مقالات رزومه زهرا حیدری
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Background
Intestinal parasitic infections are still a considerable global public health problem. We aimed to determine the frequency of intestinal parasitic infections among people referring to the central laboratory of Meshkin Shahr City, Ardabil Province, Iran.
MethodsIn this cross-sectional survey, 460 fecal samples were collected randomly from persons referred to the central laboratory of Meshkin Shahr City, from January to June 2022. The samples were examined by direct wetmount, Trichrome and modified Ziehl-Neelsen staining, formalin ethyl acetate sedimentation, and agar plate culture.
ResultsThe frequency of intestinal parasites was 15.7% (72 out of 460 cases), with some people with numerous intestinal parasites. The frequency of protozoan infections (13.9%) was higher than the helminthic infections (2.6%). Blastocystis spp. (8.1%) was the most prevalent detected intestinal protozoan. Entamoeba coli (5.7%), Dicrocoelium dendriticum (2.2%), Giardia lamblia (1.5%), Fasciola spp. (0.2%), and Hymenolepis nana (0.2%) were other detected parasites.
ConclusionIn- spite of betterment of the health condition in Iran and reduction of parasitic infection, intestinal parasitic infections are still a considerable public health issue in some parts of Iran.
Keywords: Intestinal parasitic infections, Prevalence, Protozoans, Helminths, Iran} -
زمینه و هدف
ماهی ها یکی از مهم ترین منابع انگل های مشترک بین انسان و دام در سراسر جهان هستند. این مطالعه با هدف تعیین انگل های کرمی ماهیان صید شده از رودخانه ارس انجام شد.
روش کاردر این بررسی طی سال های 1399-1400 تعداد 100 ماهی شامل 20 عدد ماهی کپور معمولی، 10 عدد کپور نقره ای، 5 عدد کپور سرگنده، 12 اسبله، 5 عدد ماش ماهی، 15 عدد کلمه، 3 عدد سوف، 22 عدد زردپر، 8 عدد سیم از رود ارس صید شد. تمام قسمتهای بدن ماهی (فلسها، آبششها، چشمها، ماهیچهها، شکم، دستگاه گوارش) از نظر انگلهای کرمی بررسی شدند. پس از شفاف سازی و رنگآمیزی موقت با لاکتوفنول-آزوکارمین، خصوصیات مورفولوژیکی کرمها با استفاده از میکروسکوپ کالیبره شده مجهز به دوربین مورد تجزیه و تحلیل قرار گرفت.
یافته هابعد از بررسیهای انجام شده و ارزیابی نمونهها، تعداد 18 (18%) عدد از ماهی ها آلوده به انگل بودند. انگل های کرمی یافت شده در ماهی ها شامل پلروسرکویید لیگولا اینتستینالیس در کپور معمولی 40%، کلمه 13/33%، سیم 12/5% و بوتریوسفالوس در کپور معمولی 5% و دیپلوزون در کپور معمولی 5% در کلمه 6/7% و داکتیلوژیروس در کپور معمولی 10%، در سیم 12/5% و متاسرکرکلینوستوموم در کپور معمولی 5% بود.
نتیجه گیریدر بین انواع ماهی های بررسی شده بیشترین میزان آلودگی به انگل های کرمی در کپور معمولی بود. همچنین در بین انگل های کرمی یافت شده بالاترین فراوانی مربوط به لیگولا اینتستینالیس و بعد از آن داکتیلوژیروس می باشد.
کلید واژگان: کرم های انگلی, ماهی, رود ارس}Background & objectivesFishes are one of the most important sources of zoonotic parasites throughout the world. This study aimed to determine helminthic parasites of Fish caught in the Aras River.
MethodsDuring 2020-2021, a number of 100 fishes including 20 Cyprinus carpio, 10 Hypophthalmichthys molitrix, 5 Hypophthalmichthys nobilis, 12 Silurus glanis, 5 Aspius aspius, 15 Ratilus rutilus, 3 Sander lucioperca, 22 Luciobarbus capito, and 8 Abramis brama were caught. All parts of the fish body (scales, gills, eyes, muscles, gastrointestinal tract) were examined for Helminthes parasites. After clearing and temporarily staining with Lactophenol-Azokarmin, morphological characters of worms were analyzed using a calibrated microscope equipped with a camera.
