فهرست مطالب ayat h

Prevalence of heart valve defects about 25 to 30% in cardiovascular disorders. Mitral valve prolapse is one of the most common valve disorders that it′s prevalence about 4.2% in world population. Understanding the genetic basis of MVP can help to early diagnosis of it. Recently Smad6 and NFATc1 genes added to the list of genes which are involved in congenital heart disease and are very important genes interfering in valve cellular development. The aim of this research is to Study the upstream of Smad6 and NFATc1 genes polymorphism and correlation of it with Mitral Valve Prolapse in Iranian patients. After careful counseling and informed consent, peripheral blood samples were taken from 30 patients and 10 controls, and total DNA was extracted from white blood cells. Promoter regions of Smad6 and NFATc1 were amplified by standard PCR. PCR products were screened by Single Strand Conformation Polymorphism (SSCP) analysis. In this study we didn’t found any polymorphism in upstream region of Smad6 and NFATc1 genes in 25-50 old years Iranian patients with Mitral Valve Prolapse. Association between genetic polymorphism in promoter regions of Smad6 and NFATc1 genes and MVP is unknown and need to more study. Functional polymorphisms in this region may interfere with life and we propose study on neonates. A correlation between polymorphism in control region of these genes with childhood types of Mitral valve prolapsed is expected.

Each year, 111 million case of rotavirus diarrhea are reported. There is no specific therapy to treat rotavirus diarrhea, effective contraception should be considered. Considering water and food hygiene appears to have little effect on disease control, vaccination is the only way to reduce death caused by rotavirus diarrhea. In the current study, using modern genetic engineering method, a Polypeptid composed of several antigenic epitopes is made which can be used as a vaccine for rotavirus if its efficiency is cinfirmed. In this study, the prevalence of different species of viruses in Iran was examined. Serotype A was been the most common serotypes. The complete genome sequence was been, antigenic structure of the virus surface proteins were predicted by the of bioinformatics tools including Hex software and server Phyre. Four epitopes of the two virus surface proteins were identified and their genomes were sequenced. Primers were designed by use of software Genrunner in the way that each primer pair formed an epitope. Primers had overlapping areas, SOE-PCR method was used to connect them. In the gel electrophoresis, primers binding and the components growth are clearly seen. Combined fragment was cloned and initial expression was studied in prokaryotic system. The final product was place exactly at the desired place in comparison whit the marker according to molecular weight. Implementing SOE-PCR method in the study, encoding sequence of four epitopes of two rotavirus protein had bind together and new recombined piece was expressed in prokaryotic system. New recombined protein was in the expected place in terms of molecular weight.