fatemeh aghaei
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Background
The emergence of COVID-19 in late 2019 swiftly led to a global pandemic, prompting urgent investigation into its multifaceted impacts. While prior studies have highlighted compromised sleep quality in hospitalized COVID-19 patients, this research delves into the enduring effects on sleep patterns for those with severe pulmonary involvement post-intensive care. This study addresses a critical gap in understanding the long-term consequences of the virus, emphasizing the importance of post-recovery care.
MethodsConducted as a cross-sectional study, we consecutively enrolled COVID-19 patients with over 20% pulmonary involvement admitted to the ICU at Shariati Hospital, Tehran, between June 2021 and January 2022. Demographic and clinical data were extracted from hospital records. After ethics committee approval, participants were contacted six months post-discharge to assess sleep quality using the Pittsburgh Sleep Quality Index (PSQI). Exclusions comprised obstructive sleep apnea patients and those with incomplete records.
ResultsOf the 60 eligible post-COVID-19 patients, 56 participated. Three individuals were unresponsive to calls, while one declined. Participants, primarily female (64.3%), had a mean age of 51.7 years. Notably, 80.4% exhibited sleep disorders six months post-discharge. Gender differences were observed, with females reporting higher scores in specific sleep quality components. Intriguingly, single individuals demonstrated a higher preference for sleep medication. These findings underscore the imperative for targeted interventions to improve post-ICU sleep quality in severe COVID-19 cases.
ConclusionThe high prevalence of sleep disorders six months post-discharge underscores the enduring impact of COVID-19 on sleep quality. These findings call for targeted interventions and comprehensive post-ICU care protocols that prioritize sleep health.
Keywords: COVID-19, Sleep Disorder, Sleep Disturbance, Pittsburgh Sleep Quality Index (PSQI), Pandemic, Sleep Quality -
Journal of Dentistry, Shiraz University of Medical Sciences, Volume:25 Issue: 4, Dec 2024, PP 334 -341
Statement of the Problem:
Dentin adhesion is challenging and needs modifications. Newly introduced nature-derived materials may be a useful solution in improving dentin adhesion. The use of natural antimicrobial agents for pretreating prepared dentin surfaces before restoration has become of interest.
PurposeThe present study investigated the effect of natural compounds on the shear bond strength (SBS) of resin composite adhesively bonded to dentin.
Materials and MethodSixty extracted human molar teeth were randomly divided into six groups (n= 10); Group I: two-step etch and rinse adhesive system, Adper Single Bond 2 (SB2) (experimental control), Group II: 2.5% Chitosan+etch+SB2, Group III: 15% Neem (Azadirachta indica)+etch+SB2, Group IV: 15% Tulsi (Ocimum sanctum)+etch+SB2, Group V: 2% Aloe vera+etch+SB2, and group VI: 2% Chlorhexidine (CHX)+etch+SB2. Resin composite (Z350, 3M ESPE USA) was placed over the prepared dentin surfaces and was light cured. Data analysis was performed using One-way ANOVA and post hoc Tukey's test (p< 0.05).
ResultsNeem specimens showed the highest mean SBS, statistically greater than the contr-ol (p= 0.042). Other experimental groups showed no significant differences in SBS comparison to the control. The mean SBS values of 2.5% Chitosan, 15% Neem, 15% Tulsi, and 2% Aloe vera were significantly higher than 2% CHX (p= 0.046, p= 0.001, p= 0.010 and p =0.002 respectively).
ConclusionDentin pretreatment with Neem may improve the bond strength of a two-step etch and rinse adhesive system. The application of Chitosan, Tulsi, Aloe vera, and CHX did not demonstrate significantly different SBS values from that of the Control, although CHX was lower.
Keywords: Resin Composite, Chitosan, Neem, Tulsi, Aloe Vera, Chlorhexidine, Shear Bond Strength -
Background
In this study, our aim is to investigate the complications caused by COVID-19 disease in the period of 1 month and 3 months after infection and recovery in patients aged 20 to 80 admitted to the special care department of Shariati Hospital in Tehran.
MethodsThe information of the patients in this research was collected between 1 and 3 months after discharge non-randomly and from among the samples that are available (consecutive), through phone calls and a prospective questionnaire including questions about complications such as fatigue, body pain, joint pain, anorexia, disorders, sleep, headache, depression, dizziness, palpitations, chest pain, cough, diabetes, skin disease, obesity, imbalance, neuropathy, neurological and mental problems, and other cases are collected.
ResultsIn this study, what was observed was those 4 complications of nausea and vomiting, diabetes, depression, and hypothyroidism were significantly related to hospitalization of COVID-19 patients in the hospital's special care department. The frequency of other complications was also investigated in this study.
ConclusionsAccording to the conducted study as well as previous studies, what is clear is that the frequency of complications caused by COVID-19 in patients admitted to the intensive care unit after discharge is high, and more studies should be done in order to reduce these complications.
Keywords: Pulmonary Complications, Post-COVID, Intensive Care Unit -
به منظور بررسی اثرات برخی تعدیل کننده های تنش (باکتری های محرک رشد و نانواکسید آهن و سیلیکون) بر عملکرد و برخی صفات فیزیولوژیکی و بیوشیمیایی تریتیکاله در شرایط محدودیت آبی، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوک کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 1400 اجرا شد. فاکتورهای مورد بررسی شامل آبیاری در سه سطح (آبیاری کامل به عنوان شاهد، قطع آبیاری در 50 درصد مراحل چکمه ای شدن (آبستنی) و سنبله دهی به ترتیب به عنوان محدودیت شدید و ملایم آبی به ترتیب کد 43 و 55 مقیاس BBCH)، کاربرد کودهای زیستی در چهار سطح (عدم کاربرد به عنوان شاهد، کاربرد آزوسپریلیوم، سودوموناس، کاربرد توام آزوسپریلیوم و سودوموناس) و محلول پاشی نانوذرات در چهار سطح (محلول پاشی با آب به عنوان شاهد، محلول پاشی یک گرم در لیتر نانو اکسید آهن، محلول پاشی50 میلی گرم در لیتر نانوسیلیکون، محلول پاشی توام نانواکسید آهن و نانوسیلیکون) می شدند. نتایج نشان داد کاربرد باکتری های محرک رشد و نانو اکسید آهن وسیلیکون در شرایط آبیاری کامل محتوای کلروفیل کل (48.16%)، عملکرد کوانتومی (36.05%)، محتوای نسبی آب (35.83%) و عملکرد دانه (43.28%) را نسبت به عدم کاربرد باکتری های محرک رشد و نانوذرات تحت شرایط قطع آبیاری در مرحله آبستنی افزایش داد. همچنین کاربرد باکتری های محرک رشد و نانوذرات تحت شرایط قطع آبیاری در مرحله آبستنی فعالیت آنزیم های کاتالاز، پراکسیداز و پلی فنل اکسیداز را (به ترتیب، 47.06، 55.69 و 36.53%) و محتوای پرولین، قندهای محلول (به ترتیب 45.41 و 46.93%) نسبت به عدم کاربرد باکتری های محرک و نانوذرات تحت شرایط آبیاری کامل افزایش داد. بر اساس نتایج این بررسی به نظرمی رسد باکتری های محرک رشد و نانوذرات می تواند بواسطه بهبود صفات فیزیولوژیکی و بیوشیمیایی، عملکرد دانه را در شرایط محدودیت شدید آبی افزایش دهد.
کلید واژگان: آنزیم های آنتی کسیدانی, اسمولیت های سازگار, سودوموناس, محتوای نسبی آب, نانواکسید آهنIntroductionWater limitation is one of the most important abiotic factors that can limit plant growth and yield due to production of reactive oxygen species (ROS) like H2O2 and the reduction of chlorophyll content. To protect against oxidative stress, plant cells produce both antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), and non-enzymatic antioxidants such as low weight molecules like proline, sugars and ascorbate. Also water limitation disturbs the mineral-nutrient relations in plants through their effects on nutrient availability and numerous of physiological and biochemical destruction in the vegetative and reproductive periods of plant development. Several strategies have been suggested in order to improve yield under abiotic and biotic stresses in plants, among them application of Plant Growth Promoting Rhizobacteria (PGPR) and nano particles such as nano iron-silicon oxide play a key role in yield improvement. A better understanding of physiological responses under water limitation may help in programs which the objective is to improve the drouht resistance of crop. During the course of these stresses, active solute accumulation of compatible solutes such as proline and the activities CAT, POD and PPO enzymes are claimed to be an effective stress tolerance mechanism. Therefore, the aim of this study was to evaluate the effects of bio-fertilizers and nano iron oxide and nano oxide on some physiological and biochemical (i.e., antioxidant enzyme activity, chlorophyll, protein, soluble sugars and proline) responses of triticale under water limitation conditions.
Materials and methodsAn experiment was conducted as factorial based on randomized complete block design with three replicates at the research farm of faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2021. The experimental factors were included of irrigation in three levels (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe and moderate water limitation respectively (BBCH 43 and 55 respectively), application of bio fertilizers in four levels (no application as control, application of Azospirilum, Pseudomonas, both application Azospirilum and Pseudomonas) and nanoparticles foliar application at four levels (foliar application with water as control, nano iron oxide foliar application (1 g.L-1), nano silicon oxide (50 mg.L-1), both application nano iron-silicon oxide). Psedomunas and Azospirilum were isolated from the rhizospheres of wheat by Research Institute of Soil and Water, Tehran, Iran. For inoculation seeds were coated with gum Arabic as an adhesive and rolled into the suspension of bacteria until uniformly coated. The strains and cell densities of microorganisms used as PGPR in this experiment were 1×108 colony forming units (CFU). In each plot there were 5 rows with 2 m long. In each experimental plot, two marginal rows and 0.5 m from beginning and ending of planting lines were removed data were measured from the middle lines. The used nano silicon-iron oxide had the average particle size less than 30 nm and special surface of particles was more than 30 m2.g-1. They were product of Nanomaterial US Research which was provided by Pishgaman Nanomaterials Company of Iran. Nano iron oxide and nano silicon powder added to deionized water and was placed on ultra sonic equipment (100 W and 40 kHz) on a shaker for better solution. Foliar application of nano silicon oxide and putrecine were done in two stages of period growth BBCH 21 and 30.
Results and discussionThe results showed that total chlorophyll content (48.16%), quantum yield (36.05%), relative water content (35.83%) and grain yield (43.28%) increased in dual application of bio fertilizers and nano particles foliar application under full irrigation conditions compared to no application of bio fertilizers and nano particles under irrigation cut off at booting stage. But under such conditions, electrical conductivity, hydrogen peroxide and malondialdehyde content decreased 35.67, 53.16 and 56.32% respectively compared to no application of PGPR and nanoparticles under irrigation withholding in booting stage. Also, the application of PGPR and nanoparticles under irrigation cut off in booting stage increased the activity of catalase, peroxidase and polyphenol oxidase enzymes (47.06, 55.69 and 36.53% respectively), proline and soluble sugars content (45.41 and 46.93% respectively) compared to no application of PGPR and nanoparticles under full irrigation conditions.
