فهرست مطالب mahmoud reza arefian

The biologic behavior and histopathological features of fibromatosis are intermediate between those of fibroma and fibrosarcoma.

The aim of the present study was to determine useful histopathologic and immunohistochemical characteristics for the differentiation of these lesions.

In this cross-sectional descriptive study, 40 specimens comprising 20 fibrosarcoma and 20 fibromatosis biopsies were selected. The histopathologic characteristics of these lesions were identified and immunohistochemical staining for Ki67 and β-catenin markers was performed. Sections were examined by light microscopy and positively stained cells were counted. Results were analyzed by SPSS 20 using Chi-square test, Mann-whitney test, and t-test (p< 0.05).

Statistical significant differences were observed between fibromatosis and fibrosarcoma in terms of distribution frequency, mitotic rate, herringbone pattern, cellularity, overlapping of nuclei, mean Ki67 score, and atypia rate. The other features including age, gender, necrosis, clarity of nucleoli and mean β-catenin were not significantly different.

The present findings suggest the mitotic figures, Ki67, herringbone pattern, cellularity, and atypia are useful to differentiate fibromatosis from fibrosarcoma.

Fibromatosis includes a variety of fibroblastic proliferation whose biological trend and histopathological patterns are at intermediate level between benign fibroblastic lesions and fibrosarcoma. Accordingly, because of overlapping of histopathologic features of fibrosarcoma, particularly low-grade type, with fibromatosis, the present study was conducted to find more precise criteria for histopathological and immunohistochemical (IHC) differentiation of these lesions. In this cross-sectional descriptive analytical study, a total of 40 specimens from pathology department archives in hospitals of Isfahan and Tehran universities from 2003 to 2013, including 20 fibrosarcoma and 20 fibromatosis biopsies, were selected. First, histopathologic characteristics were identified using H&E slides and an optical microscope H&E slides and then they were stained through immunohistochemical staining technique using the EnVision for markers Ki-67 and β-catenin. Afterward the samples were examined by optical microscope and positively stained cells were counted. There was no significant difference between fibromatosis and fibrosarcoma in terms of a mean age (P=0.063), distribution of gender frequency (P=0.197), necrotic rate (P=0.602), clarity of nucleolus (P=0.799) and SID mean of β-catenin marker (0.369). However, it was seen a meaningful difference between fibromatosis and fibrosarcoma in terms of frequency distribution (P=0.017), rate of mitotic figures (P<0.001), rate of herring-bone pattern (P=0.043), rate of cellularity (P<0.001), rate of nucleus overlapping (P<0.001), mean of Ki-67 (P=0.046), mean of Ki-67-limit (P=0.001) and atypia rate (P<0.001). There was a meaningful difference between fibrosarcoma and fibromatosis in terms of mitotic figures, expression of Ki-67 mitotic marker, herring bone pattern, cellularity and atypia. Therefore these features can be used to differentiate the relevant pathological lesions. However, no meaningful difference between two tumors in terms of expression and intensity of β-catenin, clarity of nucleoli and necrosis. This indicates that they are not reliable criteria of differentiation between fibrosarcoma and fibromatosis.

Periapical diseases are the final outcome of untreated pulpitis or failure of root canal treatment, collectively referred to as periapical lesions. Pulpitis is the final consequence of advanced dental caries or severe dental trauma, affecting the pulp. Spread of odontogenic infections into adjacent oral tissues and distinct structures by blood and lymph vessels can lead to more serious complications. Therefore, early and correct diagnosis and treatment of these lesions is necessary to prevent their potential complications. Considering the importance and high prevalence of periapical lesions, this study was undertaken to evaluate the periapical inflammatory lesions and spread of odontogenic infections. In this study, studies performed from 2000 to 2013 about clinical, radiographic, and histopathologic features and treatment of periapical lesions, and also spread of odontogenic infections were evaluated by running a search in PubMed, EBSCO, ISC and Google Scholar databases. Periapical periodontitis is one of the most common odontogenic diseases; the chronic type of it or periapical granuloma is more prevalent than its acute type or periapical abscess. Radicular cyst, the most common odontogenic cyst, is a sequela of periapical granuloma. Inflammatory lesions with an odontogenic origin can spread to the bone marrow, causing osteomyelitis. In addition, these lesions give rise to acute and serious diseases such as soft tissue abscesses and cellulitis if they are not managed on time. Early diagnosis of periapical lesions leads to easier management, more successful treatment and better prognosis.