فهرست مطالب mahnaz ahrabi

Exposure to pulsed electromagnetic field (PEMF) has been revealed to affect the differentiation and proliferation of human mesenchymal stem cells derived from dental pulp multipotent stromal stem cells (DP -MSCs). This study aimed to investigate the differentiation effect of electromagnetic fields (EMFs) on the DP-MSC.

PEMF was produced by a system comprising a multi -meter autotransformer, solenoid coils, and teslameter. This study included 10 groups of DP-MSCs which underwent different electromagnetic radiation time and beam intensity. Three samples tested for each group. The effect of PEMF with the intensity of 0.5 and 1 mT (mili Tesla) and 50 Hz on the proliferation rate of DP-MSC was evaluated at 20 and 40 minutes per day for seven days. MTT assay was applied to determine the growth and proliferation of DP-MSC. Gene expression of DMP1 for differentia tion of DPSCs to odontoblasts was confirmed by Real Time PCR., ANOVA statistical analysis and Kruskal-Wallis test were used to analyze the data.

The survival in all exposure groups was significantly higher than that in control except in the group of 40 minutes, 1 mT (P<0.05). In 20 minutes, 0.5 mT exposure, the survival intensity is significantly more than others (P<0.05). In general, the intensity of survival was recorded, 20, 0.5 mT ≥20, 1 mT ≥40, 0.5 mT≥40, 1 mT respectively. Therefore, according to the obtained results, ELF-EMF increases the survival of cells except for one case (40 minutes, 1mT), even though the effective underlying mechanisms in this process are still unclear.

The results obtained promise that in the future, by placing an important part of the pulp next to the electromagnetic field, the lost part of the pulp can be reconstructed and the dentin barrier can be created.

Chronic and progressive damage to the kidney by inflammatory processes, may lead to an increase in the extracellular matrix production, a condition known as renal fibrosis. The current study aims to evaluate if the extracellular vesicles (EVs) derived from autophagic adipose-derived mesenchymal stem cells (ADMSCs) can reduce the inflammation and extracellular matrix accumulation in damaged kidney tissue.

Autophagy was induced in ADMSCs using 2μM concentration curcumin and was confirmed by evaluating LC3B, ATG7, and Beclin1 using real-time polymerase chain reaction (PCR) and Western blot. An in vitro renal fibrotic model was established in HEK-293 cells exposed to H2O2 (0.8mM) for 24 and 72 hours. The fibrotic model was confirmed through evaluation of collagen I, transforming growth factor-beta 1 (TGF-β1), E-cadherin, and vimentin genes expression using real-time PCR, collagen I protein by ELISA. After induction of fibrosis for 24 and 72 hours, the HEK cells were treated with NEVs (non-autophagy EVs) (50μM) or AEVs (autophagy EVs) (50μM) at 48, 96, and 124 hours, and then the samples were collected at 72 and 148 hours. Expression of collagen I, TGF-β1, E-cadherin, and vimentin Genes was evaluated via RT-PCR, and protein levels of IL1, TNF-α, IL4, IL10 using ELISA.

Induction of autophagy using curcumin (2μM) for 24 hours significantly increased LC3B, Beclin1, and ATG7 in the ADMSCs. Upregulation in anti-fibrotic (E-cadherin) and antiinflammatory (IL4, IL10) gene expression was significantly different in the fibrotic model treated by AEVs compared to NEVs. Also, the downregulation of fibrotic (TGF-β1, vimentin, collagen I) and pro-inflammatory (IL1, TNFα) gene expression was significantly different in AEVs compared with those treated by NEVs.

Our findings suggest that AEVs can be considered as a therapeutic modality for renal fibrosis in the future.

Parkinson’s disease (PD) is a progressive and severe neurodegenerative disorder of the central nervous system (CNS). The most prominent features of this disease are cell reduction in the substantia nigra and accumulation of α-synuclein, especially in the brainstem, spinal cord, and cortical areas. In addition to drug-based treatment, other therapies such as surgery, cell therapy, and laser therapy can be considered. In this study, articles on cell therapy and laser therapy for PD have been collected to evaluate the improvement of motor function, cell differentiation, and dopaminergic cell proliferation.

Articles were collected from four electronic databases: PubMed, Scopus, Google Scholar, and Web of Science from 2010 to 2022. The keywords were “photobiomodulation”, “low-level light therapy”, “Low-level laser therapy”, “near-infrared light”, “Parkinson’s disease”, “Parkinsonism”, and “stem cell therapy”. About 100 related articles were included in the study.

The results of the studies showed that cell therapy and laser therapy are useful in the treatment of PD, and despite their limitations, they can be useful in improving PD.

Concomitant use of cell therapy and photobiomodulation therapy can improve the symptoms of PD

Glass ionomer and polycarboxylate cement have different effects on the marginal seal, microleakage, pulp tissue stimulation, and gingival health. The purpose of this study was to assess the effect of these cement on the gingival health of primary molars restored with stainless steel crowns (SSC).

A total number of 34 children were selected who were within the age range of 4-7 years and required SSCs on both sides. The selected teeth were identical in terms of the dental arch and tooth number. After preparing the teeth, glass ionomer and polycarboxylate were used randomly on each side to cement SSCs. After placing the crowns, parents were asked to maintain the oral hygiene of their children by brushing and flossing their teeth. Subsequently, 6 months after the crown cementation, the gingival index, plaque index, and additional cement were evaluated. Statistical analysis was performed in SPSS software (version 25) using Wilcoxon Rank, Chi-square, and binary logistic regression tests.

There was more gingival inflammation in the group of teeth that used polycarboxylate as cement (P=0.022) and in the lower arch (P=0.007). The plaque index was significantly lower 6 months after the crown cementation (P<0.001).

Based on the results, gingivitis is less prevalent in primary molars with SSCs cemented with glass ionomer. Moreover, maxillary primary molars have a lower rate of gingivitis after placing SSCs. Besides, gender and tooth numbers did not affect the gingival health of primary molars restored with SSC.