keyvan jamshidi

Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. For this purpose, the lungs of 402 goats, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of 26 goats (%6.46). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3% and 11.6%, respectively. Pneumonias were microscopically classified in goats as interstitial pneumonia (n=15) (57.69%), suppurative bronchopneumonia (n=4) (15.38%), bronchointerstitial pneumonia (n=3) (11.53%), and parasitic pneumonia (n=4) (15.38%). A total of 22 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of AdV antigen, were considered. AdV antigen was determined to be (13.63 %) (n=3) by the immunohistochemistry (IHC) method. In conclusion, the presence of viral antigen in lung tissues of goats may indicate that natural pneumonia may be induced by AdV or possibly other species-specific AdVs. Moreover, it is suggested that AdV might have a role in predisposing this species to secondary bacterial infections.

In the present study, in which the main goal was to determine the prevalence of RSV infection in goats slaughtered in Garmsar slaughterhouse, a total of 402 goat were subjected to postslaughter inspection. Among them, 67 (16.7%) cases were found having macrosdopic lesions of pneumonia particularly on the cranioventral lobs. Lesions were more frequently encountered in anteroventral lobes than caudal lobes. With the exception of 18 cases in which parasitic pneumonia and catarrhal and purulent bronchopneumonia were observed, the remaining 49 cases with macroscopic lesions of interstitial pneumonia and bronchointerstitial pneumonia used for RSV antigen detection. Formalin-fixed and paraffin-embedded lung tissue samples were immunohistochemically stained using routine avidin-biotin-peroxidase complex techniques to detect RSV antigens. In the present study, out of 49 cases with interstitial pneumonia and bronchointerstitial pneumonia, in 9 cases (18.36%) RSV viral antigens was detected, which were mainly found in the cytoplasm of bronchiolar epithelial cells, type II pneumocytes, and less frequently in the epithelial cells of bronchial glands, syncytial cells, alveolar macrophages, and lymphocytes and plasma cells. In conclusion, despite of the determining the prevalence of RSV viral antigens in pneumonic lungs of goats in the Garmsar region, a possible role of RSV in the induction of interstitial pneumonia in goats was suggested.