فهرست مطالب farshid rezaie

Microbial protein is used in animal and poultry feed. This protein is produced using microorganisms and different carbon sources such as methanol and methane. In this study, Methylophilus methylotrophus bacteria were cultivated in erlen and fermentor using methanol as a carbon source. The specific growth rate of bacteria was 0.0312 h-1 in erlen and 0.0047 h-1 in fermenter. After cultivation, the biomass was separated at optical density of 3.8 using a centrifuge at 7000 rpm, and qualitative and quantitative tests of microbial protein were performed on pellet. Amino acid profile, fatty acid profile, nitrogen content and crude protein were determined using high performance liquid chromatography, gas chromatography and Kjeldahl method, respectively. The results show that the amount of crude protein was 73.5%. The amount of ash was 29.8% and the total of essential to non-essential amino acids (including lysine, histidine, arginine, valine, leucine, isoleucine, phenylalanine, methionine and threonine) was 37.3% for the produced product. The amount of two non-essential amino acids, cysteine and tyrosine, was higher than the FAO standard and was 2.38% and 3.21%, respectively. The ratio of palmitic acid to the total fat of the product is 24.6% (w/w) and the ratio of linoleic acid and linolenic acid as unsaturated fatty acids is 9.2 and 2.3% (w/w) to the total fat of the product, respectively. The results obtained from this research showed that the product with a protein content of 73.5% seems an appropriate candidate for animal feed.

In Jan 2020, the outbreak of the 2019 novel coronavirus (SARS-CoV-2) in Wuhan, Hubei Province of China spread increasingly to other countries worldwide which WHO declared it as a public health emergency of international concern. Iran was included in the affected countries. Throat swab specimens were collected and tested by using real-time reverse transcription PCR (RT-PCR) kit targeting the E region for screening and RNA dependent RNA polymerase for confirmation. Conventional RT-PCR was conducted for the N region and the PCR products were sequenced by Sanger sequencing. The first seven cases of SARS-CoV-2 infections were identified in Qom, Iran. This report describes the clinical and epidemiological features of the first cases of SARS-CoV-2 confirmed in Iran. Future research should focus on finding the routes of transmission for this virus, including the possibility of transmission from foreign tourists to identify the possible origin of SARS-CoV-2 outbreak in Iran.