ConclusionBased on the results of this study, the application of plant growth promoting rhizobacteria and nonoparticle can increase grain yield of triticale under water limitation conditions due to improving biochemical and physiological traits.
Keywords: Antioxidant Enzymes, Compatible Osmolytes, Nano Iron Oxide, Pseudomonas, Relative Water Content -
Background
The aim of this study is to investigate the relationship between LDH and CRP levels and the mortality of COVID-19 (SARS-CoV-2) patients admitted to the SURGERY ICU at Shariati Hospital from 19 February 2020 to 19 February 2021.
MethodsOur study is a cross-sectional descriptive study. A total of 81 patients were enrolled in this study. We examined lab reports, including CBC parameters (WBC, neutrophil count, neutrophil percentage), other inflammatory markers ESR and CRP, and also studied the medical records of cases to collect details about age, gender, length of stay in SURGERY ICU, BMI, fever, survival status, comorbidity, intubation, and NIV. These patients were referred to Shariati Hospital, Tehran, from 19 February 2020 to 19 February 2021, regarding the relationship between LDH and CRP levels and the mortality of COVID-19 (SARS-CoV-2) patients admitted to the SURGERY ICU. The data obtained was analyzed using SPSS software, and a significance value of < 0.05 was considered.
ResultsIn this study, the data of 81 patients were considered. The data were obtained from the medical records of Shariati Hospital, Tehran. We used 11 variables to compare data from different patients that were recorded in the registry and system of medical records in Shariati Hospital, Tehran. The study population included 81 patients, of which 41 were female and 40 were male, ranging in age from 25 to 89. In total, 80.2% had mild disease versus 18.5% who had severe disease. Sixty-five patients survived, and 16 were admitted to the SURGERY ICU with endotracheal intubation and later died. The length of stay in the SURGERY ICU ranged from 1 to 25 days. Out of 81 patients, 52 (62.4%) had comorbidity, and 29 (35.8%) did not have comorbidity. Twelve patients (14.8%) received NIV, and 69 patients (85.2%) did not receive NIV. Most of the patients did not have a fever. The minimum and maximum levels of CRP were 4 and 416, respectively, and for LDH were 9 and 2401, respectively. The prognostic factors for the severity of COVID-19 infection identified in this study (CRP and LDH) help predict the course of the disease at an early stage. Elevated concentrations of CRP and LDH at admission were found to be associated with a higher risk for COVID-19 severity as they were significant (p-value =0.049 and 0.048, respectively).
ConclusionsThis study’s laboratory investigation showed that the SURGERY ICU patients had significantly higher values of inflammatory markers CRP and LDH than the non-SURGERY ICU patients. LDH and CRP were superior and effective biomarkers in predicting the severity of COVID-19.
Keywords: SARS-Cov-2, Lab Results, LDH, CRP, Lung Lesions -
Background
Central venous catheters (CVCs) are primarily used in oncology to protect peripheral veins and provide effective access to chemotherapy. The authors investigated the results of in-plane ultrasound-guided imaging compared to out-of-plane imaging in patients with oncology-related CVC placement in the oncology department of Shariati Hospital in Tehran.
MethodsThis cross-sectional analytical study was conducted on 50 eligible oncology patients over 6 months in 1400 at Shariati Hospital in Tehran. The patients were randomly divided into two groups: in-plane and out-of-plane, and the internal jugular venous catheter was inserted by using one of the methods above by an anesthesiologist. The necessary variables for statistical analysis were collected using predetermined questionnaires.
ResultsThe mean age of the patients was 73.42 years, with 25 female participants. The catheter insertion was successfully performed in all patients. The mean duration of catheter insertion in the study population was 2.289 seconds, the mean number of skin punctures was 1.7, and catheter placement was successful in 62% of patients on the first attempt. A total of 23 complications occurred, with carotid artery puncture being the most common. The catheterization method, whether in-plane or out-of-plane, did not affect the initial success of catheter insertion.
ConclusionsAccording to the findings of this study, internal jugular vein catheterization was successfully performed using both in-plane and out-of-plane methods with similar results.
Keywords: Central Venous Catheter, Ultrasound-Guided, Internal Jugular Vein, Oncology -
هدف
آزمایش به منظور بررسی تاثیر نانوذرات (روی و سیلیکون) و پوترسین بر اجزای پرشدن دانه گندم تحت تنش شوری اجرا شد.
روش پژوهش:
آزمایش به صورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی و در سه تکرار در گلخانه تحقیقاتی دانشگاه محقق اردبیلی در سال 1401-1400 اجرا شد. فاکتورهای موردبررسی شامل شوری در چهار سطح (عدم اعمال شوری به عنوان شاهد، اعمال شوری 40، 80 و 120 میلی مولار با نمک کلریدسدیم) و محلول پاشی نانوذرات و پوترسین در هشت سطح (محلول پاشی با آب به عنوان شاهد، محلول پاشی 50 میلی گرم در لیتر نانوسیلیکون، محلول پاشی یک گرم در لیتر نانواکسیدروی، محلول پاشی یک میلی مولار پوترسین، محلول پاشی توام نانوسیلیکون و نانواکسیدروی، محلول پاشی نانوسیلیکون و پوترسین، محلول پاشی نانواکسیدروی و پوترسین، محلول پاشی سیلیکون و نانواکسیدروی با پوترسین) بودند.
یافته هانتایج نشان داد بیش ترین طول دوره و دوره موثر پرشدن دانه در محلول پاشی نانوذرات و پوترسین به دست آمدند. این ترکیب تیماری در شرایط عدم اعمال شوری وزن و حجم ریشه (به ترتیب 1/48 و 03/53 درصد)، شاخص کلروفیل (75/45 درصد) و سرعت پرشدن دانه (20 درصد) را افزایش داد. یک افزایش به ترتیب 34/45، 72/62، 5/40، 21/36 و 34/37 درصدی در ارتفاع بوته، طول سنبله، تعداد دانه در سنبله، وزن صددانه و عملکرد دانه در شرایط عدم اعمال شوری و محلول پاشی نانوذرات و پوترسین نسبت به عدم محلول پاشی تحت شوری 120 میلی مولار وجود داشت.
نتیجه گیریبه نظر می رسد محلول پاشی نانوذرات و پوترسین در شرایط شوری می تواند عملکرد گندم را به واسطه بهبود خصوصیات ریشه و مولفه های پرشدن دانه افزایش دهد.
کلید واژگان: حجم ریشه, سرعت پرشدن دانه, شاخص کلروفیل, طول دوره پرشدن دانه, وزن دانهObjectiveThis experiment was conducted to investigate the effects of nanoparticles (Zn and Si) and putrescine on grain-filling components of wheat under salinity stress.
MethodsAn experimental factorial based on a randomized complete block design with three replications was conducted at the research greenhouse of the University of Mohaghegh Ardabili in 2022. The experimental factors included salinity at four levels (without salinity application as control, application of 40, 80, and 120 mM salinity with NaCl) and nanoparticle and putrescine foliar application at eight levels (foliar application with water as control, foliar application of 50 mg.L-1 of Si, foliar application of 1 g.L-1 of Zn, foliar application of 1 mM putrescine, foliar application of Si-Zn, foliar application of Si and putrescine, foliar application of Zn and putrescine, foliar application of Si-Zn and putrescine).
ResultsThe results showed that the highest grain-filling period and effective grain-filling period were obtained under nanoparticles and putrescine foliar application. This treatment combination under non-saline condition increased the root weight and volume (48.1 and 53.03% respectively), chlorophyll index (45.75%), and grain-filling rate (20%). There was an increase of 45.34, 62.72, 40.5, 36.21 and 37.37% in plant height, spike length, number of grains per spike, 100 grains weight and grain yield, respectively, under non-saline condition and foliar application with nanoparticle and putrescine in compared with no foliar application under 120 mM salinity.
ConclusionIt seems that the foliar application of nanoparticles and putrescine under salinity can increase the yield of wheat by improving root characteristics and grain-filling components.
Keywords: Chlorophyll Index, Grain Filling Period, Grain Filling Rate, Grain Weight, Root Volume -
Background
The objective of this study was to investigate the relationship between the increase in color Doppler ultrasound indices of the ophthalmic artery and the serum lactate level and the severity of the disease in patients with sepsis.
MethodsThis was a cross-sectional study conducted on 24 patients diagnosed with sepsis, who were admitted to the intensive care unit of Shariati Hospital. The patients were more than 18 years old, hospitalized in the first 42 hours, and selected by the portable color Doppler ultrasound available in the ICU department. The ESI probe was placed on the patient’s eye with the eyelid closed, and the ophthalmic artery was located. The indices including RI, PSV, PI were measured along with their serum lactate levels. The correlation of these indices with the severity of sepsis was checked.
ResultsIn this study, 24 patients who were less than 81 years old were diagnosed with sepsis and had a sofa score above 3. It was observed that there is a significant relationship between the dose of increased lactate levels and PSV1, and this parameter can be considered a predictive factor. It was also observed that there is a significant relationship between the dose of increased lactate levels and PSV2, and this parameter can be considered as a predictive factor. Additionally, there is a significant relationship between the dose of increased levels of lactate and PI, and this parameter can be considered as a predictive factor.
ConclusionsThe study found a significant relationship between increased levels of lactate and decreased blood supply in the optic artery. It is suggested that due to the lack of numbers in this study, more samples should be investigated in future studies. In a scientific article, the use of “we” and “our” should be avoided. If necessary, the third person should be used.
Keywords: Septic Shock, Sepsis, Lactate, Optic Nerve Sheath Diameter -
زمینه و هدف
استرس اکسیداتیو می تواند به سلول ها، پروتیین ها و DNA آسیب برساند و در فرایند پیری نقش داشته باشد. این مطالعه به منظور تعیین اثر شش هفته تمرین پیلاتس و مکمل لیسیوم بارباروم بر شاخص های استرس اکسیداتیو بزاق و ترکیب بدن زنان سالمند غیرفعال انجام شد.
روش بررسیاین کارآزمایی میدانی روی 49 زن سالمند غیرفعال انجام شد. آزمودنی ها به صورت هدفمند انتخاب و به صورت تصادفی ساده در 4 گروه شامل کنترل، مکمل، تمرین و تمرین توام با مکمل تقسیم شدند. پروتکل برنامه تمرین طی شش هفته، هر هفته سه جلسه به مدت45 تا60 دقیقه با شدت 50 تا70 درصد حداکثر ضربان قلب بیشینه اجرا شد. گروه مکمل لیسیوم بارباروم به میزان 14 گرم روزانه مصرف کردند. شاخص های استرس اکسیداتیو بزاق و ترکیب بدن مورد مقایسه قرار گرفت. سطح فعالیت جسمانی افراد با استفاده از پرسشنامه ارزیابی فعالیت جسمانی Kaiser (Kaiser physical activity survey) و وضعیت تندرستی نمونه ها با استفاده از پرسشنامه تعیین آمادگی برای فعالیت بدنی (Physical Activity Readiness Questionnaire: PAR-Q) تعیین شد.
