فهرست مطالب ensie sadat mirsharif

Numerous studies have shown the anti-inflammatory, antimicrobial, anti-allergic, anti-cancer, anti-spasm, analgesic and wound healing effects of chamomile (Matricaria chamomile). In this study, the effect of aqueous and ethanolic extracts of chamomile on the vital activity of cultured leukocytes of patients with covid-19 was investigated.
After preparation of aqueous and ethanolic extracts of chamomile, peripheral blood mononuclear cells (PBMC) of patients with covid-19 with doses of 200, 300, 400 and 500 micrograms/ml of ethanolic extract of chamomile and doses of 400, 500, 600 and 700 µg/ml chamomile aqueous extract were treated. After 24 hours, the vital activity of PBMCs was measured by MTT method. To analyze the findings, one-way analysis of variance was used in SPSS software version 24. P<0.05 was considered significant.
In the current research, chamomile ethanol extract in doses of 500 micrograms/ml and aqueous extract in doses of 600 and 700 micrograms/ml led to a significant decrease in the vital activity of peripheral blood mononuclear cells compared to the control group.
Aqueous and ethanol extracts of chamomile significantly decrease the vital activity of peripheral blood mononuclear cells; This decrease in vital activity is dose-dependent, and with increasing dose, a decrease in vital activity is observed.

Researchers have already reported a high level of interleukin (IL)-6 in patients affected by Coronavirus Disease 2019 (COVID-19). In this study, we investigated the surge of IL-6 level and its association with the clinical and paraclinical markers in these patients.

The study sample comprised 205 confirmed hospitalized patients with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and 70 healthy volunteer individuals. Routine laboratory examinations, including hematology, biochemistry, and hormone analysis, as well as IL-6 level measurement, were conducted. The patients grouped into 5 based on their IL-6 levels. Then, they were compared with regard to their need for mechanical ventilator and clinical laboratory routine tests.

IL-6 levels were significantly higher in hospitalized patients compared with healthy individuals (P<0.001). The IL-6 level was approximately 10-fold of the normal range in 22.9% of the patients. Also, more than 56.1% of them signify IL-6 over 3-fold of the normal range. IL-6

Despite several reports on the clinical manifestations of sulfur mustard (SM) intoxication, there is no study on serum concentrations of thyroid hormones long-term after SM exposure. In this study, the changes in thyroid functioning parameters 20 yr after SM exposure were evaluated. This study is a part of a larger historical cohort study conducted in 2007 following 20 years of the exposure to SM, called Sardasht–Iran cohort study (SICS). We (SICS) comprised an SM–exposed group from Sardasht City, West Azerbaijan Province, Iran (n=169 as hospitalized group and n=203 as non-hospitalized exposed group); and control participants were selected from Rabat, a town near Sardasht (n=126). Peripheral blood samples were taken in fasting state and then the sera were separated. T4, T3, TSH, antithyroglobulin (anti–Tg), and antithyroid peroxidase (anti–TPO) concentrations in the sera were measured by the ELISA method. The mean of T3 concentration was significantly higher in the exposed than control group (0.88 ± 0.26 nmol/L vs 0.8 ± 0.25 nmol/L, P<0.001). The levels of TSH, T4, and T3up were not significantly different between the exposed and control groups. Thyroglobulin level was significantly higher in the exposed non-hospitalized group (56.07 ± 140.22 µg/L vs 17.66 ± 41.49 µg/L, P=0.004), but the level of anti–Tg and anti–TPO showed no significant differences between the two groups. More studies are needed on the alterations in thyroid hormones, their gene expressions, and mechanisms involved in SM exposure to clarify the causes of these alterations.

Preeclampsia is one of the most common complications of pregnancy that occurs after the 20th weeks of pregnancy. The pathophysiology of this disease is not exactly known. Transforming Growth Factor-Beta (TGF-β) and Nitric Oxide (NO) are the key regulatory factors secreted by Mesenchymal Stem Cells (MSCs). The aim of the present study was to evaluate the TGF-β and NO secretion by adipose-derived MSCs in normal and preeclamptic pregnant women.

