hao wu

Colon adenocarcinoma (COAD) is one of the most common cancer happened in gastrointestinal tract, with the overall incidence rate of 4%-5% among human beings. Like most malignancies, we uncovered the exact mechanisms of the pathogenesis of colorectal cancer yet. Therefore, there is an urgent need to explore the molecules that can be used as diagnostic maker at early stage. In addition, we also need to define the essential factors that related to the prognosis and treatment of the colon carcinoma.

The study was conducted at the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, China in September 2020. The R language was used to identify the differentially expressed genes. We performed receiver operating characteristic curve analysis to determine the diagnostic markers for COAD. The machine learning strategy was used to assess the effectiveness of genes in the diagnosis of COAD. The molecular mechanism and prognostic value of genes were explored by bioinformatics analysis and molecular experiments.

The expression level of heat shock factor 4 (HSF4) was significantly elevated in COAD patients (P=1.89×10-29), according to The Cancer Genome Atlas (TCGA) database. Additionally, survival analysis showed the higher expression level of the HSF4 was correlated with the poor prognosis in COAD.

The HSF4 was the target gene of zinc finger protein 692(ZNF692). HSF4 might promote the progression of COAD through the apoptosis pathway. It was diagnostic and prognosis maker of COAD. Furthermore, the upstream gene of HSF4, ZNF692, promotes the progression of colorectal cancer by regulating HSF4 expression.

This study aimed to observe the clinical effect of modified Devine’s surgical technique in the treatment of concealed penis.

From July 2015 to September 2020, fifty-six children with concealed penis were treated with modified Devine’s technique. Recorded the penile length and the satisfaction score preoperatively and postoperatively to confirm the effect of the surgery. Followed up the penis for bleeding, infection and edema one week and four weeks after the operation. Twelve weeks after the operation, we measured the length of the penis and observed whether there was a retraction.

The length of the penis has been effectively lengthened(P < 0.001). There was significant improvement in parents’ satisfaction grades (P < 0.001). All the patients had different degrees of penile edema after the operation. Most of the penile edema subsided about four weeks after the operation. No other complications occurred. No obvious penile retraction was found twelve weeks postoperative.

The modified Devine’s technique was safe and effective. As a treatment for concealed penis, it is worthy of wide clinical application.

Rapid maxillary expansion (RME) is the standard treatment for correcting lateral maxillary defects commonly used in orthodontics. It is the most effective approach to increase maxillary width in clinical practice. At present, there are few studies on the level of molecular biology of periodontal tissue remodeling during RME. We aimed to investigate changes in matrix metalloproteinase (MMP)-8, interleukin (IL)-6 and tumor necrosis factor (TNF)-αin gingival crevicular fluid during RME.

Patients admitted to Department of Stomatology, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China between Dec 2016 and Dec 2018 were enrolled, and randomly divided into the observation group (76 cases) and control group (62 cases). Periodontal clinical indicators were recorded. Gingival crevicular fluid was collected and the periodontal clinical indicators were recorded. The levels of MMP-8, IL-6 and TNF-α were determined by ELISA, and the contents of the two groups were compared.

The plaque index of the observation group was significantly higher than that of the baseline T0 (P< 0.05) from T4, and the increase in the control group started from T5. The general clinical data of the two groups showed that the white blood cell count of the observation group was higher than that of the control group. The levels of MMP-8, IL-6 and TNF-α were the highest at T2, followed by T3, and gradually decreased at T4, and T5, and the differences were significant (P<0.05).

The changes in levels of MMP-8, IL-6 and TNF-α in adolescent patients during RME were related to the remodeling of periodontal tissue after RME.

The present study mainly aims at the identification and laboratory production of geopolymer concrete with the increased resistance to acid attack. For this purpose, after evaluating the reactivity of the raw materials and their applicability in the production of geopolymer cement among the available aluminosilicate materials, the metakaolin pozzolan was selected as the appropriate raw material. After the selection of the binder and activator of sodium hydroxide, the alkali activation process was performed for the mixtures of raw materials. Then, for the initial estimate of the durability performance of geopolymer cement  in acidic media, the durability of concrete samples was investigated in 1M sulfuric acid for 7, 28 and 90 days. The results showed that the nano-silica and nano-clay are effective in improving the performance of geopolymer concrete, as the addition of 3% nano-silica resulted in the 0.44% increase in the strength of the geopolymer concrete. Also, the addition of 3% nano-clay led to the increase in the strength of geopolymer samples up to 0.54. In addition, the samples are more durable in acidic media, so the weight loss of nano-clay-containing geopolymer samples in 90 days is 1.2 times that of geopolymer concrete samples without this additive.