یافته هامیزان مالون دی آلدیید در گروه تمرین توام با مصرف مکمل در مقایسه با گروه کنترل، گروه تمرین و گروه مکمل کاهش آماری معنی داری داشت (P<0.05). میزان مالون دی آلدیید در گروه تمرین و گروه مکمل در مقایسه با گروه کنترل از نظر آماری معنی دار نبود. میزان کاتالاز بزاقی در گروه تمرین توام با مصرف مکمل در مقایسه با گروه کنترل، گروه تمرین و گروه مکمل از نظر آماری معنی دار نبود. میزان کاتالاز بزاقی در گروه تمرین در مقایسه با گروه کنترل و نیز در مقایسه با گروه مکمل افزایش آماری معنی داری یافت (P<0.05). میزان گلوتاتیون پراکسیداز در گروه تمرین توام با مصرف مکمل در مقایسه با گروه کنترل تفاوت آماری معنی داری نداشت؛ اما در مقایسه با گروه مکمل افزایش آماری معنی داری یافت (P<0.05). میزان گلوتاتیون پراکسیداز در گروه تمرین در مقایسه با گروه مکمل افزایش آماری معنی داری یافت (P<0.05).
نتیجه گیریتمرین منظم با شدت متوسط و مکمل لیسیوم بارباروم منجربه بهبود عملکرد آنتی اکسیدانی و ترکیب بدن زنان سالمند غیرفعال گردید.
کلید واژگان: تمرین, لیسیوم بارباروم, استرس اکسیداتیو, سالمندیBackground and ObjectiveOxidative stress can harm cells, proteins, and DNA and play a role in the aging process. The present study was conducted to determine the impacts of six weeks of Pilates exercises and Lycium barbarum supplement on oxidative stress indices of saliva and body composition of inactive elderly females.
MethodsThis field trial was carried out on 49 inactive elderly females. The subjects were selected in a purposeful manner and were randomly assigned to 4 groups, including control, supplement, exercise, and exercise plus supplement. The exercise program protocol was implemented for six weeks, three 45-60 minute sessions per week with an intensity of 50-70% maximum heart rate. The Lycium barbarum supplement group used 14 g of supplement daily. Oxidative stress indices of saliva and body composition were compared. The individuals’ physical activity level was determined using the Kaiser Physical Activity Survey (KPAS) and the samples’ health status was specified using the Physical Activity Readiness Questionnaire (PAR-Q).
ResultsThe amount of malondialdehyde had a statistically significant reduction in the exercise plus supplement group compared to the control group, the exercise group, and the supplement group (P<0.05). The amount of malondialdehyde was not statistically significant in the exercise group and the supplement group compared to the control group. The amount of salivary catalase was not statistically significant in the exercise group plus supplement group compared to the control group, the exercise group, and the supplement group. The amount of salivary catalase had a statistically significant increase in the exercise group compared to the control group and the supplement group (P<0.05). The amount of glutathione peroxidase had no statistically significant difference in the exercise plus supplement group compared to the control group but had a statistically significant increase compared to the supplement group (P<0.05). The amount of glutathione peroxidase had a statistically significant increase in the exercise group than in the supplement group (P<0.05).
ConclusionRegular exercise with moderate intensity and Lycium barbarum supplement resulted in improved antioxidant function and body composition in inactive elderly females.
Keywords: Exercise, Lycium barbarum, Oxidative Stress, Aging -
سلولهای بنیادی مزانشیمی (BMSC)، به سلول هایی گویند که توانایی تمایز به دودمان مزودرمی را دارند. استفاده از آن ها نانوکامپوزیتی در ترمیم نقیصه استخوانی استخوان ران موش رت بود. در این مطالعه از 36 سر رت نژاد ویستار نر به طور تصادفی به چهارگروه سه سری در سه روز 15، 30 و 45 تقسیم شدند. گروه های مورد مطالعه شامل شاهد (Sham) : قیصه بدون کارگذاری داربست رها و ناحیه بسته شد، HA : نقیصه با استفاده از هیدروکسی آپاتایت ابتدا پر و سپس بسته شد، اتوگرافت (Autograft) : نقیصه با استفاده از تکه استخوان های ناحیه دریل شده پر شده و بسته شد و بنیادی (MSCs) : مانند گروه شاهد بود ولی تزریق سلول های بنیادی مزانشیمی بصورت موضعی در لحظه جراحی صورت گرفت. به این صورت که یک برش به طول 5 سانتی متر در شرایط آسپتیک در امتداد اندام عقبی سمت راست روی دیافیز میانی استخوان ران انجام شد. پریوستوم توسط یک بلندکننده پریوست از دیافیز جدا شد. یک نقیصه با قطر 2 میلی متر در استخوان ران ایجاد شد. نمونه های استخوان برای ارزیابی هیستوپاتولوژی در روزهای 30 و 45 برای درجه بندی ضایعات و میزان ترمیم، فیکس، دکلسیفیه و عمل آوری شدند. اثرات ترمیمی استفاده از سلول های بنیادی در مقایسه با سایر گروه ها در همه موارد تفاوت معنی داری داشت. می توان اذعان داشت که حضور سلول های بنیادی مزانشیمی تاثیر مثبتی بر روند بهبودی داشته و استحکام استخوانی رضایت بخشی را نشان می دهد. می توان از سلول های بنیادی مزانشیمی به طور گسترده در جراحی ارتوپدی و همچنین مهندسی بافت استفاده کرد.
کلید واژگان: نقیصه, استخوان, ترمیم, سلول بنیادی مزانشیمی, هیدروکسی آپاتیتMesenchymal stem cells (BMSC) can differentiate into the mesodermal lineage. The use of nanocomposite mesenchymal stem cells in the repair of the bone defect of the rat femur. In this study, 36 male Wistar rats were randomly divided into three groups on days 15, 30, and 45. The studied groups include Sham; the defect was left without scaffolding, and the area was closed. HA: The defect was first filled and then closed using hydroxyapatite. Autograft: The defect was filled using pieces of bone from the drilled area and the stem cells (MSCs); that was the same as the control group, but the injection of mesenchymal stem cells was done locally at the time of surgery. In this way, a 5 cm long incision was made in aseptic conditions along the right hind limb on the medial diaphysis of the femur. The periosteum was separated from the diaphysis by a periosteal lifter. A defect with a diameter of 2 mm was created in the femur. Bone samples were fixed, decalcified, and processed for histopathological evaluation on days 30 and 45 to grade lesions and repair rate. The restorative effects of using stem cells were significantly different in all cases compared to other groups. It can be acknowledged that the presence of mesenchymal stem cells positively affects the healing process and shows satisfactory bone strength. Mesenchymal stem cells can be widely used in orthopedic surgery and tissue engineering.
Keywords: Defect, Bone, Healing, Mesenchymal Stem Cell, Hydroxyapatite -
به منظور بررسی تاثیر محلول پاشی نانوذرات (روی و سیلیکون) و پوترسین بر شاخص های فلورسانس کلروفیل، انتقال ماده خشک و سهم این فرآیند در عملکرد دانه گندم تحت تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوک کاملا تصادفی در سه تکرار در گلخانه پژوهشی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 1400 اجرا شد. فاکتورهای مورد بررسی شامل شوری در چهار سطح (عدم اعمال شوری به عنوان شاهد و اعمال شوری 40، 80 و 120 میلی مولار)، با نمک کلرید سدیم و محلول پاشی نانوذرات (روی و سیلیکون) و پوترسین در هشت سطح (محلول پاشی با آب به عنوان شاهد، محلول پاشی 50 میلی گرم درلیتر نانوسیلیکون، محلول پاشی یک گرم در لیتر نانواکسید روی، محلول پاشی یک میلی مولار پوترسین، محلول پاشی نانوسیلیکون و نانواکسید روی، محلول پاشی نانوسیلیکون و پوترسین، محلول پاشی نانو اکسید روی و پوترسین، محلول پاشی همزمان نانواکسید روی و سیلیکون با پوترسین) بودند. نتایج نشان داد که محلول پاشی همزمان نانوذرات و پوترسین در شرایط عدم اعمال شوری، فلورسانس متغیر (8/95 درصد)، فلورسانس حداکثر (33/35 درصد)، عملکرد کوانتومی (24/42 درصد)، شاخص سطح برگ (19/86 درصد)، پروتیین برگ پرچم (8/41 درصد)، ماده خشک کل (09/44 درصد)، فتوسنتز جاری (15/87 درصد) و سهم این فرآیند در عملکرد دانه (04/36 درصد) و عملکرد دانه (34/37 درصد) را نسبت به شرایط عدم محلول پاشی نانوذرات و پوترسین در بالاترین سطح شوری افزایش داد. ولی حداکثر میزان انتقال ماده خشک از ساقه و اندام هوایی (به ترتیب 80/37 و 15/38 درصد) و سهم این فرآیندها در عملکرد دانه (به ترتیب 45/89 و 22/90 درصد) در شرایط عدم محلول پاشی نانوذرات و پوترسین تحت شوری 120 میلی مولار بدست آمد. به نظر می رسد کاربرد نانوذرات و پوترسین با بهبود فتوسنتز جاری و شاخص های فلورسانس کلروفیل، می تواند عملکرد دانه گندم را تحت تنش شوری افزایش دهد.
کلید واژگان: پروتئین برگ, شاخص سطح برگ, عملکرد کوانتومی, فتوسنتز جاری, فلورسانس حداکثرIn order to study the effects of foliar application of nanoparticles (zinc and silicon) and putrescine on chlorophyll fluorescence indices, dry matter remobilization and the contribution of this process in grain yield of wheat under salinity stress, an experimental as factorial based on a randomized complete block design with three replications will be conducted in the research greenhouse of the Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2021. Factors experimentally included salinity at four levels (no salinity as control, salinity 40, 80 and 120 mM by NaCl), and foliar application of nanoparticles (Zn and Si) and putrescine at eight levels (foliar application with water as control, foliar application 50 mg.L-1 nano silicon, foliar application, 1 g.L-1 nano znic oxide, foliar application of putrescine, foliar application of nano Si-Zn oxide, foliar application of nano silicon and putrescine, foliar application of nano Zn oxide and putrescine, foliar application of nano Si-Zn oxide and putrescine). The results showed that foliar application of nanoparticles (Zn and Si) and putrescine under no salinity increased variable fluorescence (95.8%), maximum fluorescence (35.33%), quantum yield (42.24%), leaf area index (45.27%), flag leaf protein (41.8%), total dry matter (44.09%), current photosynthesis (87.15%) and the contribution of current photosynthesis in grain yield (36.04%) and grain yield (37.34 %) compared to no foliar application of nanoparticles and putrescine under the highest salinity level. But the maximum dry matter remobilization from stem and shoot (37.8 and 38.15%, respectively) and the contribution of these processes to grain yield (89.45 and 90.22%, respectively) were obtained in no foliar application of nanoparticles and the putrescine under salinity 120 mM. It seems that nanoparticles and putrescine application can increase grain yield of wheat under salinity stress due to improving current photosynthesis and chlorophyll fluorescence indices.
