فهرست مطالب hosein hooshyar

Toxoplasma gondii is an opportunistic parasitic protozoon, which is a causative agent of serious complications such as encephalitis in immunodeficiency patients. Considering insufficient data on toxoplasmosis in these patients, the present study was conducted to determine the seroepidemiology of T. gondii among immunodeficiency patients.
This cross-sectional study was conducted on cancer, ADIS, hemodialysis and renal transplant patients (case group) and healthy persons (control group) in Kashan and Qom cities. Totally, 317 serum samples were collected from the case group and 120 samples from the control group. The ELISA method was used to determine the anti-T. gondii IgG and IgM antibodies.
Totally, 60.3% of the samples from the case and 33.3% from the control groups were positive for anti-T. gondii IgG (P The prevalence of T. gondii was higher in different immunodeficiency patients compared to healthy persons. Screening examinations were recommended for the diagnosis and treatment of patients to prevent serious side-effects and health education.

Hydatidosis is one of the most important zoonotic parasitic disease worldwide caused by Echinococcus granulosus. Given the importance of parasite genotyping in disease control, this study was aimed to evaluate the genetic variation of the camel and dog isolates in Isfahan.
Material and This cross-sectional study was done on infected livers and lungs of camels with hydatid cyst (n=51) during 2015-2016, collected from Zarinshahr and Najafabad industrial abattoir and 200 adult E. granulosus worms collected from stray dogs in different geographical regions of Isfahan for the molecular identification of mitochondrial genes (CO1, ND1). The DNA of protoscolices and adult worms were extracted, the PCR and sequencing were done using specific primers. Pairwise sequence alignment was used to identify the genetic similarity between the sequences and the registered ones in Gen bank using Clustalw2 software.
The PCR and sequencing of CO1 and ND1 genes on dog and camel samples showed different E. granulosus genotypes, including the common sheep strain (G1), bovid strain(G3) and camel strain (G6) in Isfahan region. The most frequent strains were the common sheep strain (G1). The genetic similarity between both ND1 and CO1 isolates taken from camel, dog and the isolates registered in Gene bank were 30.25-100% and 27.47-100% for camel and dog, respectively.
Mitochondrial ND1 and CO1 genes are proper molecular markers for identifying the genetic variation of the camel and dog E. granulosus. The dominant strain of the worm in Isfahan, is the same as the other parts of the country, i.e., the G1 genotype (the most common sheep strain) belonged to the G1- G1 E. granulosus complex.

Trichomoniasis is the most common sexually transmitted parasite in the world. The mainstay treatment for trichomoniasis is metronidazole, but some resistant strains to this treatment have also been detected. Considering the therapeutic properties of Pelarqonium roseum in traditional medicine, this study aimed to examine the antitrichomonal effects of Pelarqonium in vitro. In this experimental study, 1203 persons referred to Kashan Medical Centers randomly tested for Trichomonas vaginalis. Of them, 23 persons were infected with T. vaginalis. Three T. vaginalis isolates were cultured in a TYI-S-33 medium and were used to study the effect of Pelarqonium extract. Different concentrations (12.5, 25, 50,100,200,400,800, 1000µg/ml) of Pelarqonium extracts were tested in three different times (24, 48 and 72h) on T. vaginalis trophozoeites. Also the effect of metronidazole (0.025, 0.05, 0.1, 0.2, 0.4 µg/ml) was investigated as the drug control. In all tests the numbers of live and dead parasites were counted by trypan blue staining. Result showed that the alcoholic and aqueous extracts of Pelarqonium had the inhibitory effects on the growth of T. vaginalis trophozoeites. The IC50 of the aqueous and alcoholic extracts of Pelarqonium and metronidazole after 24h were 54.67, 27.63 and 0.0326, respectively. While the alcoholic and aqueous extract of Pelarqonium have anti-Trichomonas effect, the anti-Trichomonas properties of alcoholic extract is more than its aqueous extract. Since Pelarqonium is a herbal drug, it can be tested in vivo.