Carbon monoxide (CO) poisoning is one of the most important intoxications in the modern world. This study aimed to identify the characteristics of CO poisoning deaths in Sichuan province in the west of China. Data on fatal non-fire-related carbon monoxide poisoning in Sichuan from 2008 to 2016 were obtained from the Department of Forensic Analytical Toxicology of Sichuan University and were analyzed by the month and year of registration of death, sex, age group, manner of death, source of CO, and location of CO exposure. Comparing with the previous studies carried out in Wuhan and Shanghai to identify the regional differences of CO poisoning in China. A total of 165 non-fire related CO poisoning cases including 237 victims were recorded. Over 90% of the victims died from accidental poisoning. Non-fire related CO poisoning occurred more frequently in winter months and was most prevalent in individuals aged between 18 and 60 yr old. Showering gas accident and coal or charcoal burning was found to be the major source of CO in accident and in suicide cases, respectively. Furthermore, significant regional differences of CO poisoning have been detected in the manner of death and the source of CO. These findings will be valuable in the targeted prevention of non-fire related CO poisoning in China.

Vancomycin is widely used for infections caused by gram-positive bacteria, but little attention has been paid to vancomycin in the treatment of critically ill patients aged ≥80 years. The aim of the current study was to investigate the efficacy of vancomycin and risk factors associated with nephrotoxicity of vancomycin in elderly critically ill patients.
A retrospective study was performed in a 14-bed medical-surgical geriatric ICU between January 2007 and June 2014. The patients (aged ≥80 years) were included if they received ≥4 doses of vancomycin and the therapy duration was ≥ 2 hours.
The clinical efficacy was 74.0% (37/50). The 28-day mortality was 26.0% (13/50). Of the patients, 24% (12/50) had nephrotoxicity during vancomycin treatment period. The clinical efficacy was 60%, 86.7%, 58.3%, and 33.3%, and the 28-day mortality rate was 20%, 23.3%, 33.3%, and 33.3%, respectively, when the trough concentrations were ≤10 μg/mL, 10–15 μg/mL, 15–20 μg/mL, and ≥20 μg/mL. The multivariate logistic regression analysis showed that an Acute Physiology and Chronic Health Evaluation (APACHE) II score ≥25 points, vancomycin trough concentrations ≥15 μg/mL, and the combined use of diuretics (furosemide ≥40 mg/d) were independent risk factors leading to nephrotoxicity.
We did not find that higher vancomycin trough concentrations lead to better clinical outcomes in elderly critically ill patients. Increased vancomycin trough concentrations, high APACHE II scores, and the combined use of diuretics may increase the risks of nephrotoxicity in elderly critically ill patients.

Up-regulation of CD4+CD25+CD127low/- regulatory T cells (Tregs) is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells can up-regulate CD4+CD25+CD127low/- regulatory T cells in vitro, meanwhile, heme oxygenase-1 (HO-1) plays an important role in the development and maintenance of CD4+CD25+ regulatory T cells. However the mechanism has not yet been adequately understood. Hence, we wondered what effect of Heme Oxygenase-1 made on regulation of CD4+CD25+CD127low/- regulatory T cells mediated by mesenchymal stem cells.Peripheral blood mononuclear cells isolated from asthmatic patients and healthy controls were co-cultured with human bone marrow mesenchymal stem cells which were pretreated with Hemin (the revulsive of Heme Oxygenase-1), Protoporphyrin Ⅸ zinc (the inhibitor of Heme Oxygenase-1) and saline.The expression of Heme Oxygenase-1 in MSCs was enhanced by Hemin and inhibited by Protoporphyrin zinc in vitro. Overexpression of Heme Oxygenase-1 elevated the proportion of CD4+CD25+CD127low/- regulatory T cells in CD4+ T cells, meanwhile, inhibition of Heme Oxygenase-1 decreased the proportion of CD4+CD25+CD127low/- regulatory T cells in CD4+ T cells as compared with mesenchymal stem cells alone.Taken together, these data demonstrated that Heme Oxygenase-1 contributed to the up-regulation of CD4+CD25+CD127low/- regulatory T cells mediated by mesenchymal stem cells in asthma.