فهرست مطالب afshin taravati

Synovial fluid is composed of plasma ultrafiltration and hyaluronic acid secretion by synovial cells. Synovial fluid plays a role as softener and feeding cartilages without vessels. Infectious arthritis is one of the commonest arthritis and if the disease did not cure in the first days it would injure cartilages irreversibly. The goal of this study was identification of Staphylococcus aureus in synovial fluid of patients suspected to arthritis through PCR in Urmia city.
In this research synovial fluid contamination with Staphylococcus aureus and biochemical parameters such as the amount of glucose, protein and the number of white blood cells were studied. Amplification of nuc gene with the length of 279 bp using PCR method was applied to confirm Staphylococcus aureus isolation.
For this, 400 cerebrospinal fluid samples were tested from hospitalized patients with arthritis in two hospitals in Urmia city during 3 months, which out of them 109 of samples were contaminated with bacteria including: 78 of isolates were Staphylococcus aureus, 12 of them were coagulase negative Staphylococci, 4 of them were Streptococcus and 15 of them were gram negative bacilli. Also, results showed that the amounts of glucose in positive samples in comparison to the amount of glucose in synovial fluid were significantly decreased. The amount of protein and the number of white blood cells in synovial fluid of positive samples were significantly higher in comparison to normal synovial fluid.
Results showed that Staphylococcus aureus is the most common agent at infections arthritis, therefore it is recommended to use an experimental treatment for Staphylococcus aureus prior to final results.

Escherichia coli is the member of Enterobacteriaceae family and is one of the most important and common species of Escherichia in medicine. Escherichia coli has high potential in creating different intestinal and extraintestinal diseases in human and animals. The goal of this research determination of phylogenetic groups of Escherichia Coli isolated from human urine in Urmia city.
In the present research, 950 urine samples were studied from hospitalized patients in Urmia city. The urine sample were inoculated on MacCankey agar and blood agar and incubated at 37°C. Positive urine cultures was identified by standard laboratory methods involving morphological characteristics and biochemical tests. To determine the phylogenetic group, multiplex-PCR (m-PCR) was used. Primers used in this study could successfully amplify genes, including yjaA, chuA and TSPE4.C2 with 211, 279 and 152 bp, respectively.
Fifty samples were positive after culture and bacterial isolation. Fifteen of isolates belong to group A (30%), 6 belong to group B1 (12%), 20 belong to group B2 (40%) and 9 of them belong to group D (18%).
The high number of bacteria in group B2 as extra intestinal pathogenic agent, indicative of having a care about choosing a treatment for such infections.