فهرست مطالب atefeh sabori

Barley (Hordeum vulgare L.) is one of the widely cultivated cereals and several pathogenic factors affect its grain yield quantity and quality. The fungal pathogen Blumeria graminis f. sp. hordei (Bgh) causes powdery mildew, which is a common leaf disease, and identifying genotypes resistant to this disease is one of the goals of breeding programs to combat this disease. The present study was conducted to investigate the resistance of 104 barley genotypes (including 99 advanced lines of barley along with five control cultivars) to powdery mildew disease under field (in two planting dates) and greenhouse conditions. The assessed traits included the severity of the disease, the infection type, and the area under the disease progression curve (AUDPC). The results of analysis of variance of the data showed that the effect of genotype on all the evaluated traits was significant (P<0.01). Considering the evaluation of genotypes under the field on two planting dates for the severity of the disease, and the results of cluster analysis of genotypes based on the different traits investigated in the greenhouse, totally 15 and 19 genotypes were identified as the most resistant and sensitive genotypes to this disease, respectively. These identified genotypes can be used for introducing to the disease-prone regions and also exploited in the formation of breeding populations and as important sources of candidate resistance genes to this disease.

Limes have been consumed as fresh fruits and processing products, due to high quality and acidity in fruit juice. In recent years, the incidence of Witches Broom disease resulted in destroy of thousands Limes trees in the south of Iran. The best method for controlling this disease is planting tolerant genotypes. Therefore, in this study, genetic diversity of 33 different lime genotypes which didn’t showed Witches Broom disease in the field and preliminary test, with 10 commercial citrus cultivars as a control was evaluated by 17 pair primers of SSR and 11 primers of ISSR. According to results with Simple Sequence Repeat (SSR) markers, the highest Polymorphic Information Content (PIC) were observed in TAA1 and TC26 with 0.72 and 0.68, respectively and by using ISSR markers were in ISSR1, 809 and 810 with 0.49 which was identified as a the most appropriate distinguishing markers. Results showed that there were differences between Limes and other types of citrus based on kind of studied markers. On the other hand, all of Lime genotypes had high affinity with Mexican Lime and Persian Lime and grouped in a cluster. Therefore, most of acid limes in south of Iran belonged to Limes group.