kourosh arzamani

Visceral leishmaniasis, a potentially fatal disease caused by Leishmania infantum, is prevalent in the Mediterranean region and the Middle East. In Iran, it is endemic in the Northwest, Southwest, Northeast, Southeast, and central regions, with sporadic occurrences elsewhere. This study aimed to ascertain the period prevalence, epidemiological characteristics, and the most common clinical symptoms and laboratory signs of visceral leishmaniasis in North Khorasan Province.

Over a nine-year period from 2010 to 2018, clinical records of visceral leishmaniasis cases were scrutinized to gather demographic and clinical data. Diagnosis was confirmed paraclinically through positive smears for Leishman bodies in bone marrow aspirates. Each patient completed a questionnaire.

A retrospective analysis was conducted on 24 diagnosed patients. Reported case numbers varied annually, with prevalence ranging from 0.0 to 1.1 per 100,000, peaking in 2017 and reaching its nadir in 2011. The majority of patients were hailed from rural areas (83.3%), with age distribution spanning from 8 months to 62 years. Most patients (95.8%) were under 10 years old, and 66.7% were male. Predominant clinical symptoms and laboratory signs included fever (91.7%), splenomegaly (79.2%), anemia (83.3%), thrombocytopenia (58.3%), and leukopenia (54.2%).

The incidence of visceral leishmaniasis in North Khorasan Province surpasses the national average by threefold, indicating its significance as an endemic hotspot for the disease in Iran.

Scientific overwhelming evidence confirms that the prevalence and incidence of mosquito-borne viruses such as chikungunya (CHIK) are dramatically increasing in Middle- Eastern countries including Iran.

I n t his r eview a rticle, u sing r elevant k eywords (“Chikungunya” OR “CHIKV” OR “Aedes albopictus” OR “Aedes aegypti”), available literature was searched to collect data related to the reports of CHIK and its main vectors, Aedes albopictus and Ae. aegypti, in Iran and 15 neighboring countries: Pakistan, Afghanistan, Turkmenistan, Russia, Azerbaijan, Armenia, Turkey, Iraq, Kuwait, Bahrain, Qatar, United Arab Emirates (UAE), Oman, Saudi Arabia, and Yemen.

CHIK was reported in neighboring countries such as Pakistan, Turkey, Russia, Saudi Arabia, Yemen, Oman, Qatar, and Iraq. Furthermore, presumably introduced in 2019, CHIK was reported in 11 provinces in Iran with overall seropositivity of 17.23% in humans over the past three years. The mosquitoes, Ae. albopictus and Ae. aegypti, have recently been reported in the southeast and south of Iran.

Given the change in climate, even if the density of the vectors is still limited, there is no guarantee that their population will not spread much more in the coming years. This emphasizes the urgent need to strengthen the surveillance system for the vectors, and sustained surveillance of CHIK infections in mosquitoes and humans, preferably in a collaborative international project.

One of the Arboviral diseases mostly transmitted by mosquitoes of the family Culicidae is Sindbis virus (SINV). Conduction of studies on the distribution of the distribution of vector-borne viruses and their prevalence has become very important in recent years. The present study aimed to investigate the status of this virus in Iran and adjacent countries by reviewing published literature.

Various databases including Magiran, IranDoc, Civilica, Jihad Daneshgahi Scientific Information Center (SID), Web of Science, Scopus, Science Direct, Google Scholar, and PubMed were investigated in both Persian and English languages.

The results indicated the evidence of the presence of the virus in Iran and eight neighboring countries. Evolutionary analysis and alignment of registered sequences in GenBank showed 100% similarity of sequences registered from Iran with a number of sequences registered from Armenia and Russia.

The current review of the studies and sequences registered in GenBank shows the presence of the virus in Iran and other countries in the region. So far, very few studies have been carried out on the epidemiology of this virus in Iran and the Middle East. Further studies in different parts of these regions are recommended.

Ticks are considered as the main vectors for the transmission of various pathogens such as relapsing fever and CCHF to humans. This study was investigated the biodiversity indices and medically importance of ticks in North Khorasan Province, Northeast of Iran during 2015–2019.