ResultsAfter examining and evaluating the samples, 18 (18%) of the fish were infected with at least with one helminthic parasite. The species and percent of infection were as follows: Ligula intestinalis plerocercoid: Cyprinus carpio 40%, Ratilus rutilus 13.33%, Abramis brama 12.5% in the abdominal cavity. Bothriocephalus sp.: Cyprinus carpio 5% in the intestine. Diplozoon sp.: Cyprinus carpio 5% and Ratilus rutilus each 6.7% in gills. Dactylogyrus sp.: Cyprinus carpio 10%, Abramis brama 12.5% in gills. Clinostomum sp. metacercariae: Cyprinus carpio 5% infected with metacercaria in gills and abdominal cavity.
ConclusionAmong the investigated types of fish, the highest level of infection with helminthic parasites was found in common carp. Also, among the helminthic parasites found, the highest frequency is related to Ligula intestinalis, followed by Dactylogyrus
Keywords: Parasitic Helminthes, Fish, Aras River} -
Background
Toxocariasis is a worldwide-distributed helminth parasitic infection. This study aimed to evaluate the seroprevalence of human toxocariasis in children living in Ardabil Province, North-West of Iran.
MethodsA seroepidemiological study to evaluate human toxocariasis among urban and rural populations of Ardabil County, North-West of Iran using ELISA test was carried out from 2019 to 2020. The study population was 472 children (185 females and 287 males) aged between 5 and 15 yr old. Immunoglobulin G antibodies against Toxocara spp. were analyzed by ELISA test.
ResultsOf the 472 collected serum samples, 66 (14.0%) were positive for antibodies against human toxocariasis. The highest prevalence of infection was observed in children with age groups of 5-6 years. Data demonstrate higher seroprevalence among males (15.3%) than females (11.8%). Rural areas prevalence was significantly higher (24.4%) than the urban area (8.65%). The rate of the diseases was 22.8% in children having history of contact with dog.
ConclusionToxocariasis is prevalent in the children of Ardabil region. The present study can increase the awareness of the population about the risk of zoonotic diseases as well as the anthelmintic treatment of dogs by veterinarians and, more importantly, the control of stray dogs.
Keywords: Seroepidemiology, Human toxocariasis, Iran} -
Background
Cystic Echinococcosis is considered a cosmopolitan cyclozoonotic parasitic infection. This study aimed at evaluating the seroprevalence of human hydatidosis using ELISA test and find the role of mutable factors such as age, sex, occupation, residency in the broadcast of the parasites in rural Ardabil Province, North-West of Iran.
MethodsThe study population was 950 asymptomatic individuals selected randomly from urban and rural populations of Ardabil province, North-West of Iran by randomized cluster sampling in 2019-2020. Immunoglobulin G antibodies against Echinococcus granulosus spp. were analyzed by ELISA test. Data were analyzed using SPSS software and Multivariable logistic regression model.
ResultsOverall, 42 (4.4%) of the participants had anti E. granulosus antibodies in this region. High titer antibodies were most prevalent in people age group of >70 yr old, rural areas, females and people having history of contact with dog that showed significant difference. There was no significant association between the presence of Echinococcus antibodies and sex, occupation, having history of eating unwashed vegetable.
ConclusionThis is the first description of the seroprevalence of E. granulosus infection in the population in Ardabil Province, North-West of Iran. Obtained rate of hydatidosis approves the importance of diagnosing human cystic echinococcosis in these regions and it is expected that the authorities be careful to screen the disease.
Keywords: Seroepidemiology, Human hydatidosis, Iran} -
Background
Cysteine proteases of the liver fluke, Fasciola hepatica, participate in catabolism of proteins, migration of the fluke through host tissues and combat host immune system.
ObjectivesIn this study, we evaluated proteolytic activity of F. hepatica recombinant cathepsin L1 (rCL1) against gelatin and collagen as common substrates.
Material and MethodsThe coding sequences of F. hepatica CL1 were cloned and expressed in E. coli, in our previous study. The rCL1 was purified by nickel affinity chromatography with a HisTrap Column. The protein concentrations of the purified fractions were determined by Bradford assay. Rat collagen type-1 was treated with distinct amounts of rCL1 at 37 °C, overnight, and the byproduct was analyzed by SDS-PAGE. Furthermore, we used bovine skin gelatin as zymography substrate to evaluate the gelatinolytic activity of the purified rCL1.
ResultsRecombinant CL1 was capable to digest intact type-1 collagen within 24 h and the gelatinlytic activity of rCL1 was visible at approximately 37 kDa region, with optimal activity at acidified conditions (pH 4).
ConclusionFindings provide a possible mechanism by which a major secretory molecule of F. hepatica could be involved in parasite survival as well as its pathogenesis.
Keywords: Cathepsin L1, collagen, Fasciola hepatica, Gelatin, Recombinant enzyme}
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