فهرست مطالب mahsa emami

Medicinal plants contain valuable active compounds that are produced in limited quantities under natural conditions, so the use of methods such as tissue culture and stimulant materials is useful to produce more secondary metabolites in medicinal plants. In this study, the effects of methyl jasmonate and LED lights as two elicitors on physiologic and secondary metabolites in the roots of 30-day-old licorice seedlings in MS media were investigated. In this study, concentrations of zero, 0.2, 0.5, 1 and 2 μM methyl jasmonate as well as blue, red and blue+red light treatments, were used in three replications in a completely randomized design. The results showed that flavonoid content at wavelengths of 300 nm at a concentration of 1 μM methyljasmonate showed a significant increase compared to the control. According to results, the amount of phenolic compounds in methyl jasmonate treatment increased at a concentration of 1 μM and in blue light treatment. The highest activity of phenylalanine ammonialyase was observed in blue light, but the greatest amount of glycyrrhizin (12.10 µg/g FW) was recognized in red light treatment. Also, red light significantly increased the growth and proliferation of plants compared to other treatments. In general, it can be concluded that the use of stimulants such as methyl jasmonate and red and blue lights increases the amount of secondary metabolites in medicinal plants.

‘Yaghoot’ grape cultivar is the earliest cultivar known in the history of Iran. The main problem of this cultivar is the compactness and small size of its berries, under the influenced genes that are controlled mainly by Gibberellic Acid (GA3). Those undesirable traits reduce customer interest and profitability. To alleviate these problems, we used GA3 and carried out Girdling (G) to enhance the quantitative and qualitative characteristics. The GA3 treatment was used at four concentrations, i.e.: 0, 60, 90, and 120 mg/l, and was sprayed on plants three times (i.e.: 10 days before flowering, in the midst, and after flowering). The G treatment has achieved the proximity of the branch bases during the formation of berries. In this experiment, various morphological and biochemical traits were measured. The results exhibited that the GA3 and G treatments significantly positively affected the fruits' quantitative and qualitative characteristics. Both treatments were observed to increase the berry length, berry weight, berry diameter, berry width, cluster width, cluster length, cluster weight, titratable acidity, total soluble solids, proline, malondialdehyde, H2O2 content, total phenolic content, anthocyanin content, ascorbate content, flavonoids content, antioxidant capacity, catalase activity, peroxidase activity, polyphenol oxidase activity, superoxide dismutase activity, and ascorbate peroxidase activity. The interaction between the GA3 and G showed that they significantly increased the berry weight, proline content, antioxidant capacity, ascorbate content, peroxidase activity, polyphenol oxidase activity, ascorbate peroxidase activity, catalase activity, and superoxide dismutase activity. Those treatments are promising treatments that can help improve the quantitative and qualitative characteristics of ‘Yaghoot’ grapes.