فهرست مطالب homayun dolatkhah

 In recent years, the emergence of multidrug resistance in gastric cancer has been a major challenge in treatment of gastric cancer. To deal with the problem, studies and researches were conducted on Sclareol and have turned up the anti-cancer effect of the compound and have also determined the molecular mechanism of it to some extent. Therefore, the main purpose of this study was to investigate the effect of the substance extracted from Salvia Officinalis called Sclareol on MDR-1 gene expression and consequently on the rate of P-glycoprotein in human gastric cancer cell line MKN-45.

Cell line MKN-45 was purchased from the Pasteur Institute of Iran and cultured in complete RPMI 1640 Medium with Fetal Bovine Serum, with 20, 40, 60, 80 and 100 μM concentrations of Sclareol treatment for 5 hours. The rate of expression of MDR-1 gene was assessed by Real Time-PCR method and that of P-GP was assessed by Western blotting method.

The expression of MDR-1 gene was significantly reduced at doses of 20, 40 and 60 μmol of Sclareol, while at doses of 80 and 100 μmol there was not seen much effect (p <0.0001). Also, P-glycoprotein showed a very high decrease at doses of 40 and 60 μmol of Sclareol, but no decrease was seen at doses of 80 and 100 μmol (p <0.0001).

From the results of this study, it seems that doses between 20 and 60 μmol of Sclareol can be useful in reducing drug resistance, but doses above 60 mmol do not have such an effect.

Helicobacter pylori is the cause of many stomach diseases such as chronic gastritis, peptic lesion, gastric cancer, and dyspepsia. It is indicated that the process of inflammation is associated with nitrosative stress. Besides, polyunsaturated fatty acids (PUFAs) have strong protective effects against inflammatory diseases, as well as bactericidal effects. Therefore, this study evaluated the beneficiary effects of PUFAs against nitrosative stress in patients infected with this bacterium with dyspeptic symptoms.

This study was a double-blinded clinical trial and the participants were 34 patients infected with H. pylori with dyspeptic symptoms. The patients were divided into 2 groups, and written consent was obtained from all participants. The control group was treated with a current antibiotic regimen and the case group was treated with an antibiotics regimen and PUFA supplement for 2 weeks. Then, biopsy and juice samples from patients’ stomachs were obtained before and after the treatment. The stomach biopsies were used for a quick urease test and juice samples were used via the Griess method for investigating nitric oxide (NO) levels.

In gastric mucosa, the mean levels of NO significantly reduced (P<0.0001) after treatment in the case group (5.36 ± 1.16 mmol/L( compared with the control group (2.72 ± 1.13 mmol/L).

The results showed that using omega supplement can improve the nitrosative stress occurred in patients with H. pylori infections and is useful for decreasing NO. Thus, it is suggested that the consumption of omega fatty acids in combination with standard therapy is effective in H. pylori eradication.

Behcet’s disease (BD) is a multi-systemic inflammatory disorder. Evaluating theproduction of cytokines such as interferon gamma,biomarkers such as heat shock protein-70(HSP70)is an important way to study the pathogenesis,development of BD. This study aimed tocompare the salivary level of interferon gamma,HSP70 between patients infected with BD andhealthy individual.

This case-control study was performed on 35 patients with Behcet’s syndrome,70 healthyindividuals as the control group,who were selected from those referring to the Department of OralMedicine of Tabriz University of Medical Sciences. The levels of interferon gamma,HSP70 weremeasured in the whole unstimulated saliva through enzyme-linked immunosorbent assay (ELIZA). Inorder to compare the quantitative variables between two groups,independent samples t-test or itsnonparametric equivalent,Mann-Whitney U test,was used in SPSS software version 16.0. In this study,a P value less than 0.05 was considered statistically significant.

There was no significant difference between the study groups in terms of age,gender,aswell as salivary interferon gamma,HSP70 levels. Interferon gamma level was 15.16±3.38 pg,mg inthe case group,5.27±1.21 pg,mg in the control group,and salivary HSP70 level was found to be45.50±17 ng,mL,19.5±5.2 ng,mL in the case,control groups,respectively.

The results of this study showed that interferon gamma,HSP70 levels in patients withBehcet’s syndrome are high,can be evaluated as an important tool for the treatment,evaluationof disease development in future studies

Drug-resistant strains and poor treatment response is the main reason for the failure in Helicobacter pylori (H. pylori) eradication. Unsaturated fatty acids with more than one double bond (PUFAs) have inhibitory effect on bacterial growth. Therefore, the aim of this study is to investigate the effect of Omega fatty acids on caspase-8 gene expression in H. pylori infected patients with dyspeptic symptoms.

In this double blind clinical trial study in which 34 H.pylori infected patients with dyspeptic symptoms were randomly divided into two groups of 17 patients, the control group receiving standard triple therapy (including amoxicillin, clarithromycin and omeprazole) and the intervention group receiving standard therapy with omega-3, omega-6 and omega-9 fatty acids for two weeks. After treatment, Gene expression levels of Caspase-8 enzymes as well as the semi-quantitative of these proteins were studied with PCR Real-time method and frozen section and immunohistochemistry, respectively.