Keywords: Current photosynthesis, Leaf protein, Leaf area index, Maximum fluorescence, Quantum yield -
Insufficient scholarly inquiry exists regarding the function of genres and their pertinent discourse characteristics within the realm of Information Structure and its syntactic renderings in the Persian language. The present study, which is descriptive and corpus-based in nature, is designed to analyze three distinct genres of the Persian language – specifically, political discourse, scientific discourse, and everyday conversation. The study aims to investigate the Information Structure present in these genres, as expressed through four key syntactic devices: clefting, pseudo-clefting, passive voice, and preposing. In order to achieve the intended objective, one thousand distinct verbal expressions concerning each of the aforementioned genres were obtained from the multi-media database of the IRNA (Iranian News Agency) website and everyday dialogues amongst acquaintances and family members of the researchers, utilizing Lambrecht's theoretical framework for constructing the Information Structure. Following data collection, transcription was carried out and various syntactic devices were identified. Subsequently, descriptive statistics were utilized to analyze and understand the representation of these syntactic devices across different genre types. The findings indicated that there are distinct variations in the depiction of instruments across the three classifications of political discourse, scientific discourse, and commonplace discussions. This discovery may hold significance for scholars of linguistics and language theory.Keywords: information structure, Clefting, genres, passive, Preposing, Pseudo-clefting, Syntactic devices
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به منظور بررسی تاثیر محلول پاشی متانول بر عملکرد، مولفه های پر شدن دانه و انتقال ماده خشک گندم تحت شرایط قطع آبیاری، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوکهای کامل تصادفی با سه تکرار در مزرعه پژوهشی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 98-1397 اجرا شد. فاکتورهای مورد بررسی شامل سطوح آبیاری (آبیاری کامل به عنوان شاهد، قطع آبیاری در50 درصد مراحل سنبلهدهی و آبستنی به ترتیب به عنوان محدودیت ملایم و شدید آبی بر اساس کد 55 و 43 مقیاس BBCH) و محلول پاشی متانول در چهار سطح (محلول پاشی با آب به عنوان شاهد و محلول پاشی 10، 20 و 30 درصد حجمی متانول) بود. نتایج نشان داد بیش ترین سهم انتقال ماده خشک از اندام های هوایی (20.52 درصد) و مشارکت ذخایر ساقه در عملکرد دانه (42/16 درصد) در قطع آبیاری در مرحله آبستنی و عدم محلول پاشی به دست آمد. بیش ترین طول دوره و دوره موثر پرشدن دانه (به ترتیب 33.62 و 29.33 روز) در شرایط آبیاری کامل و در سطوح بالای متانول (30 درصد حجمی) و کم ترین آن در عدم محلول پاشی و تحت شرایط قطع آبیاری در مرحله آبستنی به-دست آمد. همچنین نتایج نشان داد که یک افزایش 46.98، 194.17، 37.54، 69.82 و 15.96 درصدی به ترتیب در عملکرد دانه، شاخص سطح برگ، شاخص کلروفیل، میزان فتوسنتز جاری و سهم این فرآیند در عملکرد دانه در محلول پاشی 30 درصد حجمی متانول در شرایط آبیاری کامل نسبت به عدم محلول پاشی در شرایط قطع آبیاری در مرحله آبستنی وجود داشت. بر اساس نتایج این بررسی به نظر می رسد محلول پاشی متانول می تواند به عنوان یک روش مناسب برای افزایش عملکرد دانه در شرایط محدودیت آبی به کار برده شود.
کلید واژگان: سرعت پرشدن دانه, شاخص سطح برگ, محدودیت آبی, گندمIntroductionIn arid and semi-arid regions, drought stress as the main factor and salinity stress as a secondary factor reduces plant growth and yield. Water limitation can damage pigments and plastids, reduce chlorophyll a, b, rate and grain filling period. One of the important strategies for increasing carbon dioxide concentration in plants is using chemicals such as methanol that can increase the concentration of CO2 in a plant and improves photosynthesis rate and growth under water deficit conditions. Methanol is considered as quasi-essential for plant growth and development, and alleviates toxic effects caused by various environmental stresses in plants. The aim of this study was to investigate the effects of methanol on the agro –physiological traits (i.e., chlorophyll content and grain filling period) and yield of wheat under water limitation condition
Material and methodA factorial experiment was conducted based on randomized complete block design with three replications at the research farm of Faculty of Agriculture and Natural Resources of University of Mohaghegh Ardabili, during 2015-2016. Factors experiment were included irrigation levels (full irrigation as control, irrigation withholding in 50% of booting and heading stages as severe and moderate water limitation respectively, according with 43 and 55 BBCH code) and methanol foliar application at four levels (foliar application with water as control and foliar application 10, 20 and 30% volume of methanol). Foliar application with methanol was done in two stage of period growth (Stem elongation and Flag leaf development).
Results and discussionResults showed that the maximum contribution of dry matter remobilization from air parts (20.52%) and contribution of stem reserves in grain yield (16.42%) were obtained with irrigation withholding at booting stage and no foliar application. The maximum grain filling period and effective grain filling period (33.62 and 29.33 days respectively) were obtained in full irrigation and foliar application of 30% volume of methanol and its minimum was obtained at no foliar application under irrigation withholding in booting stage. Also, results showed that there were an increase about 46.98%, 194.17%, 37.54%, 69.82% and 15.96% in grain yield, leaf area index, chlorophyll index, current photosynthesis and contribution of current photosynthesis in grain yield respectively in full irrigation and foliar application of 30% volume of methanol in comparison with no foliar application of methanol under irrigation withholding in booting stage. Based on the results this study, foliar application of 30% volume of methanol can be applied as a proper method to increase grain yield under water limitation conditions.
Keywords: Grain filling rate, Leaf area index, Methanol, Water limitation, Wheat -
به منظور بررسی تاثیر نانوذرات (نانواکسید آهن و سیلیکون) و کودهای بیولوژیک (آزوسپریلیوم لیپوفروم و سودوموناس پوتیدا) بر اجزای فلورسانس کلروفیل و برخی صفت های فیزیولوژیک تریتیکاله در سطوح مختلف آبیاری، آزمایشی به شکل فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی در سه تکرار اجرا شد. فاکتورهای بررسی شده، سه سطح آبیاری (آبیاری کامل به عنوان شاهد، قطع آبیاری در 50 درصد مراحل چکمه ای شدن (آبستنی) و سنبله دهی به ترتیب به عنوان محدودیت شدید و ملایم آبی)، کاربرد کودهای زیستی (آزوسپریلیوم لیپوفروم و سودوموناس پوتیدا) در چهار سطح و محلول پاشی نانوذرات (نانواکسید آهن و سیلیکون) در چهار سطح بودند. کاربرد توام باکتری های محرک رشد و محلول پاشی نانوذرات در شرایط آبیاری کامل، شاخص کلروفیل (23/50 درصد)، محتوای نسبی آب (97/43 درصد)، هدایت روزنه ای (78/36 درصد)، عملکرد کوانتومی (38/47 درصد)، فلورسانس حداکثر (84/34 درصد)، فلورسانس متغیر (49/98 درصد) و عملکرد دانه (28/43 درصد) را در مقایسه با قطع آبیاری در مرحله آبستنی و به کارنبردن کودهای بیولوژیک و نانوذرات افزایش داد. کاربرد توام نانوذرات و باکتری های محرک رشد، شاخص کلروفیل، محتوای نسبی آب، هدایت روزنه ای، عملکرد کوانتومی، فلورسانس حداکثر، فلورسانس متغیر و عملکرد دانه در هر دو سطح آبیاری کامل و محدودیت شدید آبی را افزایش داد، ولی نشت الکترولیت و فلورسانس حداقل را کاهش بخشید؛ همچنین در شرایط محدودیت شدید آبی یا قطع آبیاری در مرحله آبستنی، کاربرد توام نانوذرات و باکتری های محرک رشد شاخص کلروفیل (74/7 درصد)، محتوای نسبی آب (99/10 درصد)، هدایت روزنه ای (46/22 درصد)، عملکرد کوانتومی (2/15 درصد)، فلورسانس حداکثر (54/26 درصد)، فلورسانس متغیر (61/75 درصد) و عملکرد دانه (59/28 درصد) را نسبت به شرایط به کارنبردن نانوذرات و کودهای بیولوژیک در همین سطح از سطوح آبیاری افزایش داد. به طورکلی، کاربرد توام کودهای بیولوژیک و نانوذرات به واسطه بهبود صفت های فیزیولوژیک گیاه، عملکرد دانه بهتری را در سطوح مختلف آبیاری نشان داد.کلید واژگان: سودوموناس, عملکرد کوانتومی, محتوای نسبی آب, نانوسیلیکون, نشت الکترولیتrlmIn order to investigate the effects of nanoparticles) nano Fe-Si oxide) and biofertilizers (Azospirillum lipoferum and Pseudomonas putida) on chlorophyll fluorescence components and some physiological traits of triticale at different levels of irrigation, an experimental as the factorial study was conducted based on randomized complete block design with three replications. The studied factors included three levels of irrigation (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe, and moderate water limitation, respectively), application of biofertilizers (Azospirillum lipoferum and Pseudomonas putida) in four levels, and nanoparticles foliar application (nano iron oxide and nano silicon oxide) at four levels. Both applications of plant growth-promoting rhizobacteria and nanoparticle foliar application under full irrigation increased chlorophyll index (50.23%), relative water content (43.97%), stomatal conductance (36.78%), quantum yield (47.38%), maximum fluorescence (34.84%), variable fluorescence (98.49%), and grain yield (43.28%) in comparison to no application of biofertilizers and nanoparticles under irrigation withholding at booting stage. Both applications of plant growth-promoting rhizobacteria and nanoparticles increased the chlorophyll index, relative water content, stomatal conductance, quantum yield, maximum fluorescence, variable fluorescence and grain yield in both levels of full irrigation and severe water limitation, but decreased electrolyte leakage and minimum fluorescence. In addition, severe water limitation or irrigation withholding at the booting stage increased chlorophyll index (7.74%), relative water content (10.99%), stomatal conductance (22.46%), quantum yield (15.2%), maximum fluorescence (26.54%), variable fluorescence (75.61%) and grain yield (28.59%) in comparison to no application of biofertilizers and nanoparticles at the same irrigation level. Generally, the application of biofertilizers and nanoparticles showed a better performance at different irrigation levels due to the improvement of physiological traits.IntroductionTriticale is a human-made crop, being a hybrid by cross-fertilization of wheat (Triticum spp.) and rye (Secale spp.). In general, triticale combines the high yield potential of wheat with the biotic and abiotic stress tolerance of rye, making it more suitable for production in marginal areas (acidic, saline, or soils with heavy metal toxicity) (Cantale et al. 2016). In arid and semi-arid regions, drought stress as the main factor and salinity stress as a secondary factor decrease plant growth and yield. Water limitation can damage pigments and plastids, and educe chlorophyll index, stomatal conductance, quantum yield, and relative water content. Several strategies have been developed in order to decrease the toxic effects caused by environmental stresses on plant growth. Among them, the use of bio-fertilizers such as plant growth-promoting rhizobacteria (PGPR) and also nanoparticles such as nano iron-silicon oxide plays a very important role in yield improvement. Inoculation of plants with native suitable microorganisms may decrease the deleterious effects of environmental stresses and increase stress resistance of plants by various mechanisms, including synthesis of phytohormones such as auxins, cytokinin and gibberellins, solubilization of minerals like phosphorus, production of siderophores and increase in nutrient uptake. There is little information on the effects of nanoparticles and biofertilizers on chlorophyll fluorescence components and some physiological traits of triticale at different irrigation levels. Therefore, the objective of the present study was to evaluate the effect of nanoparticles (nano Fe-Si oxide) and biofertilizers (Azospirillum lipoferum and Pseudomonas putida) on chlorophyll fluorescence components and some physiological traits of triticale at different levels of irrigation.
