فهرست مطالب kumars amini

Cervical cancer is the second most common cancer among women. Interleukin-8 (IL-8) is a member of the chemokine family and is involved in initiating inflammatory reactions. Previous studies have shown that polymorphisms in the IL-8 gene are associated with an increased risk of various types of cancer. This study was performed with aim to investigate the association between IL-8 -251A> T polymorphism (rs4073) with cervical cancer in the female population in Tehran using Tetra primer arms-PCR.

In this case-control study which was performed in 2018, a total of 100 blood samples were examined, of which 50 samples were related to women with cervical cancer and another 50 samples belonged to healthy women as a control group. After DNA extraction from all samples, the frequency of IL-8 -251A> T polymorphism (rs4073) was evaluated using Tetra primer arms-PCR technique. Data were analyzed by SPSS statistical software (version 21) and chi-square test. P<0.05 was considered statistically significant.

The frequency of TT, AT, AA genotypes was 42%, 50% and 8%, respectively in the patient group and 24%, 70% and 6%, respectively in the control group. The frequency of A and T alleles in the patients was 33% and 67% and in the healthy individuals was 41% and 59%, respectively. There was no significant difference between the control and patient groups in allelic distribution (P = 0.24) and genotype in all three codominant models (p=0.16), dominant (p=0.055) and recessive (P = 0.6).

There was no relationship between IL-8 gene T-251A polymorphism and cervical cancer in the population of women studied in Tehran.

Periodontitis is an inflammatory multifactorial disease in oral tissues and many genetic reasons and environmental factors responsible. Vitamin D deficiency has been determined to be related to periodontal disease. This aim of this study was to investigate the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in 100 patients (as patient and control groups).

Blood samples from 50 patients and 50 control groups were selected and DNA from the samples was extracted by the DNA extraction kit. Genotyping was used by Tetra Arms-PCR method. The use of sequencing was confirmed by the Tetra Arms-PCR genotyping results. Then, statistical analysis was performed using SPSS statistics 20 software and T-test.

Frequency of AA, AC, and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in patients’ cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between the patient and control groups. Statistical analysis showed no significant association between this type of polymorphism and periodontitis disease in the studied samples (P=0.67).

This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the studied samples. To confirm the results of this study, further studies with large sample size and different types of population are recommended.

Periodontitis is one of the most common causes of damage to the gums and retaining structures of the teeth. Matrix protein, a metalloproteinase, is known as intermediate collagenase and the enzyme collagenase fibroblast, which is encoded in humans by the MMP-1 gene. The aim of this study was to investigate 1G/2G polymorphism in the MMP-1 gene and its association with the periodontal disease in the Iranian patients.

In this study, 50 patients with periodontal disease and 50 patients were selected as the control group in Kerman city. DNA was extracted from a person's blood sample using a kit. The desired primers were controlled by the NCBI site. Then, with the Tetra arms PCR technique, the desired polymorphism was multiplied. In the next step, the samples were transferred to electrophoresis gel and examined. The results were analyzed with SPSS software using T-test or Squer Chi-(X2).

The percentage of 1G/1G genotype in the patients group was 8% and for the controls group was 0%. The percentage of 2G/2G genotype for the patients group was 24% and for the controls group was 18%. In addition, the 1G/2G genotype frequency was 68% for the patients group, and for the controls group was 82% (P=0.83). The results of Tetra-arms PCR genotyping of the samples were confirmed by sequencing.

The findings indicated that in Iranian patients, MMP-1 -1607 1G/2G (rs1799750) was not significantly associated with periodontal disease. It is recommended to take more samples from different parts of Iran to confirm the results.

Mycoplasma are from cell culture the main polluters. Mycoplasma hominis is one of the most important human mycoplasmas are associated with infections of the genitourinary system in women and men. The aim of this study compare methods of detection of mycoplasma contamination in the genital secretions of men and women referring to infertility center is Kerman.

This descriptive cross-sectional study was performed on 100 women and 100 infertile men referred to Kerman Infertility Center during 6 months. Samples that were sent to the laboratory in the PPLO Broth filter 0.45 micrometers were passed and in accordance with the instructions, they were cultured in PPLO solid liquid and solid media and stored at 37 ° C, Co2 incubator. After the extraction of DNA by using PCR method and especial primers, the species and genus of Mycoplasma hominis were determined and studied.
Findings: Of the 200 samples examined by culture method, 17.5% of mycoplasma colonies were positive and using biochemical tests were mycoplasma hominis 6%. By PCR method, 44% of samples were infected with mycoplasma and 16.5% contaminated they were mycoplasma hominis.
Discussion &

The results of this study show that PCR is a sensitive and rapid method for detecting Mycoplasma huminis in infertile women and men