Specimens were captured from infested ruminants including cows, sheep, and goats. Additionally, tick col lections also were performed on non-domesticated creatures such as turtles, rodents, and hedgehogs. Specimens were identified using valid identification keys. Species diversity, species richness and evenness indices have been calculated to estimate species biodiversity of ticks.

A total of 1478 adult ticks were collected. The specimens were from two families: Ixodidae (90.05%) and Ar gasidae (9.95%), 6 genera and 17 species including: Rhipicephalus sanguineus (55.9%), Rhipicephalus bursa (13.4%), Hyalomma marginatum (9.5%), Hyalomma anatolicum (9.5%), Hyalomma asiaticum (0.2%), Hyalomma aegyptium (0.5%), Hyalomma scupense (1.3%), Hyalomma sp (1.2%), Haemaphysalis sulcata (0.7%), Haemaphysalis erinacea  (0.1%), Haemaphysalis inermis (0.1%), Haemaphysalis punctata  (0.2%), Haemaphysalis concinna (0.1% Boophilus annulatus (1.2), and Dermacentor marginatus (6.1%) among hard ticks as well as  Argas persicus (91.8%) and Argas reflexus (8.2%) amongst soft ticks. Rhipicephalus sanguineus, Rh. Bursa, Hy. marginatum and Hy. anatolicum were known as the most frequent species of hard ticks. Tick’s species richness, Shannon diversity index and Simpson index in this area were S= 17, H’= 1.69, D= 0.294 respectively.

Based on tick distribution veterinary authority, public health organizations and other officials should act for implementation of disease prevention.

Scorpion sting (SS) is an important public health hazard that may cause a potentially lethal condition especially in tropical and sub-tropical regions. Although scorpions are locally abundant in some parts of North Khorasan Province, SS has been poorly studied in this region. The current survey was carried out to investigate the epidemiological aspects of SS in North Khorasan Province, Iran.

This cross-sectional descriptive-analytic study collected and analyzed the data of SS cases admitted to medical centers of different counties in North Khorasan Province including Bojnurd, Shirvan, Esfarayen, Jajarm, and Raz from 2007 to 2018. The descriptive statistical analyses were carried out with SPSS software (version 24), and logistic regression analyses were performed using the STATA software.

During the study period, 540 SS cases were admitted to hospitals, of whom 44 persons were hospitalized, and nearly half of them were male (50.2%). Most SS cases occurred indoors (58.4%). The mean age of the patients was 35±18.0 years. Furthermore, the annual number of SS cases varied with an increase in 2013. The most common site of the sting was in the hands (41.8%) followed by feet (40.5%). The most common time for SS occurrence was 12 a.m. to 6 a.m. (38%). The majority of victims admitted to the hospital less than 3 hours after the sting (84.6%) and all of them were recovered. The results of multiple regression analysis indicated that the risk of hospitalization in people who had more than one bite was significantly higher than those who had been bitten once.

Given the low average number of SS cases reported in the area compared to the south of Iran, SS does not seem to be an important health issue in this area except for Jajarm County.

Phlebotominae sand flies (Diptera: Psychodidae) are the vectors of leishmaniasis. There are different methods for sand fly collection with different performance. The purpose of the current study was to compare the effect of different traps for collection of Phlebotominae sand flies in three endemic leishmaniasis foci in North Khorasan Prov ince, northeast of Iran.

Sand flies were collected using seven different traps from three villages, three times each twenty days during peak periods of seasonal activity in 2016.

A total of 7253 sand flies were collected. The specimens belonged to19 species. Phlebotomus sergenti was the most predominant species in the study area. Light trap baited with Carbon dioxide (CLT) and sticky paper trap (SPT) caught 22.6% and 22.3% of sand flies respectively. Animal baited trap (ABT) and white Shannon trap (WST) caught significantly fewer sand flies than the other traps. The sex ratio was different by phlebotominae sand fly species and collection methods. The sex ratio was highest in SPT and lowest in black Shannon trap (BST). Species diversity and species richness in SPT were more than other traps.

Our findings confirm that CLT and SPT are the most efficient sand fly collection methods. CLT is higher attractive for females and Phlebotomus genus and is an ideal method for monitoring the population of Phlebotomus ge nus during surveillance. SPT is an inexpensive, convenient and easy to be used to detect the presence of sand flies at low densities and provide a more realistic estimation of sand flies biodiversity.

