hossein arouei

In this study, the effects of different levels of biofertilizer containing phosphate solubilizing bacteria on aerobic digestion of Nile tilapia (Oreochromis niloticus) sludge were investigated in vitro under a completely randomized design. Three experimental treatments including aerobic digestion of Nile tilapia solid sludge without adding biofertilizer (control) and aerobic digestion of solid sludge with adding biofertilizer containing phosphate solubilizing bacteria (Pseudomonas putida strain P13 and Pantoea agglomerans strain P5) at levels of 0.1% and 0.2% were used in triplicate. The aerobic digestion containers with a capacity of 1 L (9 containers) were kept in a shaker incubator at 30ºC for 28 days. The results showed that the concentration of phosphate, potassium, and iron nutrients on the 14th day of the experiment in the biofertilizer treatments were significantly higher than the control, but their concentrations on the 28th day in the biofertilizer-0.1% treatment were significantly higher than other treatments )p<0/05). The manganese concentration on the 14th and 28th days in the biofertilized-0.1% treatment was higher than other treatments (p<0/05). The results of the present study indicated that the aerobic digestion of fish sludge for two weeks with adding biofertilizer containing phosphate solubilizing bacteria (P. putida strain P13 and P .agglomerans strain P5) by counting 107 of each bacteria per mL (CFU/mL) at 0.1% improved the release and dissolution of nutrients.

Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.