فهرست مطالب hamed kolangi

The present study aimed to compare nonspecific immune parameters and protein pattern of  skin mucus in Neogobius pallasi and Neogobius melanostomus. The mucus samples were collected from 10 specimens ( 5 of each species ). Total protein, alkaline phosphatase ( ALP ), lysozyme and total immunoglobulins of the samples were measured (according to the porotocol). The measured levels of non-specific immune parameters in Neogobius pallasi were, 3.14±0.22, 41.19±0.89, 10.60±1.00 and 0.48±0.02 and in Neogobius melanostomus were 1.23±0.32, 30.88±1.52, 8.78±0.67 and 1.09±0.12 respectively. Non-specific immune parameters that measured in all instances, were higher in Neogobius pallasi and there were significant differences between the two species. Therefore, results showed that skin mucus has an important role in innate immunity and two species differences in response to stressful situations can be partly attributed to mucosal immune parameters.

Due to the increasing bacterial resistance to antibiotics and the side effects of chemical drugs, the tendency towards the use of medicinal plants has increased. The aim of present study was to investigate the effect of Ferula assafoetida hydroalkohalic extract on mucosal immune response (Alkalin phosphatase, total protein and total immunoglobin) in zebrafish. For this purpose 360 fish with an average weight of 0.3 ± 0.01 were randomly divided in 12 aquaria (three replicate) and fed diet supplemented with 0, 0.5, 1and 2 % ferula extract for 60 days. Evaluation of mucosal immune parameters revealed that supplementation of diet with ferula extract significantly elevated total immunoglobin activity in fish fed 1% compared to control and other treated groups (P<0.05) but Alkalin phosphatase and total protein didn’t show significant difference in fish fed supplemented diet compared to control (P>0.05).The mocus protein pattern showed ranges from 11 to 63 kDa. Diet including 1% showed the thickest band mocus protein pattern. Based on the results of this study, using 1% ferula hydroalkohalic extract is recommended for improvement of mucosal immune parameters in zebrafish.

In this study, effects of aloe vera on growth performance, Hematology and serum biochemical indices and the resistance to Saprolegnia parasitica were studied in rainbow trout for 8 weeks. Aloe vera powder at levels of 0, 5, 10 and 15 g/kg were added to a basal diet. At the end of trial period, the fish were exposed to the fungus S. parasitica. The results showed that all treatments fed with aloe vera-included diets showed significant increases in weight gain, specific growth rate and reduced feed efficiency compared to the control. Also fish treated with aloe vera showed significant increases in hematology and serum parameters in comparison with control. However, significant reduction in mortality was observed in the treated fish with 15 g/kg diet of aloe vera compared to the other treatments and the control following challenging with S. parasitica, but 5 g/kg diet of aloe vera survival rate increased significantly compared with control. According to the results, the use of aloe vera powder, especially at a level of 5 g/kg in rainbow trout diet is recommended in order to improve growth and immunity parameters as well as boosting fish resistance against S. parasitica contamination.

This study was conducted to find out the effect of bile salts in fish ration on indexes of growth, survival, carcass composition and some of the hematological and biological indexes of blood serum for this purpose 300 juvenile common carp (Cyprinus carpio) with an average weight of 3 ± 0.5 grams were studied for 12 weeks. Cow bile salts were added to the ration of fry fish at 5 different levels of 0, 0.25, 0.50, 0.75, and 1 percent during the experiment, two daily feedings were given at the ratio of 3 to 5% of the living mass. At the end of the experiment, data analysis revealed no significant differences in indexes of growth and survival, protein, ash, and carcass moisture between different treatment groups (P>0.05). Treatment group 4 with 1% bile salt had the least amount of fat, 32.52 ± 0.629%, and statistically, it was significantly different from other groups (P<0.05). Also, group 2 with 0.5% bile salts showed the highest and control group with 0% bile salts showed the lowest level of blood cholesterol and triglyceride, and therefore were significantly different from other groups (P< 0.05). Also the total amount of protein, white blood cells, red blood cells, hemoglobin, hematocrit and MCH, MCHC, MCV were significantly different between treatment groups and the control group (P< 0.05).