Scorpions pose one of the most important public health and medical problems in tropical and subtropical regions of the world, especially in developing countries. This study was conducted to determine the fauna and spatial distribution of scorpions.

In this descriptive study, scorpions were captured using ultra-violet (UV) light, pitfall traps and digging methods in North Khorasan Province, northeastern Iran in 2017. After being encoded, the collected scorpions were stored in plastic containers of 70% ethanol and then transferred to the medical entomology lab of Tehran University of Medical Sciences for species identification based on morphological keys. In addition, Arc GIS 9.3 software was utilized for mapping spatial distribution of scorpions.

Overall, 143 scorpions were captured and identified. All of collected scorpions belonged only to Buthidae family. They were also classified into four genera (Androctonus, Mesobuthus, Odontobuthus, Orthochirus) and five species: M. eupeus (59.44%), A. crassicauda (16.78%), O. doriae (12.59), M. (Olivierus) caucasicus (9.09%), and O. farzanpayi (2.10%). Furthermore, spatial distribution of scorpions was performed in this area.

Regarding the diversity, high frequency and wide geographical distribution of scorpions and their longterm seasonal activity in this area, the probability of occurrence of scorpion sting is high. Therefore, in order to prevent the occurrence of this public health problem, health educational programs be implemented by health- care providers in the area.

The impact of environmental factors and host on Hyalomma spp. community structure and abundance in the main Crimean-Congo haemorrhagic fever (CCHF) foci of Iran is largely unknown.

Biotic and abiotic factors, including host, temperature, humidity, altitude, Köppen-Geiger climate types, sea son, and precipitation on Hyalomma spp. community structure and abundances in 11 provinces of Iran were investigat ed. Additionally, the possible infection of ticks with CCHF virus was evaluated using reverse transcription PCR tech nique.

Species analyses demonstrated the presence of Hyalomma anatolicum, H. marginatum, H. dromedarii, H. asi aticum, H. detritum and H. schulzei in the study area. Hyalomma anatolicum was the dominant species in the southern and northern parts, whereas H. dromedarii was distributed mostly in central parts of the country. The highest tick infestation was recognized in hot season. Spatial variation in tick relative density was observed between habitat types where more ticks were collected in deserts, semi-deserts, and Mediterranean habitats. Except for H. dromedarii, which was more preva lent on camel (P= 0.044), there were no significant variations in the frequencies of other Hyalomma species on different hosts. Hyalomma anatolicum, H. dromedarii frequencies had significant positive and negative asso ciation with tempera ture and precipitation respectively. Also humidity has positive impact on H. asiaticum frequency.

Data presented here will help improve ecological models to forecast the distribution of Hyalomma spp. ticks, to evaluate the risk of CCHF and other tick-borne diseases, and to design proper vector control measures to sup press Hyalomma populations in Iran.

No Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a prevalent tick-borne disease in different regions of Iran. This molecular and serologic study was performed to investigate the Crimean-Congo hemorrhagic fever virus (CCHFV) in collected ticks and in blood samples of some domestic animals in North Khorasan, Northeast of Iran.

In this cross sectional study, 136 blood samples from domestic animals (sheep, goats, and cows) collected in the Northeast region in Iran were examined using IgG ELISA assay. Ticks (n = 1478) were collected from sheep, goats, and cows. Out of all collected ticks, 62 specimens were investigated for CCHF virus genome using RT-PCR technique. The data were descriptively presented by median and 95% confidence interval (CI).

CCHFV infection rate was 8.1% in studied ticks. Two species of ticks, Hy. anatolicum (n=3; 15%, 95% CI 9.41–20.59) and Rh. sanguineus (n=2; 6.9%, 95%CI 4.33–8.58), were infected with CCHFV genome and were probable vectors of CCHF virus in the area. Infection rate was 15.4% for CCHFV in tested domestic animals. Serologic tests detected CCHFV specific IgG antibodies in 16.2% (95% CI 13.49–18.83) (99/16) and 19.2% (95% CI 13.26–25.20) (26/5) of sheep and goats, respectively.