The adipose tissues were collected from 10 preeclamptic patients and 10 age-matched normotensive controls at the time of cesarean section delivery. After isolation and expansion of MSCs, their capability of differentiation and immunophenotyping characteristics were assessed. Next, the release of TGF-ß was evaluated making use of ELISA sandwich method and Griess method was used to measure the level of NO.

Adipose derived MSCs in both groups were differentiated into osteocytes and adipocytes. The expression of CD90, CD73, CD44, and CD105 markers and lack of expression of CD-14, CD34, CD45, and HLA-DR markers in cells isolated from adipose in both groups was observed using flow cytometric analysis. The levels of TGF-ß secretion in preeclamptic women were significantly higher than those in control group, but the mean level of NO secreted by adipose derived MSCs did not significantly change in the two groups.

It can be concluded that significant increase in the secretion of TGF-β owing to MSCs in preeclamptic participants shows the importance of these cells in controlling immunological balance in these patients. Therefore, MSCs-based therapy seems to regulate TH1/Th2 balance in preeclampsia.

Preeclampsia is one of the most common complications of pregnancy that occurs after the 20th weeks of pregnancy. The pathophysiology of this disease is not exactly known. Transforming Growth Factor-Beta (TGF-β) and Nitric Oxide (NO) are the key regulatory factors secreted by Mesenchymal Stem Cells (MSCs). The aim of the present study was to evaluate the TGF-β and NO secretion by adipose-derived MSCs in normal and preeclamptic pregnant women. The adipose tissues were collected from 10 preeclamptic patients and 10 age-matched normotensive controls at the time of cesarean section delivery. After isolation and expansion of MSCs, their capability of differentiation and immunophenotyping characteristics were assessed. Next, the release of TGF-ß was evaluated making use of ELISA sandwich method and Griess method was used to measure the level of NO. Adipose derived MSCs in both groups were differentiated into osteocytes and adipocytes. The expression of CD90, CD73, CD44, and CD105 markers and lack of expression of CD-14, CD34, CD45, and HLA-DR markers in cells isolated from adipose in both groups was observed using flow cytometric analysis. The levels of TGF-ß secretion in preeclamptic women were significantly higher than those in control group, but the mean level of NO secreted by adipose derived MSCs did not significantly change in the two groups. It can be concluded that significant increase in the secretion of TGF-β owing to MSCs in preeclamptic participants shows the importance of these cells in controlling immunological balance in these patients. Therefore, MSCs-based therapy seems to regulate TH1/Th2 balance in preeclampsia.

Preeclampsia is one of the most common complications during pregnancy that occurs after 20th weeks of pregnancy in women with normal blood pressure. The pathophysiology of this disease is unknown. Due to changes in serum level of nitric oxide in women with preeclampsia and also the important role of mesenchymal stem cells in the secretion of nitric oxide as an immunoregulator، in this study، we aimed to evaluate the level of nitric oxide in secretions of adipose tissue-derived mesenchymal stem cells in women with normal pregnancy and patients with preeclampsia. Subcutaneous adipose tissue of 10 preeclamptic patients and 10 healthy pregnant women was collected during cesarean operation. After isolation and proliferation of mesenchymal stem cells، capablity of their differentiation and their immunophenotyping charactetistics were assessed. Then، their release of nitric oxide was evaluated using Griess method. Stem cells isolated from adipose tissue in both groups differentiated into osteocyte and adipocytes. Flow-cytometric analysis showed the expression of the markers CD90، CD73 CD44 and CD105 and lack of expression of the markers CD-14، CD34، CD45 and HLA-DR in both groups. No significant change was observed for the level of nitric oxide secretion in both groups. The present results suggest that nitric oxide secreted by mesenchymal stem cells do not have a significant contribution in variation of serum level of this factor and possibly do not play a role in the pathology of preeclampsia. However، such study with a higher sample size is suggested.