hamid taghavi
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مهم ترین عامل بازدارنده در استفاده از محصولات فرعی پسته در تغذیه دام، میزان بالای ترکیبات فنلی و تانن آن می باشد. این مطالعه جهت بررسی اثرات عصاره حاوی تانن محصولات فرعی پسته بر قابلیت هضم ظاهری مواد مغذی، توازن نیتروژن و فراسنجه های تخمیری شکمبه در نشخوارکنندگان انجام شد. تعداد 4 راس گوسفند نر بلوچی بالغ دارای فیستولای شکمبه ای در قفس های متابولیسمی انفرادی نگهداری شدند. عصاره آبی محصولات فرعی پسته پس از تغلیظ در 3 سطح برآورد شده جهت تامین 2، 4 و 6 درصد تانن کل در جیره به داخل شکمبه تزریق شد. نتایج نشان داد که تزریق عصاره تاثیر معنی داری بر مصرف خوراک، وزن بدن و قابلیت هضم ظاهری ماده خشک و پروتئین خام نداشت، اما سبب کاهش معنی دار قابلیت هضم ظاهری ماده آلی، NDF و ADF، و افزایش زمان مصرف خوراک شد. با افزایش غلظت عصاره، pH و نسبت استات به پروپیونات مایع شکمبه به طور معنی داری افزایش، و مقادیر نیتروژن آمونیاکی، پروپیونات، والرات و ایزو والرات مایع شکمبه به طور معنی داری کاهش یافت. با تزریق عصاره، دفع نیترژن از طریق ادرار کاهش، ولی از طریق مدفوع و همچنین نیتروژن ابقا شده افزایش یافت. به طور کلی نتایج آزمایش نشان داد که تزریق شکمبه ای عصاره جهت تامین 4 درصد جیره تانن کل ضمن حفظ قابلیت هضم ظاهری پروتئین خام، سبب بهبود توازن نیتروژن و ویژگی های تخمیر شکمبه ای گوسفندان شد.
کلید واژگان: تانن, تخمیر شکمبه ای, قابلیت هضم ظاهری, نشخوارکنندگانIntroductionAccording to the Food and Agriculture Organization (FAO), Iran is the largest pistachio (Pistachio vera) producer in the world. Nevertheless, pistachio byproducts (PBs) contain a high level of phenolic compounds and tannins, which can affect their utilization by animals. Tannins (hydrolysable and condensed tannin) are polyphenolic polymers of relatively high molecular weight with the capacity to form complexes mainly with proteins due to the presence of a large number of phenolic hydroxyl groups. They are considered as anti-nutritional compounds due to their adverse effects on intake and animal performance. However, tannins have been recognized to modulate rumen fermentation favourably such as reducing protein degradation in the rumen, prevention of bloat, inhibition of methanogenesis and increasing conjugated linoleic acid concentrations in ruminant-derived foods. The inclusion of tannins in diets has been shown to improve body weight and wool growth, milk yields and reproductive performance. Pistachio byproducts (PBs) contain a high level of phenolic compounds and tannins, which can affect their utilization by animals.The present study was conducted to evaluate the effects of PBs extract on nutrients apparent digestibility, nitrogen balance and ruminal fermentation characteristics in ruminants.
Materials and MethodsPBs were completely dried under sun and were ground to pass a 1 mm sieve by cyclic mill. PBs were soaked in water at 1 to 5 ratio in ambient temperature for 12 h and then were smoothed with the 4 layer cotton cloth. The extract was concentrated in an oven at a temperature below 40◦C. Four mature Baluchi male sheep (36.82 kg body weight, standard deviation 1) were fitted with ruminal cannula and were placed in individual metabolic cages with free access to fresh drinking water. The basal diet comprised 0.415 kg/d alfalfa, 0,165 kg/d barley straw and 0.250 kg/d concentrate feed (as-DM basis) that met their energy and crude protein (CP) requirements for maintenance according to the NRC (2007), and was offered in one meals. The concentrated PBs extract was infused in rumen at the levels that were administered to supply 0 (CON 1), 2, 4, or 6 % total tannin in the daily DM intake. All animals simultaneously received the same PBs extract dosage. Every period comprised 14 d of adaptation and 7 d of total urine and feces collection. Subsequent to highest PBs extract dosage, infusion was ceased and after 14 d of adaptation, urine and feces were collected again for 7 d (CON 2). Also in the end of every period rumen sampling was taken and animals were weighted.
Results and DiscussionFeed intake of animals were not affected by ruminal infusion of the different levels of PBs extract due to the restricted feeding level, but duration of feed intake was significantly increase when the concentration of PBs extract was increased. It seems, the intraruminal PBs extract application might not have interfered with diet palatability. The ruminal PBs extract infusion had no significant effect on body weight gain, DM and CP apparent digestibility but OM, NDF and ADF apparent digestibility were significantly reduced. Besides, the pronounced ability of tannins to form complexes with feed proteins; they may impair microbial degradation of other polymers such as cellulose, hemicellulose, pectin, and starch. The increasing of PBs extract concentration significantly increased rumen pH and acetate/propionate ratio and decreased rumen ammonia nitrogen, propionate, valerate and isovalerate. Lower ruminal ammonia nitrogen concentration may have resulted from a greater concentration of tannins that bound to proteins and decreased proteolysis resulting in a reduction of ammonia nitrogen release in rumen. Depression in VFA concentrations might be related to lower microbial activity of rumen in the presence of tannins. Iso-acids are derived from amino acids catabolism by cellulolytic bacteria in the rumen, that reductions their concentrations can indicate the protection of protein from bacterial deamination. Higher ruminal pH may have resulted from lower ammonia nitrogen and VFA concentrations in the rumen. The urine nitrogen excretion was reduced by increasing of PBs extract concentration, while the feces nitrogen excretion and retained nitrogen were increased. Infusion of incremental PBs extract dosages greatly shifted partitioning in nitrogen from urine to feces and could contribute to lower ammonia emissions from manure in ruminant production. The decrease in urinary nitrogen excretion can be attributed to the protein-binding property of PBs extract and the consequently lower ruminal protein degradation and ammonia nitrogen release. Besides of the tannin level, the effects of tannins differ greatly by the type and source of tannin as well as the composition of the animals’ diet.
