fatemeh hakimian

Nanotechnology always seeks to provide new solutions for targeted delivery of chemotherapy drugs, in order to increase the quality of cancer treatment and reduce the side effects of chemotherapy. The aim of this study was to load the anticancer drug doxorubicin on Magnetic iron oxide nanoparticles were subjected to physico-chemical evaluation for their effect on MCF-7 cells.

This research was a descriptive-analytical study. In this laboratory research, iron oxide nanoparticles were first synthesized by precipitation method. Then they were coated with polyethylene imine and gold. After loading the drug doxorubicin into the magnetic iron oxide nanoparticles, the physiochemical parameters of the nanosystem from the point of view of insertion efficiency, drug release profile under similar conditions of healthy and cancer cells, size, zeta potential and morphology were determined.

The magnetic nanocarriers had a diameter of 90 nm and a zeta potential of 66.7 mV. The maximum release of the drug from the nanosystem at 37°C, pH=7.4 and 42°C, pH=5.4 and after 48 hourswas 48% and 66%, respectively. The SEM analysis showed the spherical morphology and the absence of chemical interaction between the nanosystem and the drug. The investigation of the performance of the nanosystem indicated an increase in the toxicity of encapsulated doxorubicin compared to free doxorubicin at similar concentrations on the MCF_7 strain.

The results of this research showed that the magnetic iron oxide nanoparticle system, while having appropriate physiochemical characteristics, does not change the chemical nature of the drug and can be a suitable and semi-targeted carrier for the anticancer drug doxorubicin.

Magnetic nanoparticles attract increasing interest due to their use in cancer therapy and as drug carriers for several other diseases. The present study investigates the physiochemical properties of quercetin-conjugated superparamagnetic Fe3O4 nanoparticles and their effects on breast cancer cell line MCF-7.

A simple precipitation method was used to prepare the Poly Ethylene Imine (PEI)-coated Fe3O4 nanoparticles; they were then conjugated with flavonoid-compound quercetin on the surface via carboxylic/amine group using nanoprecipitation method. Then, the physical and chemical parameters were calculated using Zeta-sizer, scanning electron microscopy (SEM), and extract release patterns at 37 and 42 0C. Finally, the toxicity level of this quercetin- conjugated nanosystem on the MCF-7 cells was investigated by MTT assay.

The results showed that the prepared nanosystem attained about 74% of quercetin inclusion, 91.2 nm size, 65.1 mV zeta potential, spherical morphology and a controlled release. Compared to Fe3O4 nanoparticles and pure quercetin, MTT and microscopy analysis revealed that quercetin-conjugated Fe3O4 nanoparticles induced considerable cytotoxicity, and morphology changes against MCF7 cells.

Quercetin-conjugated Fe3O4 nanoparticles have appropriate physiochemical properties; they can be a suitable carrier for drug delivery and a promising therapy for candidates.

Aim and Immobilized proteins and enzymes are commonly used in the medical field for diagnosis and treatment of various diseases. Due to the increasing use of immobilized enzymes and proteins in the diagnosis of disease, in this study, Cytochrome c protein was used as a useful metalloprotein in the basis studies of biosensors and Bioelectrochemistry.
Material and Cytochrome c protein immobilized covalently on the surface of gold and graphite pencil electrods. Surface of gold and graphite electrodes modified by gold nanoparticle and self-assemble monolayer of 3- mercaptopropionic acid. EDC and NHS as coupling agents cause to covalent attachment of protein on the surface.
Cyclic voltammetry results showed successful immobilization of Cytochrome c on the both surface. Immobilized protein on the surface of gold electrod was more stable than garaphite electrode.
In view of the Cytochrome c as a sample biological species, both surfaces can be suitable for immobilization of various biological species, and selection any of them depend on their application in various fields.

Antibacterial materials are so significant in the textile industry, water disinfection, medicine, and food packaging. Unfortunately, organic compounds for sterilization show toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing.
Nickel and nickel hydroxide nanoparticles (NiNPs and Ni(OH)2-NPs) were prepared and characterized by DLS, SEM, AFM and ATR. Antibacterial activity assay was carried by Spot on lawn method against two selected standard pathogenic bacteria such as E. coli (as Gram negative), S. aureus (as Gram positive) and multidrug resistance K. pneumonia and E. coli. Also the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against two selected standard pathogenic bacteria and multidrug resistance K. pneumonia and
E. coli.
The formation of the NiNPs and Ni(OH)2-NPs were confirmed by DLS, SEM, AFM and ATR. Antibacterial activity of nanoparticles were confirmed against two selected standard pathogenic bacteria such as E. coli and S. aureus. And also, NiNPs and Ni(OH)2-NPs revealed fair antibacterial effect against multidrug resistance K. pneumonia and E. coli based on MIC and MBC data. As well, the experimental data presented that the antibacterial activity of NiNPs was more than Ni(OH)2-NPs.
Based on the achieved results, NiNPs and Ni(OH)2-NPs show antibacterial activity against clinical patients bacteria (multidrug resistance K. pneumonia and E. coli(. Finally, the NPs evaluated in this study have promising properties for applications as antiseptic agent for environment; however, further studies are warranted such as study toxicity NPs on normal human cell line and other clinical bacteria.