ~87-88% nucleotide identity with other sequences from Iran and Pakistan, respectively. An infectious clone of SeCTV was constructed, biolistically-or Rhizobium-inoculated to several host plant species and the pathogenesis of the virus was demonstrated. Results of this study indicated that, similar to known turncurtoviruses, SeCTV has a wide natural host range in Iran and among the alternative hosts, the rate of infection and severity of disease in watermelon are remarkable.

Faba bean necrotic yellows virus (FBNYV) (Nanovirus, Nanoviridae) is a common nanovirus of leguminous plant in Asia, Africa and Europe continents. In this research, natural hosts of FBNYV in three eastern provinces of Iran have been studied and full-length genome components of a Rabor isolate (Kerman province) has been sequenced. To identify FBNYV natural hosts, leguminous plants and leaf vegetables were sampled in Kerman, Razavi Khorasan and Northern Khorasan, and the FBNYV infection of the samples was tested by PCR and/or nucleotide sequence of DNA-S component. Results indicated that lentil, faba bean, broad bean, chickpea, alfalfa, fenugreek, parsley and dill are infected with FBNYV and DNA-S of seven isolates from seven plant species share 89.6-99.7% nucleotide sequence identities. Among infected hosts, full-length genome components of a chickpea isolate of FBNYV from Rabor (Kerman province) was determined and compared with the related components of the GenBank isolates. Accordingly, all genome components of the Rabor isolate shared 88.57-99.81% nucleotide sequence identities with the corresponding sequence of other Iranian and GenBank isolates. To conclude, FBNYV has a broad host range in leguminous plants and even can infect non-leguminous leaf vegetables. It is the first report of the FBNYV infection of fenugreek, parsley and dill in the world.

The genus Begomovirus is the largest genus of the family Geminiviridae. In this research, a common marshmallow (Malva sp.) sample with the begomovirus-like symptoms including crinkled and curling leaves was collected in Jiroft. Total DNA was extracted using the CTAB method and subjected to next generation sequencing approach. Sequence analysis of NGS data indicated that the common marshmallow sample is infected with the Cotton leaf curl Gezira virus (CLCuGV) and Cotton leaf curl Gezira betasatellite. To confirm the sequence data, the full-length genome of CLCuGV and the associated betasatellite were amplified using rolling circle amplification method and the RCA kit followed by cloning and Sanger sequencing of ~3kb amplicon. Sequence data indicated that genomes of the Iranian CLCuGV isolate and the associated betasatellite are 2761 and 1355 nts in length. To identify other CLCuGV hosts, a sunflower sample showing severe crinkling and cup-shaped leaves and five okra samples with severe leaf curl symptom were collected from greenhouse and/or open farms in Jiroft and Dezful (Khuzestan province), respectively. DNA of samples was extracted and subjected to the PCR assay using primer pair of CLCuGV-F/CLCuGV-F followed by detection of 730bp in length amplicon. The associated betasatellite with the okra and sunflower samples was detected by RCA and sequencing of the resulted amplicon.