فهرست مطالب atefeh sadeghi shermeh

Dendritic cells are very important in basic studies and vaccine research, but isolation and culture of these cells face challenges due to their small number in tissues. Since there is no standard method, we addressed some of the factors affecting the efficiency of dendritic cell isolation and culture from BALB/c mouse bone marrow.

Bone marrow cells isolated from femur and tibia, were seeded in plates and treated with GM-CSF. On day 3, half of the media was transferred to a new plate with fresh media plus GM-CSF. On day 5, cells were induced with CpG oligonucleotide. Cell survival as well as maturation markers of CD11C, MHC-II and CD86 were investigated by flow cytometry on day 5 (immature cells) and day 7 (mature cells).

It was found that the presence of endotoxin in the culture of immature cells, significantly reduces the recovery of dendritic cells (p value <0.05). Less than one million cells seeded in non-treated 6-well plates and transfer of cells to a new plate on day 3 increased the efficiency of immature cell retrieval by preventing early maturation and premature death. Further treatment of immature cells with CpG on day 7 was used to induce the Th1 pathway. Significant difference (p value <0.05) with control group showed that CpG induces maturation after treatment with GM-CSF (without IL-4).

Optimization of dendritic cell culture in terms of different culture conditions highly impacts the efficiency of immature cell formation and maturation.

Although tuberculosis (TB) is often curable, it remains one of the top 10 major causes of death worldwide. Unfortunately, in recent decades, the emergence of Mycobacterium tuberculosis (Mtb) strains resistant to anti-TB drugs (DR-TB) is one of the most critical factors in the treatment of tuberculosis. Therefore, the present study addresses the epidemiologic factors, laboratory and clinical findings affecting the development of tuberculosis and, in particular, resistant forms of it. In this study, laboratory and clinical data were collected from 67 patients with drug-susceptible TB (DS-TB), 20 patients with DR-TB admitted to the Masih Daneshvari hospital and 30 healthy controls during one year (2015-16). The disease was confirmed by an infectious specialist according to clinical history, physical examination, radiological and laboratory findings. TB patients were divided into two groups: DS-TB and DR-TB, based on how they responded to treatment. Statistical analysis was performed using SPSS software. The incidence of DR-TB is associated with several factors, including history of previous treatment and contact with the TB patient. There were also significant differences between the three groups regarding the rate of erythrocyte sedimentation (ESR), C-reactive protein (CRP) and adenosine deaminase (ADA). According to the results, it is recommended that in high risk patients (with a history of previous treatment and contact with the TB patient, etc.), treatment should be done with greater accuracy and sensitivity. Also, attention to the differences in the clinical and laboratory findings of two DS and DR-TB groups may help in diagnosis and better treatment of the disease.

Lung cancer is the fifth most common cancer in Iran, with 10.5 and 1.5 per 100,000 in males and females, respectively. The study of latent tuberculosis infection (LTBi) in patients with lung cancer is significant due to the possibility of its activation during chemotherapy and increased mortality. Therefore, the aim of this study was to compare the prevalence of LTBi in patients with lung cancer with healthy individuals.
In this case-control study, which was performed from 2016 to 2017, 29 patients with newly diagnosed lung cancer and 40 healthy subjects were studied. Demographic data of all subjects were recorded in questionnaire. IGRAs (Quantiferon) were used to determine the LTBi.
The results of this study showed that prevalence of LTBi were 24.1% in lung cancer and 22.5% in the healthy group. The results of this study showed that smoking had a significant relationship with the risk of lung cancer and also LTBi infection in healthy people (p Since LTBi activates under chronic conditions such as malignancies, and because of high rates of LTBi in patients with lung cancer, it is recommended that screening for LTBi in high-risk individuals such as Lung cancer patients should be done before any treatment. Considering the role of smoking in lung cancer as well as the ability to develop LTBi in healthy people, attention to cessation of smoking in the health care program, prevention and treatment of pulmonary patients is recommended.