پروانه صفدریان

Sperm quality evalution is a valuable tool to study the reproduction potential of male animals and is important for breeding and preservation of medical species. In this study, 66 Zanjani male snakes were collected from two mountainous and cold habitats, Bostanabad in eastern Azarbaijan and Tarom of Zanjan Province of Iran, in year 2011 to 2012. Morphological characteristics of animals, including body weight, body length from snout to vent, specifications of vas deferen duct and sperm parameters including: concentration, motility, viability of sperm and also the presence and absence of ductal sperm were examined. Results show the morphology of vas deferens in Zanjani viper consists of three parts: proximal, median and distal. The longest values of vas deferen (right and left) lengths in Bostanabad viper was observed in the autumn and the shortest lengths in the spring, whereas these values for Tarom snakes were opposite. The highest mean percentage of sperm concentration in Bostanabad samples was observed in the spring and in Tarom samples was in the autumn. According to the results we can recommend concentration of mature sperms with suitable reproductive parameter are highest in the vas deferen in May, October and November and sperms with highest motility are present in the distal part of the duct in the spring and autumn, and it seems animal is ready for mating.

Inroduction & According to need to preserve the native breed snakes, in order to use humanitarian and environmental terms, In 2008, the environmental protection in vulnerable categories of the Red List Department of Environment (IUCN) (14),and serpents Zanjani is required for the survival of the species reproductive parameters noted. For this purpose to study the reproductive parameters of Zanjani viper(Iran), microscopic and macroscopic level: Check the male reproductive tract, sperm vas channel concentration, motility and morphology, weight, testicular volume, the evaluation of spermatogenic cells in the tubules and measuring weight and body length, tail length is studied. Viper Zanjani ring of 11 males in the winter of 2011 Bostanabad area in East Azerbaijan, which is mountainous and cold climate, samples were taken. The results showed that the mean maximum body weight of 248.33 ± 32.81 g, body length 76.41 ± 4.12 cm in February, tail length 4.87 ± 0.62 inches in February and maximum size of the right testis 666.13 ± 110.58 cubic millimeter of the Month for February and left 667.52 ± 189.08 cubic millimeter was seen in March. The highest average length of the left and right channels, respectively, 19.5 and 16.66 ± 2.54 cm was corresponding to the months of January and February. Maximum average sperm concentration in February 15×104/ml, morphology January %88.61±12.18 normal sperm motility and the highest 33.79 ± 22.91 of March the motility of the proximal to the distal face was increased. According to the findings viper Zanjani male reproductive tract morphology, including three in the proximal, median and distal to the That looks like a pit viper, Crotalus durissus terrificus species of the temperate zone of south-eastern Brazil is and the rate of sperm production in the months of February and March may be due to the mating stage (autumn) is.