amin jaidari

Brucellosis or Malta fever is one of the most prevalent zoonotic diseases considered as a health and economic problem in many countries in the Middle East, including Iran. The consumption of contaminated milk, milk products and contact with infected animals are the main transmission ways of pathogenic Brucella strains among human. The aim of the current study was to determine simultaneous detection of Brucella abortus and Brucella melitensis in raw and none-pasteurized bulk cow milk tanks of traditional domestic dairy sale centres in Khorramabad, Iran.
In this cross-sectional study during October and November 2015, a total of 120 samples from raw and unpasteurized bulk cow milk tanks were collected from traditional domestic dairy sale centres in Khorramabad. To confirm the presence of Brucella genus among the samples, single PCR was carried out using B4 and B5 primers and multiplex PCR was then carried out in order to detect the B. abortus and B. melitensis spp..
The present study revealed that 10% of the bulk milk tank samples in traditional domestic dairy sale centres in Khorramabad were contaminated with Brucella.
Results of PCR assay showed that raw and unpasteurized bulk cow milk tanks of traditional domestic dairy sale centres in Khorramabad are the potential cause of human brucellosis in this region.

Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. Raw milk or dairy products that are produced from unpasteurized milk may contain virulent Coxiella burnetii. The objective of this study was to determine the prevalence rate of C. burnetii in raw and unpasteurized cow bulk tank milk samples of traditional domestic dairy products vendors in Khorramabad, Iran.
In this cross - sectional study a total of 120 raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors were collected from October 2015 to November 2015 and tested for C. burnetii used a nested PCR assay.
In this survey, 9 out of 120 (7.5%) raw and unpasteurized cow bulk tank milk samples were found PCR positive for C. burnetii.
The Results of this study indicate that raw and unpasteurized cow bulk tank milk samples in traditional domestic dairy products vendors are an important source of C. burnetii infection in Khorramabad.