فهرست مطالب hossein saghi

Type 2 diabetes mellitus (T2DM) is a condition characterized by insufficient insulin production or insulin resistance. The insulin-degrading enzyme (IDE) is responsible for degrading insulin and is a potential drug target for T2DM treatment. Numerous activities have been proposed for plant extracts, but research on the effects of plant extracts on IDE expression and activity is riddled with drawbacks.

We investigated the effect of Phaseolus vulgaris, Allium cepa, Portulaca oleracea, Cinnamomum verum, and Citrullus colocynthis extracts on the expression and activity of IDE in the Caco-2 cell line.

Findings of RT-PCR showed that IDE gene expression was reduced following treatment with P. vulgaris, C. colocynthis, and C. verum extracts. The results of IDE activity with fluorogenic peptide substrate V also indicated that P. vulgaris, C. colocynthis, and P. oleracea extracts reduced IDE activity in a significant and dose-dependent manner.

The hydroalcoholic extracts studied, except for A. cepa, can prevent insulin degradation by reducing the expression and activity of the IDE enzyme. This new insight into the effects of herbal medicines on IDE activity can help future studies.

Acinetobacter baumannii is one of the Gram-negative bacteria in nosocomial infections that is resistant to treatment and control. The isolation of these bacteria is increasing significantly throughout the world. With increasing populations and a reduction in the use of synthetic drugs, many of these medicinal herbs are replacing other treatments..
The present study aims to investigate the inhibitory effects of Satureja khuzestaniea essence on the expression of the antibiotic resistance genes bla-OXA-23 and Housekeeping DNA gyrase-A in multidrug-resistant strains of Acinetobacter baumannii, using the RT-PCR technique..
Satureja khuzestaniea essential oil was collected from the Barij essence research farm in May 2010. Five multidrug-resistant strains of Acinetobacter baumannii that contain the bla-OXA-23 and housekeeping DNA gyrase-A genes were selected. The disk diffusion method was used to evaluate the antimicrobial effect of essential oil, and major components were used. The minimum inhibitory concentration (MIC) values of the Satureja khuzestaniea essential oil were determined via a broth macro dilution assay, as recommended by the CLSI. Finally, an analysis of the bla-OXA-23 and Housekeeping DNA gyrase-A was performed using the RT-PCR technique..
The major components of Satureja khuzestaniea essence are carvacrol (90.88%), ρ-cymene (3.11%), γ terpinene (1.24%), and linalool (0.91%). The RT-PCR technique demonstrates that Satureja khuzestaniea with an MIC of 0.2 μL/mL has the effect of reducing the expression of the antibiotic resistance gene bla-OXA-2. The essence has no inhibitory effect, however, on the Housekeeping DNA gyrase-A gene..
This study indicates that Satureja khuzestaniea essence has inhibitory effects on the gene expression of antibiotic resistance in bla-OXA-23 that has a high MIC. Given that this essence has a good inhibitory effect on gene expression in MDR Acinetobacter baumannii and bla-OXA-23, the results indicate that it could be used as a natural way to prevent the growth of Acinetobacter baumannii..

Infection due to Acinetobacter baumannii has become a significant challenge to modern healthcare systems. The rapid emergence and global dissemination of A. baumannii as a major nosocomial pathogen is remarkable and it demonstrates its successful adaptation to the 21st century hospital environment. Recent studies have discussed about essential oil of Origanum vulgare against a range of bacteria, including various species of Staphylococcus, Pseudomonas, Bacillus and Escherichia coli.. The present study aimed to investigate the inhibitory effects O. vulgare essence against multidrug-resistant (MDR) strains of A. baumannii from selected hospitals in Tehran, Iran.. This oil was obtained using the hydrodistillation method and analyzed by gas chromatography mass spectrography (GC/MS). The antimicrobial activity against MDR isolates was achieved using disc diffusion method and macro-broth dilution assay.. Analysis of the essential oil revealed the presence of pulegone (68.59%) piperitone (7.8%), piperitenone (7.8%), 1, 8-cineole (1.3%), and carvacrol (1.6%) as the major components. The results showed a significant activity against MDR A. baumannii with inhibition zones and minimal inhibitory concentration values in the ranges of 7-15 mm and 20-35 µL/mL respectively.. This investigation showed that the essence oil of O. vulgare had a potent antimicrobial activity against MDR A. baumannii. Further research is required to evaluate the practical values of therapeutic applications..

Significant left main coronary artery stenosis jeopardizes the entire myocardium of the left ventricle and has the worst prognosis of any form of coronary artery disease. Coronary-artery bypass grafting (CABG) has been considered as the standard therapeutic approach for such patients. There are limited data on the safety and effectiveness of percutaneous coronary intervention (PCI) in patients with unprotected left main coronary artery disease. In this study we have reported our experience on early, intermediate and long term results of LMC intervention. From Dec. 2007 to Mar. 2012, PCI with drug eluted stent (DES) or in some cases by a bare stent was performed on de-novo lesions of unprotected left main coronary artery in 50 patients. The inclusion criteria were: patients having refused CABG but with favorable anatomy for stenting; patients with poor general condition or comorbidity whom were refused by the cardiac surgeon and emergent patients for whom CABG was not accessible. The angiographic and procedural success rate was 100%. Four patients died, two because of severe heart failure, the third due to noncardiac etiology, and the fourth due to probable stent thrombosis. We have one target lesion revascularization (TLR=2%) and no reinfarction was occurred. Routine DES implantation in unprotected left main disease seems a feasible and safe method with favorable outcomes.