In the case group compared with the control group, a significant increasing in the expression of caspase -8 genes was observed (p=0.002). The protein level of active caspase -8 enzymes was significantly increased in the case group compared with the control group (0.004).

The Omega fatty acids along with standard triple therapy lead induce apoptosis is stomach.

 The use of some active factors in diet is regarded as an attractive approach to prevent and to treat certain types of cancers. Accordingly, the main objective of this study was to evaluate the effect of PUFAs oral administration along with chemotherapeutic agent on the level of cellular apoptotic regulatory proteins in cancer cells of individuals with gastric cancer in order to identify the apoptotic changes.

This study was a Clinical Trial in which the target group consisted of the patients with gastric cancer who were recognized for the first time and cured under chemotherapy. Thirty-four patients were chosen and categorized randomly into two groups. Case group includes the patients taking PUFAs along with the chemotherapeutic agents. In control group, individuals were under the same chemotherapy protocol without taking PUFAs. Biopsy samples of tumor were taken from the patients before and after chemotherapy. The Bcl-2, Bcl-XL Bid, and Bad gene expression were determined by Real-Time PCR. Also, those proteins upon biopsy samples were surveyed by Frozen Section method.

In case group, Bcl-2 and BclXL gene expression and protein levels decreased significantly in comparison with those of the control group. While Bid and Bad gene expression and protein levels increased significantly in comparison with those of the control group.

The results of this study showed that use of PUFAs as supplement with Cis-platinum may be useful to stimulate more pro-apoptotic proteins in gastric cancer cells. Consequently, this offers an effective treatment to patients with gastric cancer to respond to chemotherapy.

Gastric cancer is the 5th most common cancer and the third cause of death in the world. Studies have shown that gastric cancer is somewhat susceptible to chemotherapy, but the duration of tumour reduction is short, and patients have not had much success in survival, and in many cases, chemotherapy resistance has been observed. Therefore, the main objective of this study was to investigate the effect of omega-unsaturated fatty acids on the expression of Cyclin A2, and CDK2 germ cell cycle in patients with gastric adenocarcinoma under chemotherapy.

This is a double-blind, pre-and post-test clinical trial with the target population of patients with gastric adenocarcinoma that were first identified and subjected to chemotherapy. Twenty-four patients were selected randomly and randomly in control and control groups. In the control group, the treatment was routine with cisplatin plus placebo. In the case group, treatment with cisplatin plus a supplement of natural fatty acid supplementation capsules of Ultimate-Omega Factors with a dose of 1200 mg per day was 3,600 mg Three tablets of 1200 mg (for three courses) started on horizons three weeks. Three samples of stomach biopsy were taken from all patients before and after chemotherapy. Biopsy specimens were extracted from all tissue mRNAs and cDNA was synthesized from them, and then the expression of the genes was measured using Real-Time PCR. The results were analyzed by SPSS software version 24.

The mean or average expression of Cyclin A2, CDK2 in the case group showed a significant decrease compared to the control group (P value was 0.021 and 0.026, respectively.

The results of this study showed that the use of omega-3, 6, 9 fatty acids with cisplatin can be useful in stopping the S-cell cycle in gastric cancer cells.

Gastric cancer is the fourth most common cancer and the second cause of death in the world. According to the study, gastric cancer cells have a relative resistance to chemotherapy. Therefore, the purpose of this study was to evaluate the effect of oral administration of omega fatty acids PUFAs) on lipid peroxidation and oxidative stress in gastric cancer in people with gastric cancer before and after chemotherapy.

This is a double-blind clinical trial. The target group was gastric cancer patients who were first identified and under chemotherapy. Among them, 30 were selected and randomly assigned to two groups. they got. In the control group, the treatment was routine with cisplatin plus placebo. In the case group, treatment with cisplatin plus supplemental capsules of Natural Factors Ultimate-Omega Factors with a dose of 1200 mg per day was 3,600 mg Three tablets of 1200 mg (for three courses) started on horizons three weeks. Three samples of stomach biopsy were taken from all patients before and after chemotherapy. Lipid peroxidation was measured by the Thiobarbituric Acid method and the activity of superoxide dismutase and glutathione peroxidase enzymes by Randox kit. To compare the results, Independent- t-test and Mann-Whitney SPSS software.

In the case group, the percentage of lipid peroxidation of the gastric cancer tissue after omega-unsaturated fatty acids increased significantly (P value less than 0.0001). Omega fatty acids caused a significant reduction in the activity of the enzyme superoxide dismutase in the case group was statistically lower than the control group (P value less than 0.0001)

The results of this study indicate that the use of omega fatty acids as a complement to cisplatin for controlling gastric cancer can be helpful in increasing the amount of oxidative stress and lipid peroxidation of the gastric cancer tissue in this The study was shown.