The present study showed that domestic animals and ticks were infected with Crimean-Congo hemorrhagic fever virus and that the disease was endemic in North Khorasan province, Iran. However, further surveillance and prevention programs are recommended.

We aimed to determine the species composition, richness and diversity indices of the Phlebotomine sand flies at different topographic condition in visceral (VL) and cutaneous leishmaniasis (CL) foci in the northeast of Iran. This cross-sectional study was conducted during 2016 in North Khorasan Province. The sampling was focused on rural regions, where human cases of VL and/or CL were diagnosed and reported during last 5 years. Sand flies were collected three times each twenty days during peak periods of seasonal activity. Seven collection methods were used. Some Alpha and Beta diversity indices were calculated. Overall, 7253 sand flies were collected and identified. They were from 19 species of Phlebotominae sand flies. Phlebotomus sergenti and Ph. papatasi were the most prevalent (84.9%) species in the study area. Species rich ness (S) was very different in three areas and were18, 8, and 4 respectively but Evenness (E) were 0.357, 0.345, and 0.380, so evenness was almost equal in the study areas. Shannon Index (H ) and Margalef Richness Index were cal culated 1.033, 0.718, 0.527 and 2.117, 0.8998, 0.4006 respectively. The sand fly fauna in North Khorasan Province was very rich and often included some of the most im portant proven or suspected vectors of leishmaniasis. Species diversity indices (Shannon index, and Simpson’s in dex) were not high due to decreasing in evenness. The Margalef richness index could accurately reflect the biodiver sity of sand flies between three subtidal locations.

Rodents are one of the most important hosts for some zoonotic diseases and also act as a reservoir of some ectoparasites and endoparasites. They cause damage to the farms and inflict public health. The aim of this study was to investigate the faunal composition of rodent in North Khorasan Province, Northeast of Iran.
The sampling was carried out between 2011 to 2013. The specimens were collected using different methods including rodent death and live traps, digging of their burrow, and hand net from 75 different sample sites.
The total number of 396 specimens belonging to 22 species and six families were identified. The results illustrated the high numbers and densities of Meriones persicus (17.68%), Meriones libycus (15.15%), Nesokia indica (7.32%) and Rhombomys opimus (6.82%), as the most important reservoirs for different zoonotic diseases. Moreover, significant number of other rodent species including Mus musculus (15.66%), Apodemus witherbyi (13.89%), A. hyrcanicus (0.25%), Rattus norvegicus (1.01%), Meriones crassus (0.25%), Gerbillus nanus (0.51%), Microtus paradoxus (2.27%), M. transcaspicus (0.76%), Ellobius fuscocapillus (0.25%), Cricetulus migratorius (4.29%), Calomyscus elburzensis (4.29%), C. mystax (1.26%), Spermophilus fulvus (0.25%), Dryomys nitedula (3.54%), Allactaga elater (3.54%), Jaculus blanfordi (0.25%), Meriones zarudnyi (0.25%), M. meridianus (0.51%), and Hystrix indica as hosts for parasites and zoonotic diseases were identified.
The high biodiversity including at least 22 species and six families of rodents were found in North Khorasan Province, some of them were medically important species.

Rodents are an important source of zoonotic diseases for human. The aim of this study was to determine the infectivity of rodents with intestinal helminths in North Khorasan Province, Iran.
One hundred and thirteen rodents were collected using different collection methods such as kill and live traps, digging of their burrow, filling of their hiding places with water and hand net during 2011-2013. Their alimentary canals were removed in the laboratory and helminths were determined in the department of parasitology, Tehran University of Medical Sciences.
Thirteen species of helminths parasites were found in 13 species of rodents, including Aspiculuris tetraptera, Hymenolepis diminuta, Nippostrongylus brasiliensis, Protospirura Seurat, Rictolaria ratti, Skrjabinitaenia lobata, Streptopharagus kuntzi, Syphacia obvelata, Taenia taeniaeformis, Trichuris muris, Cysticercus fasciolaris, Acanthocephal. spp and Trichuris spp. Some of them were reported for the first time in new host in Iran. S. obvelata and A. tetraptera were the most frequent parasites and P. Seurat, R. ratti and C. fasciolaris were found only in one rodent.
This is the first study to investigate the intestinal parasites in rodents in this area. Among different species identified, some of helminths were reported in new host.

