heidar tavilani tavilani

subsets of human spermatozoa isolated by density gradient.Rohollah Setarehbadi 1; Mojgan Atabakhash 1; Amir Fattahi 1; Aboozar Mohagheghi 1; Asad Vaisi-Raygani 2; Hossain Mahjub 3; and Heidar Tavilani Tavilani *, 4 1 Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran2 Fertility and Infertility Center, Kermanshah University of Medical Sciences, Kermanshah, Iran3 Department of Epidemiology and Biostatistics, Faculty of Public Health, Hamadan University of Medical Science, Hamadan, Iran4 Urology & Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan Iran*Corresponding author: Heidar Tavilani Tavilani, Urology & Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan Iran, Tel.: +98 8118380717, Fax: +98 8118380313, E-mail: tavilani@gmail.com. Lipid components of spermatozoa have an important role in its functional activity. In this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous PureSperm gradient, was determined. Aliquots of the liquefied semen samples (n=107) were layered on top of the upper layer of 40 and 80% PureSperm gradient. The resulting interfaces 40 and 80% (fraction 1), 80% and pellet (fraction 2), and pellet (fraction 3) aspirated and transferred into separate tubes. Lipids were extracted with 6 volumes of chloroform-methanol (2/1, V/V) and the concentration of cholesterol, phospholipid, triacylglycerol were determined by colorimetric method. Percent of sperm with normal morphology was significantly higher in sperm fraction 3 compared to fraction 2 and 1, while percent of sperm with midpiece and tail defect was significantly higher in fraction 1 compared to fractions 2 and 3 (P<0.01). The amount of cholesterol, phospholipid and total lipid of sperm fractions 2 and 3 were about 1.5-fold higher than that of found in sperm from fraction 1 (P<0.05). This result suggests that the total lipid contents of sperms increase with increasing normal morphology from fraction 1 to 3.

Anti-oxidant effects of propofol (2, 6-diisopropylphenol) were evaluated agains carbon tetrachloridet CCl4 -induced oxidative stress in rat liver. 30 male rats were equally divided in to 6 groups (5 rats each). Group I (control), while Group II was given CCl4 (3 mL /Kg/day, IP). Animals of Groups III received only propofol (10 mg/Kg/day, IP). Group IV was given propofol+ CCl4. Group V was administered vitamin E (alpha-tocopherol acetate 15 mg/Kg/day, SC). Animals of Group VII received alpha-tocopherol acetate + CCl4 once daily for two weeks. After treatment, blood and liver mitochondria were isolated. Anti-oxidant enzymes activity such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and oxidative stress marker such as reduced glutathione (GSH) and lipid peroxidation (LPO) concentration were measured. Oxidative stress induced with CCl4 in liver mitochondria was evident by a significant increase in enzymatic activities of GPx, SOD, and LPO and decreased of GSH and vailability of mitochondria. Propofol and vitamin E restored CCl4-induced changes in GSH, GPx, SOD and LPO in blood and liver mitochondria. CCl4 decreased viability of mitochondria that was recovered by propofol and vitamin E. It is concluded that oxidative damage is the mechanism of toxicity of CCl4 in the mitochondria that can be recovered by propofol comparable to vitamin E.