Materials and MethodsAn experiment as factorial was conducted based on a randomized complete block design with three replications at the research farm of the Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2021. The experimental factors included irrigation in three levels (full irrigation as control, irrigation withholding at 50% of booting and heading stages as severe, and moderate water limitation, respectively (BBCH 43 and 55 respectively), the application of biofertilizers in four levels (no application as control, application of Azospirillum, Pseudomonas, both application Azospirillum + Pseudomonas) and nanoparticles foliar application at four levels (foliar application with water as control, nano iron oxide foliar application (1 g.L-1), nano silicon oxide (50 mg.L-1), both application nano iron-silicon oxide). Psedomunas and Azospirillum were isolated from the rhizospheres of wheat by the Research Institute of Soil and Water, Tehran, Iran. For inoculation, seeds were coated with gum Arabic as an adhesive and rolled into the suspension of bacteria until uniformly coated. The strains and cell densities of microorganisms used as PGPR in this experiment were 1×108 colony forming units (CFU). In each plot, there were 5 rows with 2 m long. In each experimental plot, two beside rows and 0.5 m from the beginning and end of planting lines were removed as margin, and measurements were done on 0.2 m2 for grain yield. The used nano silicon-iron oxide had an average particle size of less than 30 nm and the special surface of particles was more than 30 m2.g-1. They were the product of Nanomaterial US Research which was provided by Pishgaman Nanomaterials Company of Iran. Nano silicon oxide and nano silicon oxide powder were added to deionized water and placed on ultrasonic equipment (100 W and 40 kHz) on a shaker for better solution. Foliar application of nano silicon and nano iron oxide was done in two stages of period growth BBCH 21 and 30. Chlorophyll Index was calculated by a chlorophyll meter (SPAD-502; Konica Minolta Sensing, Inc., Japan). RWC and EL were measured according to the method of Kostopoulou et al. (2010) and Farooq and Azam (2006), respectively. Quantum yield was measured on flag leaves by the uppermost fool expanded leaf using a fluorometer (chlorophyll fluorometer; Optic Science-OS-30 U.S.A.). Analysis of variance and mean comparisons were performed using software package SAS v9.12. The main effects and interactions were compared by LSD (least significant difference) test at the 0.05 probability level, using the SAS version 9.1.
Results and DiscussionThe results showed that both applications of plant growth-promoting rhizobacteria and nanoparticle foliar application under full irrigation increased chlorophyll index (50.23%), relative water content (43.97%), stomatal conductance (36.78%), quantum yield (47.38%), maximum fluorescence (34.84%), variable fluorescence (98.49%), and grain yield (43.28%) in comparison to no application of biofertilizers and nanoparticles under irrigation withholding at booting stage. Both applications of plant growth-promoting rhizobacteria and nanoparticles increased chlorophyll index, relative water content, stomatal conductance, quantum yield, maximum fluorescence, variable fluorescence, and grain yield at both levels of full irrigation and severe water limitation, but decreased electrolyte leakage and minimum fluorescence. In addition, severe water limitation or irrigation withholding at the booting stage increased chlorophyll index (7.74%), relative water content (10.99%), stomatal conductance (22.46%), quantum yield (15.2%), maximum fluorescence (26.54%), variable fluorescence (75.61%), and grain yield (28.59%) in comparison to no application of biofertilizers and nanoparticles at the same level from irrigation levels.
ConclusionBased on the results of the study, the application of biofertilizers and nanoparticles showed a better performance at different irrigation levels due to the improvement of physiological traits. In other words, the effects of the simultaneous application of PGPR and nanoparticle inoculation are more positive than the single application of PGPB and nanoparticles. Therefore, one of the suitable methods to improve plants' resistance to environmental stress is the application of PGPB and nanoparticles, which play a very important role in yield and growth improvement.Keywords: Pseudomonas, Quantum yield, Relative water content, Nano silicon, Electrolyte leakage -
Journal of Dentistry, Shiraz University of Medical Sciences, Volume:23 Issue: 3, Sep 2022, PP 361 -368
Statement of the Problem:
Oral lichen planus (OLP) is an important inflammatory disease concerning its tendency to malignancy. The etiopathogenesis of this disease is still unknown. Medical geography uses geographic techniques to study factors related to location that cause uneven distribution of disease.
PurposeThis study was conducted to map OLP in patients referred to Shiraz Dental School with medical geography techniques and investigate any possible relationship between the number of dentists and health-centers in different regions of Fars province with the number of referrals.
Materials and MethodIn this cross-sectional study, we evaluated the records of OLP patients referred to Shiraz Dental School from 2007 to 2018. Age, sex, place of residence, occupation, level of education, location of involvement, duration, and cutaneous involvement were recorded. The number of dentists and healthcare centers was obtained from the statistical records of Fars province. A Geographic Information System was used for the assessment of the spatial distribution of OLP. Ordinary least squares (OLS) and geographically weighted regression (GWR) indices were used for evaluating the relationship between the number of dentists and health centers with the number of referrals.
ResultsFrom 1006 records, 457 were studied, from which 71% were female (age range of 41-60). The regression coefficient was 0.937 for the number of dentists in each county compared to OLP referrals indicating a strong relationship. The regression coefficient was 0.983 for the number of health-centers. According to GWR analyses in Shiraz neighboring counties like Marvdasht, Sepidan, and Sarvestan, there is a positive relationship between the number of dentists and OLP patients.
ConclusionThe results revealed that dentists and health care centers had a good cognition of referring patients with OLP (as a premalignant lesion) for definite diagnosis in Fars counties. But both groups need more education in this matter. Moreover, referrals from settlements near Shiraz were more common because of their easier access.
Keywords: Medical Geography, Dentists, Spatial Distribution, Oral lichen planus -
درتحقیق پیکره-محو حاضرنقش گونه های زبانی و ویژه گی های گفتمانی ساخت اطلاع و نمود نحوی آن در زبان فارسی بررسی می شود. به بیانی دیگر، در این تحقیق مطالعه تجلی گونه های سیاسی، علمی و محاوره ای در قالب ابزارهای نحوی مختلف مورد نظراست. بدین منظور، بر اساس چارچوب نظری "لمبرشت" تعداد هزار عبارت از هر یک از این سه گونه زبانی از منابع مختلف جمع آوری شده، ساختارهای دستوری فراوان-کاربرد هر یک شناسایی گردید. سپس از طریق آزمون "من وایتلی یو" مشخص گردید کدام ابزار نحوی ساخت اطلاع در این سه گونه بیشترین کاربرد را دارد. تحلیل داده ها نشان داد تفاوت معنا داری بین این گونه ها از نظر فراوانی کاربرد ابزار نحوی وجود دارد. در واقع، معلوم گردید ابزارنحوی "پیش آیند ساز"، "مجهول" و "اسنادی" بیشترین کاربرد را بترتیب درگونه های محاوره ای، علمی و سیاسی دارند. این یافته برای متخصصین زبانشاسی، آموزش زبان و نظریه پردازان زبان قابل استفاده خواهد بود.
کلید واژگان: گونه های زبانی, ویژه گی های گفتمانی, ساخت اطلاع, ابزار نحوی, پیش آیند ساز, مجهول, اسنادیThis descriptive and corpus-based study examined the role of genres and their discourse features in information structure and its syntactic representations in Persian. It aimed to investigate three genres of Persian spoken language, that is, political, scientific, and everyday conversation, in terms of their information structure as realized through four syntactic devices of clefting, pseudo-clefting, passive, and preposing. For this purpose, 1000 utterances related to each of the mentioned genres were extracted based on Lambrecht’s theoretical framework for modeling the information structure. The collected data were then transcribed, and the most frequently-used syntactic structures of information structure in each genre were identified. Finally, the Mann-Whitney U test and descriptive statistics were used to see which of the three genre types has higher values than the other two in terms of the four syntactic devices. The results of data analysis revealed that there is a significant difference among the three genres in terms of the frequency of use of syntactic devices. In fact, preposing occurred most frequently in everyday conversations, passives in scientific texts, and clefting and pseudo-clefts in political texts. This finding has supportive implications for linguistic and educational experts as well as language theoreticians.
