gholamreza naderi

Hyssop is one of the medicinal plants that have many properties and benefits for the health of the body. Hyssop is an annual plant and often grows along roadsides. Hyssop leaves and green stems contain many bioactive compounds such as rosemary and Caffeic acid along with pinion, beta-pinna, limonene, pinoamophone and isopinocampone, tannins, glycosides, flavonoids, and essential oils. It is found that it can be produced purely by steam distillation from crushed leaves and stems. In this study, different bio fertilizers, chemical fertilizers, and manure were applied to find out their effects on the growth and active substances of Hyssop. Essential oils of Hyssop aerial parts were obtained by hydrodistillation and analysed by gas chromatography/mass spectrometry (GC/MS).  Essential oil percent was maximum (1.66 %) by application of N50P40K50 fertilizer and there was not any significant difference compared to control treatments. The highest value of oil yield (5.389 kg.ha-1) was achieved on cow manure treatment which increased oil yield compared to control (3.718 kg.ha-1). The highest value of Iso pinocamphone (43.32%) was obtained from plants that received N50P40K50 fertilizer compare to the control treatment (39.32%).  Control treatment had the highest value of Pinocamphone (14.11%) compared with cattle manure treatment (11.59%).

Acanthamoeba is a resistant protozoan that causes severe diseases, such as GAE and CAK. Because many medications are ineffective on the parasite, the quest to find alternative drugs is in progress.

This research was aim13 ed to assess the performance of the black and green tea extracts on Acanthamoeba.

The clinical isolate of Acanthamoeba was cultured on non-nutrient agar plates. The black and green tea extracts were prepared via maceration, dried by rotary evaporation, and stored at 4 °C. The main component of the extracts (caffeine) was recognized using GC-MS analysis. The effects of three concentrations of black and green tea extracts were evaluated on the parasite and compared with the control and current drug.

Black and green tea extracts have lethal effects on Acanthamoeba cysts and the performance was more than that shown by the control and the current anti-keratitis drug. Moreover, the effectiveness of the tea extracts was tim 25 e- and dose-dependent (P < 0.0001). There was no significant differences between the performance of black and green tea (P > 0.05).

Black 13 and green tea extracts demonstrated the potential to inhibit the Acanthamoeba parasite, but the use of tea extract in clinical applications requires further study.

This survey was conducted to study the relationship between total length of Capoeta gracilis (with selection of two length ranges, including TL>11cm and TLC. gracilis were different in Taleghan River. The results also revealed that the length class with total length of more than 11 cm selects habitats with higher elevation (p