فهرست مطالب mahboubeh eslami moghadam

In a new approach, following the development in metal oxide chemistry, the ibuprofen as low water soluble nonsteroidal anti-inflammatory drug diffused into synthetic sol-gel derived nano porous g-alumina by an impregnation method in order to increase the solubility and control the drug release in physiological environment. Sol-gel method was utilized for the fabrication of alumina by controlled hydrolysis of an aluminum alkoxide source. This vehicle favors high surface area, pore diameter and pore volume as well as hydroxyl rich surface which is needed for the drug formulation. Two different percent of the medication were loaded on the synthetic g-alumina. The samples were characterized by X-ray diffraction (XRD),BET (Brunauer, Emmett and Teller), FT-IR and thermogravimetric analysis (TGA). The results showed that the drug molecules were well-distributed into the pores. 25 and 50% w/w of ibuprofen were prepared for drug release test which was studied by UV-Vis techniques. The release kinetic was obtained in simulated body fluid (SBF), simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). The solubility of the drug reached to 90 and 84% for 25% (γ-Al-IBU25) and 50% (γ-Al-IBU50) drug loaded samples after 4 h of loading time, respectively. These results are comparable to reported commercial alumina with low amount of 25% release. The percent of the drug release is as follow for three environments: SBF > SIF > SGF. It could be concluded that the new formulation led to enhancement solubility and controlled release of ibuprofen in the mentioned media.

Catalase is a basic antioxidant enzyme that exits in human organs, mainly in the liver. The liver as a main tissue in the body that plays a substantial role in the catabolism and detoxification of drugs is a target of toxic and carcinogenic effects of many drugs. In the present study, the side effects of an anti-cancer compound of oxali-palladium on the function and structure of liver catalase were investigated. In this experimental study, changes in the enzyme activity were studied by conversion in substrate (hydrogen peroxide) absorption at wavelength of 240 nm, using UV-visible spectroscopy in the absence and presence of different concentrations of oxali-palladium at room temperature. Furthermore, the effect of oxali-palladium on the tertiary structure of catalase was investigated using fluorescence spectroscopy via studying the changes in the intrinsic enzyme emission in the absence and presence of different concentrations of oxali-palladiumat at both room and physiologic temperatures. Kinetics data showed that the Km value of bovine liver catalase was 26.8 mM. Moreover, in the presence of different concentrations of oxali-palladium‚ the enzyme activity showed a gradual decrease in a dose-dependent manner (p<0.001). Fluorescence data presented changes in the tertiary structure of the enzyme by quenching fluorescence emission‚ that indicated alteration in protein chromophore environment. It could be concluded that inhibition of catalase enzyme by anticancer drug of oxali-palladium increases the content of reactive oxygen species. Increase in reactive oxygen species values is one of the chief mechanisms of different anticancer drugs.