Keywords: Clefting, Genres, Information structure, Passive, Preposing, Pseudo-clefting, Syntactic devices -
به منظور بررسی تاثیر محلول پاشی و مصرف خاکی روی بر وزن دانه و برخی صفات بیوشیمیایی گندم (Triticum aestivum L.) در شرایط شوری خاک، آزمایشی به صورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی در سه تکرار در گلخانه پژوهشی دانشگاه محقق اردبیلی در سال 98-1397 اجرا شد. فاکتورهای موردبررسی شامل سطوح شوری خاک (سطح شاهد و اعمال شوری های 30، 60 و 90 میلی مولار در خاک) و چهار روش کاربرد روی (شاهد یا عدم کاربرد روی، مصرف خاکی سولفات روی، محلول پاشی نانواکسیدروی و مصرف خاکی و محلول پاشی روی) بود. نتایج نشان داد که کاربرد هم زمان مصرف خاکی سولفات روی و محلول پاشی نانواکسیدروی در شوری 90 میلی مولار خاک، به طور معنی داری فعالیت آنزیم های کاتالاز، پراکسیداز، محتوای آنتوسیانین، پرولین و قندهای محلول را به ترتیب 24/20، 68/17، 16/13، 88/32 و 08/14 درصد نسبت به عدم کاربرد روی در شرایط شوری 90 میلی مولار خاک افزایش داد. هم چنین، کاربرد هم زمان مصرف خاکی سولفات روی و محلول پاشی نانواکسیدروی در شرایط عدم اعمال شوری، محتوای پراکسید هیدروژن و مالون دی آلدهید در مقایسه با عدم کاربرد روی در بالاترین سطح از شوری خاک کاهش داد. کاربرد هم زمان مصرف خاکی سولفات روی و محلول پاشی نانواکسیدروی تحت شرایط عدم اعمال شوری دارای بیش ترین وزن دانه (016/1 گرم در بوته) نسبت به کاربرد این ترکیب تیماری در سایر سطوح شوری بود. به نظر می رسد که کاربرد هم زمان مصرف خاکی سولفات روی و محلول پاشی نانواکسیدروی می تواند وزن دانه ی گندم در شرایط شوری را، به دلیل بهبود صفات بیوشیمیایی افزایش دهد.کلید واژگان: پرولین, قندهای محلول, کاتالاز, مالون دی آلدهید, نانواکسید رویIn order to study the effect of foliar and soil application of Zinc on grain weight and some biochemical traits of wheat (Triticum aestivum L.) under soil salinity, an experiment has been conducted as factorial based on randomized complete block design with three replications in research greenhouse of University of Mohaghegh Ardabili in 2018-2019. Experimental factors include soil salinity levels [control and salinity of 30, 60, and 90 mM] and four methods of zinc application [no zinc as control, soil application zinc as ZnSO4, foliar application nano zinc oxide, and combination of soil and foliar application of zinc]. Results show that both application of ZnSo4 and foliar application nano Zn oxide under 90 mM soil salinity condition increase the catalase and peroxidase enzymes activity, anthocyanin, proline, and soluble sugars content by 20.24%, 17.68%, 13.16%, 32.88%, and 14.08%, respectively, in comparison with no application of zinc under 90 mM soil salinity condition. Also, both soil application of ZnSo4 and foliar application of nano Zn oxide under non-salinity condition decrease hydrogen peroxide and malondialdehyde content in comparison with no application of Zinc under 90 mM soil salinity. Both soil application ZnSo4 and foliar application nano Zn oxide under non-salinity condition has had the highest grain weight (1.016 g per plant), compared to the application of this treatment combination at other salinity levels. It seems that both application of ZnSo4 and nano Zn oxide can increase weight yield of wheat under salinity condition due to their ability in improving biochemical traits.Keywords: Catalase, Malondialdehyde, Nano Zn oxide, Proline, soluble sugars
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In this paper, using the chemical bath deposition method and employing the suitable deposition temperature, internal doping of Cu2+ ions into nanocrystalline ZnS thin films is reported. X-ray diffraction analysis (XRD) was used to evaluate the structure of the films, which consists of sub-7 nm crystallites of the cubic ZnS. FE-SEM images indicated that the surface morphology of the Cu-doped ZnS films is strongly dependent on dopant level. The influence of the Cu2+ ions concentration on optical and specially photoluminescence properties of the Cu:ZnS thin films have been investigated and discussed. Defect-free emission spectra at low deposition temperatures and the appearance of the dopant-related emission peak at higher temperatures demonstrate that this method is an effective strategy for chemical deposition of the Cu-doped ZnS nanocrystals on glass substrates. Furthermore, the concentration quenching effect on photoluminescence intensity has been observed which can be related to non-radiative transitions between electronic energy levels of the neighboring Cu2+ ions in the ZnS host lattice.Keywords: Cu-doped ZnS thin films, Dopant emission, nanocrystals, Photoluminescence
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ببه منظور بررسی تاثیر یونیکونازول و کودهای بیولوژیک بر عملکرد، شاخص های فلورسانس کلروفیل و برخی صفات فیزیولوژیک گندم تحت شرایط شوری خاک، آزمایش بصورت فاکتوریل بر پایه طرح بلوک های کامل تصادفی در سه تکرار در سال 1397 در گلخانه پژوهشی دانشگاه محقق اردبیلی انجام شد. تیمارها شامل شوری خاک در چهار سطح (عدم اعمال شوری به عنوان شاهد یا شوری 72/1 دسی زیمنس بر متر و اعمال شوری های 40، 80 و 120 میلی مولار در خاک به ترتیب معادل 68/3، 37/7 و 06/11 دسی زیمنس بر متر) و کاربرد یونیکونازول و کودهای زیستی در هفت سطح (قارچ میکوریز، یونیکونازول، باکتری سودوموناس، میکوریز با سودوموناس، میکوریز با یونیکونازول، کاربرد میکوریز با یونیکونازول و سودوموناس و شاهد) بود. مقایسه میانگین ها نشان داد که کاربرد توام میکوریز با سودوموناس و یونیکونازول در شرایط عدم اعمال شوری، فلورسانس بیشینه (17/82 %)، فلورسانس متغیر (5/287 %)، عملکرد کوانتومی (41/65 %)، شاخص کلروفیل (23/106 %)، میزان نیتروژن برگ (66/76 %) و محتوای آب نسبی برگ پرچم (76/101 %) را در مقایسه با شوری 120 میلی مولار خاک و عدم کاربرد یونیکونازول و کودهای زیستی افزایش داد. بیشترین هدایت الکتریکی و فلورسانس حداقل (Fo) در بالاترین سطح شوری بدست آمد. همچنین کاربرد توام میکوریز با سودوموناس و یونیکونازول در شرایط عدم اعمال شوری عملکرد تک بوته نسبت به شرایط عدم کاربرد کودهای زیستی و یونیکونازول و در بالاترین سطح از شوری خاک حدود 84/108 درصد افزایش داد. براساس نتایج این بررسی، به نظر می رسد کاربرد کودهای زیستی و یونیکونازول می تواند در بهبود عملکرد گندم در شرایط شوری خاک پیشنهاد شود.کلید واژگان: سودوموناس, شاخص کلروفیل, هدایت الکتریکی, میکوریزIn order to study the effect of uniconazole and bio fertilizers on grain yield, chlorophyll fluorescence indices and some physiological traits of wheat under salinity soil conditions, a factorial experiment was conducted based on RCBD with three replications in 2018. Treatments were included soil salinity in four levels (non-application of salinity as control (1.72 dS m−1 and application of 40, 80 and 120 mM salinity in soil or equivalent to 3.68, 7.37 and 11.06 dS m−1, respectively), by NaCl and application of uniconazole and bio fertilizers in seven levels (control or no application of bio fertilizers and uniconazole, application of uniconazole, mycorrhiza fungi, pseudomonas putida, mycorrhiza with pseudomonas, mycorrhiza with uniconazole, both application of mycorrhiza with uniconazole and pseudomonas). Means comparision showed that both application of mycorrhiza with uniconazole and pseudomonas under no salinity condition increased maximum fluorescence (82.17 %), variable fluorescence (287.5 %), quantum yield (65.41 %), chlorophyll index (106.23 %) , leaf nitrogen content (76.66 %) and relative water content of flag leaf (101.76 %) in comparison with no application of bio fertilizers and uniconazole under 120 mM salinity condition. Maximum of minimum fluorescence (F0) and electrical conductivity were obtained at the highest soil salinity level. Also, both application mycorrhiza with pseudomonas and uniconazole increased grain yield per plant about 108.84% in comparison with no application of bio fertilizers at the highest soil salinity level.Keywords: Chlorophyll index, Electrical Conductivity, Mycorrhiza, Peudomonas
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مقدمه
داشتن فرهنگ مناسب ایمنی به منظور حفظ و ارتقاء ایمنی در سازمان ها ضروری است. ثبت، گزارش دهی و بررسی مناسب رویدادهای شغلی یکی از معیارهای مهم فرهنگ ایمنی است تا بدین وسیله فرهنگ یادگیری مناسبی پس از وقوع رویدادهای شغلی شکل بگیرد. لذا این مطالعه باهدف بررسی فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی در صنایع استان آذربایجان غربی انجام شد.
روش کاردر این مطالعه مقطعی، داده های فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی با استفاده از یک پرسشنامه دارای 68 گویه جمع آوری گردید. تعداد 420 نفر از کارکنان شاغل در تعدادی از شرکت های منتخب ساخت وساز، معدنی و بیمارستان ها در این مطالعه شرکت داشتند.
یافته هانتایج مطالعه نشان داد که میانگین کل نمره فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی (0/38±3/08) بوده و فاکتور اقدامات اصلاحی دارای بیشترین نمره (0/72±3/17) و علل عدم گزارش دهی دارای کمترین نمره (0/54±2/90) بود. همچنین بین متغیر میانگین نمره فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی با تحصیلات، صنایع و شرکت های مختلف ارتباط معنی داری وجود داشت. میانگین نمره فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی کارکنانی که در دوره های آموزشی مشارکت داشتند کمتر از کارکنانی بود که در دوره های آموزشی مشارکت نداشتند. میانگین نمره فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی کارکنانی که تجربه حادثه شغلی داشتند کمتر از کارکنانی بود که تجربه حادثه شغلی درگذشته نداشتند.
نتیجه گیرییافته های این مطالعه نشان داد که انجام اقدامات اصلاحی پس از وقوع رویدادهای شغلی ضروری است و شرکت در دوره های آموزشی و تجربه حادثه شغلی، تاثیر مثبتی در ارتقا فرهنگ ثبت، گزارش دهی و بررسی رویدادهای شغلی نداشته است.
کلید واژگان: حوادث شغلی, آموزش, معدن, ساخت وساز, بیمارستانIntroductionMaintaining and improving organizational safety requires a strong safety culture. Following the occurrence of occupational incidents, proper registration, reporting, and investigation is a key requirement for safety culture to provide an appropriate learning culture. As a result, this study was carried out to assess the culture of occupational incident registration, reporting, and investigation in the province of west Azarbaijan’s industries.
Material and MethodsIn this cross-sectional study, data on the culture of registration, reporting, and investigating occupational incident were collected using a 68-item questionnaire. A total of 420 employees from a number of construction and mine companies, as well as hospitals participated in this study.
ResultsThe results of study showed that the average total score for the culture of registration, reporting, and investigating occupational incidents was 3.08 (±0.38), with the factor of corrective action had the highest 3.17 (±0.72) and the reasons for lack of reporting had the lowest 2.90 (±0.54) scores. There was also a significant relationship between the average score of the culture of registration, reporting, and investigating occupational incidents with education, industries, and companies. Employees who attended training courses had a lower mean score for the culture of occupational incident’ registration, reporting, and investigation than those who did not take training courses. The mean score of the culture of registration, reporting, and investigating occupational incidents of the employees who had experienced an occupational accident was lower than those who had not experience occupational accidents in the past.
ConclusionThe findings of this study revealed that corrective actions is necessary after the occurrence of occupational incidents. Attending training courses and having an occupational accident experience had no positive impact on promoting culture of registration, reporting, and investigating occupational incidents.