ConclusionGenerally results of this experiment indicated that while the ruminal PBs extract infusion at the level that was administered to supply 4 % total tannin in the daily DM intake preserved CP apparent digestibility, it improved nitrogen balance and rumen fermentation characteristic of sheep.
Keywords: Apparent digestibility, Tannin, Rumen fermentation, Ruminants -
Background
Gold nanoparticles with high atomic number and density have good potential to be used as contrast media in computed tomography.
ObjectivesIn this study, we aimed to assess radiation dose and contrast enhancement performance of gold nanoparticles by measuring contrast to noise ratio compared to clinically used iodinated contrast agents at same concentrations. Contrast enhancement was evaluated in different tube voltages and currents.
MethodsFirst, polyethylene glycol coated gold nanoparticles were synthesized with concentrations of 0.5 mM, 0.6 mM, and 0.7 mM. Gold nanoparticles and iodinated contrast media were scanned with CT imaging system at different tube voltages and time-current product. CT dose index (CTDI) value was measured by special phantom and electrometer. Improving in image contrast was assessed by contrast to noise ratio.
ResultsResults showed that gold nanoparticles in all concentrations and energies from 80 to 130 kVp display higher image contrast-to-noise ratio (CNR) than iodinated contrast media. Image CNR was increased by increasing kVp and mAs. The CNR value was maximum at the voltage of 80 and 130 kVp for iodinated compounds and gold nanoparticles, respectively. The CNR value for gold nanoparticles at 130 kVp and 200 mAs was approximately five times higher than that of iodinated compounds.
ConclusionGold nanoparticles could be a good candidate for optimizing CT imaging by lowering radiation dose as low as possible while enhancing the image contrast.
Keywords: Computed Tomography, Gold Nanoparticles, Radiation Dose, Iodinated Compounds, Image Contrast -
این مطالعه به منظور بررسی امکان پیش بینی وقوع تنش گرمایی در گاوهای شیری و تعیین دوره های بحرانی آب و هوایی انجام شد.رایج ترین شاخص جهت برآورد میزان تنش گرمایی در گاوهای شیری، شاخص رطوبتی-دمایی(THI) است. در این مطالعه،شاخصTHIیوسف (1985) برای تجزیه و تحلیل اثرات دما و رطوبت مورد استفاده قرار گرفت. داده های هواشناسی از ایستگاه تحقیقات هواشناسی شمال شرق ایران گرفته شد. پس از محاسبهTHI، تغییرات آن به کمک نمودارهای پراکندگی بررسی ومقادیرTHIتوسط طرح فاکتوریل دو عاملی آنالیزشد. ازنظر آماری،تغییرات THI روند ثابتی در طول سال داشتند. اثرات سال معنی دار بود ومیانگین های ماهانهTHI تفاوت های معنی داری در طول سال داشتند.بالاترین مقادیرTHI در ماه جولای (11 تیر تا 10 مرداد)بدست آمدکه مقادیر THIدر بسیاری ساعات بالاتر از مقادیر آستانه احتمال وقوع تنش حرارتی بود. همچنین تعداد 186291 رکورد تولید شیر یک گاوداری بزرگ صنعتی مورد تجزیه و تحلیل قرار گرفت. اطلاعات بدست آمده نشان داد که تولید شیر نیز روندی ثابت، اما مخالف تغییراتTHI در طول سال داشت؛ به نحوی که با افزایش مقدار THI و عبور آن از مقادیر آستانه احتمال وقوع تنش گرمایی، میزان تولید شیر کاهش یافته و در ماه جولای به کمترین مقدار خود می رسید. به طور کلی شاخص THI جهت تعیین دوره های بحرانی آب و هوایی و پیش بینی احتمال وقوع تنش گرمایی در گاوهای شیری موثر بوده و می تواند راهنمای مناسب گاوداران جهت انجام به موقع اقدامات پیش گیرانه باشد.
کلید واژگان: شاخص رطوبتی دمایی (THI), گاو شیری, استرس گرمایی, تولید شیرThis study was carried out to investigate the possibility of prediction the occurrence of heat stress in dairy cows and determination of climate critical periods. The most common indexes for estimation the degree of thermal stress istemperature humidity index (THI). In this study Yousef’s THI index (1985)was used to analyze the yearly and monthly effects of temperature and humidity. Meteorological data were obtained from the Northeast Regional Climate Center of Iran.After theTHI was calculated, the variability of THI was analyzed using scatter charts and THI values were analyzed as two-factor factorial design. Statistically, the changes of THI had constant trend over the year. Effects of years were meaningful and THI monthly means had significant differences during the year. The highest values of THI were evaluated in July that THI values in many hours were higher than the threshold values of the occurrence of heat stress. Moreover, 186291 records of milk production from a large industrial dairy farm were analyzed. Data showed that milk production also had constant trend over the year but with opposite direction of THI. In other words, when the amount of THI increased and passed of the threshold values of the occurrence of heat stress, milk production reduced and reached to the lowest level in July.Generally THI index is effective for determination of the critical periods of climate and prediction the probability of heat stress in dairy cows, and it can be a perfect guidefordairy farmersto perform adequate preventive acts.Keywords: Temperature Humidity Index (THI), Dairy cattle, Heat stress, Milk production
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