Dog can represent as an important source of zoonotic disease and important health problem for human. They can carry dangerous parasitic diseases such as hydatidosis, toxocariasis and Coenurus cerebralis to humans and animals. This study was performed in order to determine the prevalence and intensity of zoonotic parasites among stray dogs from Bojnurd, the capital city of North Khorasan province in North West of Iran. During a program performing by Bojnurd municipal on the slow killing of stray dogs, 32 dogs from Jun 2013 till March 2015 were selected. At necropsy their alimentary canals were removed and to identify the species of helminthes, the nematodes were cleared in lactophenol and cestodes were stained using carmine acid. Intestinal protozoan parasites were detected with parasitological methods. 28 (87.5%) of 32 stray dogs infected at least with one helminth. Seven species of cestodes were isolated from examined dogs and three species of nematode were detected. Giardia sp. and Cryptosporidium sp. detected from fecal samples. This is the first study of the prevalence of intestinal zoonotic parasites in dogs in this area. It seems control of bearing stray dogs can help human health and reduction economic losses caused by stray dog’s zoonotic parasites.

Although many studies had been conducted on various aspects of canine visceral leishmaniasis (CVL) in domestic dogs in the endemic areas of Iran, investigations on CVL in wild canines are rare.
This is a cross-sectional study was conducted from December 2012 to 2013 in northeast of Iran where human VL is endemic. Wild canines were trapped around the areas where human VL cases had been previously identified. Wild canines were collected and examined both clinically and serologically using direct agglutination test (DAT). Microscopically examinations were performed in all the seropositive wild canines for the presence of the amastigote form of Leishmania spp. Some Leishmania sp. which had been isolated from the spleens of wild canines, were examined analyzed by conventional PCR and sequencing techniques using α-tubulin and GAPDH genes.
Altogether, 84 wild canines including foxes (Vulpes vulpes, n=21), Jackals (Canis aureus, n=60) and wolves (Canis lupus, n=3) were collected. Four foxes and seven jackals showed anti-Leishmania infantum antibodies with titers of 1:320–1:20480 in DAT. Furthermore, one fox and one jackal were parasitologically (microscopy and culture) positive and L. infantum was confirmed by sequence analysis.
The present study showed that sylvatic cycle of L. infantum had been established in the studied endemic areas of VL in northeastern Iran.

Insects with over 30,000 aquatic species are known as very successful arthropods in freshwater habitats. Some of them are applied as biological indicators for water quality control, as well as the main food supply for fishes and amphibians. The faunistic studies are the basic step in entomological researches; the current study was carried out emphasizing on the fauna of aquatic insects in Karaj River, northern Iran. A field study was carried out in six various sampling site of Karaj River during spring 2013. The aquatic insects were collected using several methods such as D-frame nets, dipping and direct search on river floor stones. Specimens were collected and preserved in Ethanol and identified by standard identification keys. Totally, 211 samples were collected belonging to three orders; Plecoptera, Trichoptera and Ephemeroptera. Seven genuses (Perla, Isoperla, Hydropsyche, Cheumatopsyche, Baetis, Heptagenia and Maccafferium) from five families (Perlidae, Perlodidae, Hydropsychidae, Batidae, Heptagenidae) were identified. The most predominant order was Plecoptera followed by Trichoptera. Karaj River is a main and important river, which provides almost all of water of Karaj dam. So, identification of aquatic species which exist in this river is vital and further studies about systematic and ecological investigations should be performed. Also, monitoring of aquatic biota by trained health personnel can be a critical step to describe water quality in this river. Understanding the fauna of aquatic insects will provide a clue for possible biological control of medically important aquatic insects such as Anopheles as the malaria vectors.

Ticks of the order Ixodida are among the most proficient ectoparasites worldwide. Haemaphysalis is the second largest genus within Ixodidae. Southeast Asia is thought to be the original center of development and dispersal of the genus. Six species of Haemaphysalis, H. concinna, H. erinacei, H. inermis, H. parva, H. punctata and H. sulcata, have been recorded as occurring in Iran. The current paper deals with notes on taxonomy of H. erinacei and provides a provisional key for the identification of Haemaphysalis species that occur in Iran.