Keywords: Occupational accidents, training, mine, construction, hospital -
International Journal of Foreign Language Teaching and Research, Volume:9 Issue: 39, Winter 2021, PP 45 -56There is scarce systematic research on the role of genres and their associated discourse features in Information Structure and its syntactic representations in Persian. The current descriptive, corpus-based study, therefore, aimed to investigate three different genres of Persian spoken language; that is, political, scientific and daily conversation, in terms of their Information Structure as realized through four syntactic devices of clefting, pseudo-clefting, passive and preposing. To this purpose, 1000 different utterances related to each of the mentioned genres, were extracted from the multi-media archive of IRNA (Iranian News Agency) website and daily conversations of the researchers’ family members and friends, based on Lambrecht’s theoretical framework in modeling the Information Structure. The collected data were then transcribed, the syntactic devices were identified, and descriptive statistics were used to see how the above syntactic devices are represented in each genre type. The results revealed that the devices are represented differently in the three genres of political, scientific, and daily conversations. This finding may be of interest to linguists and language theoreticians.Keywords: information structure, Clefting, genres, passive, Preposing, Pseudo-clefting, Syntactic devices
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به منظور ارزیابی عملکرد و برخی صفات بیوشیمیایی گندم تحت تنش شوری خاک در واکنش به کاربرد یونیکونازول و کودهای زیستی، آزمایش فاکتوریلی بر پایه طرح بلوک های کامل تصادفی در سه تکرار در سال زراعی 1397 در گلخانه پژوهشی دانشگاه محقق اردبیلی انجام شد. تیمارها شوری خاک در چهار سطح (عدم اعمال شوری به عنوان شاهد و اعمال شوری های 40، 80 و 120 میلی مولار در خاک) با نمک NaCl و کاربرد یونیکونازول و کودهای زیستی (شاهد یا عدم کاربرد کودهای زیستی و یونیکونازول، کاربرد قارچ میکوریزا، یونیکونازول، باکتری سودوموناس، میکوریز با سودوموناس، میکوریزا با یونیکونازول، کاربرد میکوریزا با یونیکونازول و سودوموناس شاهد یا عدم کاربرد کودهای زیستی و یونیکونازول) را شامل می شدند. نتایج نشان داد کاربرد کودهای زیستی و یونیکونازول تحت شرایط عدم اعمال شوری، محتوای پراکسید هیدروژن، مالون دی آلدهید و هدایت اکتریکی برگ پرچم (به ترتیب به میزان 77.6، 115.52 و 241.48 درصد) نسبت به شرایط عدم کاربرد کودهای زیستی تحت شرایط شوری120میلی مولار خاک کاهش داد ولی شاخص کلروفیل، محتوای نسبی آب، عملکرد کوانتومی، وزن صد دانه و طول سنبله (به ترتیب به میزان 60.21، 43.27، 30.47، 46.66 و 51.34 درصد) نسبت به شرایط عدم کاربرد کودهای زیستی تحت شرایط شوری 120 میلی مولار خاک افزایش داد. همچنین کاربرد کودهای زیستی و یونیکونازول عملکرد دانه تک بوته را نسبت به شرایط عدم کاربرد کودهای زیستی در بالاترین سطح از شوری خاک حدود 108.84 درصد افزایش داد. براساس نتایج این بررسی به نظر می رسد کاربرد کودهای زیستی و یونیکونازول می تواند در بهبود عملکرد گندم در شرایط شوری خاک پیشنهاد شود.
کلید واژگان: پراکسید هیدروژن, عملکرد کوانتومی, مالون دی آلدهید, میکوریزاIntroductionSoil salinity is one of the most serious limiting factors for crop growth and production in the arid and semi-arid regions due to increasing use of poor quality of irrigation water. Several strategies have been developed in order to decrease the toxic effects caused by high salinity on plant growth. Among them, use of bio-fertilizers such as plant growth promoting rhizobacteria (PGPR) and mycorhyza and also plant growth regulator such as uniconazole play a very important role in yield improvement. Inoculation of plants with native suitable microorganisms may decrease the deleterious effects of environmental stresses and increase stress tolerance of plants by a variety of mechanisms, including synthesis of phytohormones such as auxins, cytokinin and gibberellins, solubilization of minerals like phosphorus, production of siderophores and increase in nutrient uptake, N2 fixation. Arbuscular mycorrhizal fungi symbiosis is considered a valuable component in most agricultural systems due to their role in plant nutrition and soil health. So, application of bio fertilizers and uniconazole can improve crop yield under soil salinity stress. Therefore, the aim of this study was evaluation of yield and some biochemical traits of wheat under soil salinity stress in response to uniconazole and bio fertilizers application.
Material and methodsIn order to evaluation of yield and some biochemical traits of wheat under soil salinity stress in response to uniconazole and bio fertilizers application, a factorial experiment was conducted based on randomized complete block design with three replications in a research greenhouse of the Faculty of Agriculture and Natural Resources of Mohaghegh Ardebili University in 2018. Treatments were included soil salinity in four levels (no application of salinity as control and application of 40, 80 and 120 mM soil salinity), by NaCl and application of uniconazole and bio fertilizers (control or no application of bio fertilizers and uniconazole, application of uniconazole, mycorrhiza fungi, pseudomonas putida, mycorrhiza with pseudomonas, mycorrhiza with uniconazole, both application of mycorrhiza with uniconazole and pseudomonas).Mycorrhiza fungi (mosseae) was purchased from the Zist Fanavar Turan corporation and soils were treated based on method of Gianinazzi et al. (2001). Psedomunas putida strain 186 was isolated from the rhizospheres of wheat by Research Institute of Soil and Water, Tehran, Iran. The soil was with pH about 7.8 and EC about 2.68 dsm-1. The wheat cultivar "zagros" was used in the experiment with plant density of 360 seeds m-2. For inoculation, seeds were coated with gum Arabic as an adhesive and rolled into the suspension of bacteria until uniformly coated. The strains and cell densities of microorganisms used as PGPR in this experiment were 1×107 colony forming units (CFU).Relative water content: Weight of fresh leaf was measured just after detached from the plants then taken turgid weight after leaf was incubated in distilled water for 24 h to obtain a full turgidity. Dry weight of leaf was measured after it was dried at 60°C for 24 h in an oven. Relative water content was measured according to the following formula (Chelah et al. 2011). RWC (%) = [(FW-DW) / (TW-DW)] × 100Where, RWC, FW, DW and TW are relative water content, fresh weight, dry weight and turgid weight respectively.Chlorophyll content: A portable chlorophyll meter (SPAD-502; Konica Minolta Sensing, Inc., Japan) was used to measure the leaf greenness of the wheat plants. Quantum yield: The quantum yield was measured by the uppermost fool expanded leaf using a fluorometer (chlorophyll fluorometer; Optic Science-OS-30 USA).Electrical conductivity: Electrical conductivity was calculated by following the standard method of Jodeh et al. (2015). Electrical conductivity (EC) values were measured at room temperature of 23±1 °C using an electrical-conductivity meter. At plant maturity, grain yield in each pot were harvested five plants per pot.Statistical analysis: Analysis of variance and mean comparisons were performed using SAS ver 9.1 computer software packages. The main effects and interactions were tested using the least significant difference (LSD) test at the 0.05 probability level.
Results and discussionThe results showed that application of biofertilizers and uniconazole under no salinity condition decreased hydrogen peroxide content, malondialdehyde and electrobc conductivity of flag leaf (77.6, 115.52 and 241.48% respectively) in comparison with no application of bio fertilizers under 120 mM salinity. But, increased chlorophyll index, relative water content, quantum yield, grain 100 weight and spike length (60.21, 43.27, 30.47,46.66 and 51.34% respectively) in comparison with no application of bio fertilizers under 120 mM salinity condition. Also, application bio fertilizers and uniconazole increased grain yield per plant about 108.84% in comparison with no application of bio fertilizers at the highest soil salinity level. Based on the results of this study, it seems that bio fertilizers and uniconazole application can be suggested to improve of grain yield of wheat under soil salinity condition.
Keywords: Hydrogen peroxide, Malondialdehyde, Mycorrhiza, Quantum yield -
سابقه و هدف :
خشکی از عوامل مهم تاثیرگذار بر عملکرد گیاهان زراعی به خصوص در مناطق خشک و نیمه خشک است. این تنش واکنش های مختلف بیوشیمیایی و فیزیولوژیکی در گیاهان را به عنوان یک سازوکار بقا، القا می کند. خشکی بیش از هر فاکتور محیطی دیگر تولید و عملکرد گیاهان زراعی را محدود می کند. همچنین این تنش می تواند به فتوسنتز گیاهان زراعی صدمه زند و محتوای کلروفیل، محتوای نسبی آب، هدایت روزنه ای و عملکرد کوانتومی را کاهش دهد. یکی از راه کارهای مهم برای افزایش غلظت CO2 در گیاهان استفاده از ترکیباتی همچون متانول است که می تواند غلظت CO2 در گیاه را افزایش دهد و به فتوسنتز و رشد تحت شرایط محدودیت آبی کمک کند. از این رو هدف از این پژوهش، بررسی تاثیر متانول بر عملکرد، روند تغییرات شاخص های فلورسانس کلروفیل و برخی صفات فیزیولوژیک گندم در شرایط قطع آبیاری بود.
مواد و روش هاآزمایش به صورت فاکتوریل در قالب طرح پایه بلوکهای کامل تصادفی با سه تکرار در مزرعه پژوهشی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 98-1397 اجرا شد. فاکتورهای مورد بررسی شامل سطوح آبیاری (آبیاری کامل به عنوان شاهد، قطع آبیاری در 50 درصد مراحل سنبلهدهی و آبستنی به ترتیب به عنوان محدودیت ملایم و شدید آبی بر اساس کد 55 و 43 مقیاس BBCH) و محلول پاشی متانول در چهار سطح (محلول پاشی با آب به عنوان شاهد و محلول پاشی 10، 20 و 30 درصد حجمی متانول) بود.
یافته هانتایج نشان داد قطع آبیاری عملکرد کوانتومی، فلورسانس بیشینه (Fm)، فلورسانس متغیر (Fv)، شاخص کلروفیل، محتوای آب نسبی برگ، هدایت روزنه ای و عملکرد دانه را کاهش داد در حالی که هدایت الکتریکی، نشت الکترولیت و فلورسانس حداقل (F0) افزایش یافت. محلول پاشی 30 درصد حجمی متانول در شرایط آبیاری کامل فلورسانس بیشینه (Fm)، فلورسانس متغیر (Fv)، عملکرد کوانتومی (Fv/Fm)، شاخص کلروفیل، محتوای آب نسبی برگ، هدایت روزنه ای و عملکرد دانه را به ترتیب حدود 53/51، 75/170، 76/78، 69/55، 58/77، 40/79 و 98/46 درصد نسبت به عدم محلول پاشی متانول تحت شرایط قطع آبیاری در مرحله آبستنی افزایش داد. همچنین محلول پاشی با 30 درصد حجمی متانول در شرایط آبیاری کامل هدایت الکتریکی، نشت الکترولیت و فلورسانس حداقل (F0) را به ترتیب 86/122، 70/73 و 59/42 درصد نسبت به عدم محلول پاشی تحت شرایط قطع آبیاری در مرحله آبستنی کاهش داد.
