فهرست مطالب elnaz zangani

A variety of organisms are present in Freshwater ecosystems that the knowledge of species distribution is essential to understand the ecological and evolutionary factors that determine patterns of biodiversity. The construction of a species distribution model is an alternative approach for the study of its geographic range. Therefore, in this study, the distribution of freshwater amphipods of the genus Gammarus in Iran was investigated using SDMs distribution models. The ensemble modeling approach (eSDM), GAM, GLM, MARS and RF models were used. AUC and Kappa criteria were used to evaluate the mentioned models. Also, climatic variables, topography, vegetation and soil quality were used for modeling purposes. The results showed that single models predicted the suitable habitat for taxon (AUC=0.90). The increasing level in soil organic carbon (SOC) and Bio18 variables lead to an increase in habitat suitability. Also, they are mostly recorded at an altitude between 1000 to 2500 meters, but, the increase or decrease in height does not have a linear effect on the habitat desirability in this genus. Therefore, the predicted distribution for this genus indicates the combined role of soil biomass, vegetation and precipitation parameters that can determine the distribution range of the genus Gammarus in Iran.

In recent years DNA extraction from various organisms is of strategic importance in downstream research such as molecular analysis phylogeny, and more recently new generation sequencing. This process will be even more important in organisms for which there is very limited genomic information.In the present work, genomic and mitochondrial DNA was extracted from almost 0.002 gram tissue of terrestrial isopods of Protracheoniscus species collected from different parts of Iran, based on phenol-chloroform procedure. The quality of extracted DNA was exactly evaluated by electrophoresis and nanodrop methods. Polymerase chain reaction for mitochondrial gene COI and its subsequent successful sequencing were regarded as another confirmation of DNA extraction from the given amount of these creatures tissue.The results of the present study can be used in phylogenetic studies or other genomic analyzes in rare species with very low tissue content due to the abundance of organisms with limited low genomic information.