نتیجه گیریبراساس نتایج این بررسی به نظر می رسد محلول پاشی 30 درصد حجمی متانول می تواند در شرایط محدودیت آبی را به واسطه ی بهبود شاخص های فلورسانس و برخی صفات فیزیولوژیکی نظیر هدایت روزنه ای، محتوای نسبی آب و شاخص کلروفیل عملکرد دانه گندم را افزایش دهد.
کلید واژگان: تنش خشکی, نشت الکترولیت, هدایت روزنه ای, محتوای آب نسبیIntroduction:
Drought stress is the most influential factors affecting crop yield particularly in arid and semiarid regions. This stress induces various biochemical and physiological responses in plants as a survival mechanism. Drought limits plant production and the performance of crop plants, more than any other environmental factor. Also this stress can damage the photosynthesis of plants and reduce chlorophyll content, relative water content, stomatal conductance and quantum yield. One of the important strategies for increasing of carbon dioxide concentration in plants is using compounds such as methanol that can increase the concentration of CO2 in a plant and help photosynthesis rate and growth under water deficit conditions. The aim of this study was to investigate the effects methanol on yield, chlorophyll fluorescence indices and some physiological traits of wheat (Triticum aestivum L.) under irrigation withholding conditions .
Material and method:
A factorial experiment was conducted based on randomized complete block design with three replications at the research farm, faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili during 2018-2019. Factors experiment were included irrigation at three levels (full irrigation as control, irrigation withholding in 50% of heading and booting stages as moderate and severe water limitation respectively according with 43 and 55 BBCH scale) and foliar application of methanol at four levels (foliar application with water as control, application 10, 20 and 30 volume percent).
ResultsThe results showed that irrigation withholding decreased quantum yield, maximum fluorescence (Fm), variable fluorescence (Fv), chlorophyll index, relative water content, stomatal conductance and grain yield. Whereas electrical conductivity, electrolyte leakage and minimum fluorescence (F0) increased. Foliar application of 30 volume percent of methanol under full irrigation increased about 51.53, 170.75, 78.76, 55.69, 77.58, 79.4 and 46.98% maximum fluorescence (Fm), variable fluorescence (Fv), quantum yeild (Fv/Fm), chlorophyll index, relative water content, stomatal conductance and grain yield respectively in comparison with no foliar application of methanol under irrigation withholding in booting stage condition. Also, foliar application of 30 volume percent of methanol under full irrigation condition decreased electrical conductivity, electrolyte leakage and minimum fluorescence (F0 122.68, 73.07 and 42.59% respectively in comparison with no foliar application of methanol under irrigation withholding in booting stage condition
ConclusionBased on the results of this study, it seems foliar application of 30 volume percent of methanol can increase grain yield of wheat under water limitation condition due to improve fluorescence indices and some physiological traits such as stomatal conductance, relative water content and chlorophyll index
Keywords: chlorophyll content, Drought stress, relative water content, Stomatal conductance -
به منظور بررسی تاثیر یونیکونازول و کودهای زیستی بر فعالیت برخی آنزیم های آنتی اکسیدانی و اسمولیت های سازگار گندم رقم زاگرس در شرایط شوری خاک، آزمایش فاکتوریلی در قالب طرح پایه بلوک های کامل تصادفی در سه تکرار در گلخانه پژوهشی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال 1397 اجرا شد. فاکتورها شامل شوری خاک در چهار سطح (عدم اعمال شوری به عنوان شاهد و اعمال شوری های 40، 80 و 120 میلی مولار در خاک) با نمک کلرید سدیم و کاربرد یونیکونازول، قارچ میکوریز Glomus intraradicese و باکتری سودوموناس Psedumonas putida strain 186 در هفت سطح بود. مقایسه میانگین ها نشان داد کاربرد توام میکوریز با یونیکونازول و سودوموناس در شرایط شوری 120 میلی مولار، موجب افزایش محتوای پرولین، قند محلول و فعالیت آنزیم های آنتی اکسیدانت مانند پراکسیداز، کاتالاز و پلی فنل اکسیداز (به ترتیب 98/93، 33/47، 88/63، 67/75 و 35/69 درصد) نسبت به عدم کاربرد کودهای زیستی در عدم اعمال شوری شد. هم چنین کاربرد توام میکوریز با یونیکونازول و سودوموناس تحت شرایط عدم اعمال شوری، موجب افزایش محتوای پروتئین برگ، ارتفاع بوته و عملکرد دانه (به ترتیب 36/72، 85/41 و 84/108 درصدی) و کاهش نشت الکترولیت (02/39 درصدی) نسبت به شرایط کاربرد کودهای زیستی در بالاترین سطح از شوری خاک شد. به نظر می رسد کاربرد کودهای زیستی و یونیکونازول می تواند عملکرد دانه ی گندم را واسطه ی بهبود صفات بیوشیمیایی و فیزیولوژیک تحت شرایط شوری خاک افزایش دهد.کلید واژگان: پرولین, سودوموناس, قندهای محلول, کاتالاز, میکوریزIn order to study the effect of uniconazole and bio fertilizers on activity of some antioxidant enzymes and compatible osmolites of wheat (Triticum aestivum L var Zagros.) in soil salinity conditions, a factorial experiment was conducted based on randomized complete block design with three replications in a research greenhouse of the Faculty of Agriculture and Natural Resources of Mohaghegh Ardebili University in 2018. Treatments were included soil salinity in four levels (no application of salinity as control and application of 40, 80 and 120 mM soil salinity), by NaCl and application of uniconazole, mycorrhiza fungi (Glomus intraradicese) and pseudomonas putida strain 186 in seven levels. Means comparision showed that both application of mycorrhiza with uniconazole and pseudomonas under 120 mM salinity increased soluble sugars and proline contents, antioxidant enzymes activity such as catalase, peroxidase polyphenol oxidase (93.98, 47.33, 63.88, 75.67, 69.35% respectively) in comparison with no application of bio fertilizers under without salinity. Also, both application of mycorrhiza with uniconazole and pseudomonas under without salinity increased leaf proteine, plant height and grain yield (72.36, 41.85 and 108.84% respectively) and decreased electrolyte leakage (39.02%) in comparison with application of bio fertilizers under at the highest soil salinity level. It seems that the mentioned bio-fertilizers and uniconazole application can increase grain yield of wheat due to improve biochemical and physiological traits of wheat under soil salinity condition.Keywords: Catalase, Mycorrhiza, proline, Pseudomonas, Soluble sugars
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به منظور بررسی اثر متانول بر فعالیت آنزیم های آنتی اکسیدانی، برخی اسمولیت های سازگار و صفات بیوشیمیایی گندم تحت شرایط قطع آبیاری، این آزمایش به صورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی با سه تکرار در مزرعه پژوهشی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی در سال زراعی 98-1397 اجرا شد. فاکتورهای مورد بررسی شامل سطوح آبیاری (آبیاری کامل به عنوان شاهد، قطع آبیاری در 50 درصد مرحله سنبله دهی و قطع آبیاری در 50 درصد مرحله شکم خوش (آبستنی) به ترتیب به عنوان محدودیت ملایم و شدید آبی بر اساس کد 55 و 43 مقیاس BBCH) و محلول پاشی متانول در چهار سطح (محلول پاشی با آب به عنوان شاهد و محلول پاشی 10، 20 و 30 درصد حجمی متانول) بود. نتایج نشان داد محلول پاشی 30 درصد حجمی متانول در شرایط آبیاری کامل موجب افزایش 83/42، 01/36، 69/29، 93/57 و 98/49 درصدی محتوای کلروفیل b، کلروفیل کل، کارتنویید، درصد پروتئین و عملکرد دانه و کاهش 77/63 و 58/75 درصدی به ترتیب محتوای پراکسید هیدروژن و مالون دی آلدهید، نسبت به عدم محلول پاشی تحت شرایط قطع آبیاری در مرحله شکم خوش گردید. محلول پاشی 30 درصد حجمی متانول در شرایط قطع آبیاری در مرحله شکم خوش موجب افزایش محتوای پرولین و قندهای محلول (به ترتیب 42/125 و 93/91 درصد) نسبت به عدم محلول پاشی متانول در شرایط آبیاری کامل شد. همچنین محلول پاشی 30 درصد حجمی متانول در شرایط آبیاری کامل موجب کاهش فعالیت آنزیم های آنتی اکسیدانی (کاتالاز، پراکسیداز و پلی فنل اکسیداز) شد. به نظر می رسد محلول پاشی 30 درصد حجمی متانول می تواند عملکرد دانه گندم را در شرایط قطع آبیاری به واسطه ی بهبود صفات بیوشیمیایی افزایش دهد.
کلید واژگان: تنش خشکی, پرولین, مالون دی آلدهید و محتوای کلروفیلIn order to investigate the effect of methanol on the activity of antioxidant enzymes, some compatible osmolytes and biochemical traits of wheat under cut off irrigation conditions, the present experiment as factorial was conducted based on randomized complete blocks design with three replications at the research farm of Faculty of Agriculture and Natural Resources of University of Mohaghegh Ardabili, during 2015-2016 cropping year. The investigated factors were included irrigation levels (full irrigation as control, cut off irrigation in 50 percent of spike stage and cut off irrigation in 50 percent of the booting stage (pregnancy) as mild and sever water restriction, respectively; according to codes 55 and 43 of the BBCH scale) and methanol foliar application at four levels (foliar application with water as control and foliar application 10, 20 and 30 percent by volume of methanol). The results showed that foliar application of 30 percent by volume of methanol under full irrigation conditions increased 42.83, 36.01, 29.69, 57.93 and 49.98 percent of chlorophyll b content, total chlorophyll, carotenoids, protein percentage and grain yield and decreased hydrogen peroxide and malondialdehyde content 75.58 and 63.77 percent, respectively; in comparison with non-foliar application under cut off irrigation at the booting stage. Foliar application of 30 percent by volume of methanol under cut off irrigation conditions in booting stage increased proline and soluble sugars content (125.42 and 91.93 percent, respectively) in comparison with non-foliar application of methanol under full irrigation conditions. Also, foliar application of 30 percent by volume of methanol under full irrigation conditions decreased the activity of antioxidant enzymes (catalase, peroxidase and polyphenol oxidase). It seems that foliar application of 30 percent by volume of methanol can increase grain yield of wheat under cut off irrigation conditions due to improving biochemical traits.
Keywords: Drought tension, Proline, Malondialdehyde, Chlorophyll Content
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