فهرست مطالب marjan nikbakht zadeh

Central nervous system demyelination is the main feature of multiple sclerosis (MS).  The most important unmet need in MS is use of treatments that delay the progression of the disease. Leucine-rich repeat and Immunoglobulin-like domain containing NOGO receptor-interacting protein 1(LINGO-1) have been known as inhibitors of oligodendrocyte differentiation and myelination. We investigated LINGO-1 antibody effects on remyelination and neurobehavioral deficit using cuprizone-induced demyelination. Animals were randomly divided into three groups (n = 10): (1) Control group; received the regular diet, (2) CPZ group; normal saline was injected intraperitoneally, and (3) Treatment group; LINGO-1 antibody (10 mg/kg) was injected IP once every six days for 3 weeks. We assessed the level of myelin basic protein (MBP), neurofilament heavy chain (NF200), and Brain-derived neuroprotective factor (BDNF) in the corpus callosum (CC) by immunostaining against MBP, NF200, and BDNF. We found  decreased levels of MBP, NF200, and BDNF in demyelinated CC, and anti-LINGO-1 treatment improved demyelinated structures. Furthermore, motor impairment was measured by Open-field (OFT) and Balance beam tests. In the treatment group, motor impairment was significantly improved. These results provide evidence that LINGO-1 antibody can improve remyelination and neurobehavioral deficit.

An inappropriate lifestyle and nutritional habits such as high fat, high calorie food intake and quality of drinking water can change sperm parameters. Despite the fact that there are different and sometimes contradictory beliefs about the suitable temperature for drinking water, unfortunately, there is not much proven evidence. There are some long-term adverse effects of cold-water intake like liver, gastrointestinal and other internal organ damages and dysfunction.  Regarding the high consumption of ice-cold water as a beverage and its probable role for infertility based on some trivial evidence and clinical senses, evaluation of the effects of ice-cold water drinking on reproductive system is noteworthy. Therefore, the main aim of this study is to assess the effect of long-term drinking of ice-cold water on the sperm count, morphology and motility and testis histology. Twenty-four male Wistar rats randomly divided into four groups (n = 6):  Intervention groups that used ice-cold water (4oC) for two months (CW2M), three months (CW3M), two months ice-cold water and then one-month room-temperature water (CW2M + 1MRW), and control group that used room-temperature water. Spermatogram and histology of testis were assessed. Ice-cold water drink for the long run, reduced sperm count and sperm progressive motility; increased the percentage of non-motile sperm; changed normal morphology of sperm and destroyed Sertoli and Leydig cells. In conclusion, long-term ice-cold water drinking ought to be noxious for testis function and structure.

Drinking ice-cold water is prohibited in Avicenna’s “The Canon of Medicine” book in which he emphasized that ice-cold water drinking was hazardous for the body organs such as the liver. Little information can be found regarding the effects of ice-cold fluid drinks on liver and its probable sequels on this vital organ. Accordingly, we investigated the effects of long-term ice-cold water drink on the rat liver function and histology. Eighteen male Wistar rats, weighing 180±20 g, were randomly divided into three groups of six as two months ice-cold water drink, CW2M; three months ice-cold water drink, CW3M; and three months room temperature water drink; control group. Upon completion of the care period, a blood sample has been taken for liver enzymes and lipid profile assessment. Liver tissue has also been used for histological studies of H&E staining and microscopic examination. Histological findings showed hepatocellular micro-vesicle formation, necrosis and derangement of the cellular cords and infiltration of Kupffer cells in ice-cold water taken animals. Serum TGs, VLDL-C and ALP significantly increased with sound decrease in FBS and LDL-C in ice-cold water taken animals. It seems that long-term ice-cold water has deleterious functional and structural effects on the liver.

Post-traumatic stress disorder (PTSD) symptoms and pain can be appeared repeatedly following traumatic events. Although in acute stress condition, pain perception significantly covered by stress, few studies have discussed the changes in pain perception in a PTSD stress conditioning. In the present study, changes of facial acute pain and allodynia have been investigated in an animal model of PTSD.

Male Wistar rats were divided to two groups as control and under stress. PTSD was induced by single prolonged stress (SPS) method and confirmed by elevated plus maze (EPM) and rats plasma corticostrone levels. Acute facial pain and allodynia were evaluated by the eye wiping test and von frey test in the experimental groups.

SPS rats spent less time and made a fewer entries into the open arms of EPM (p < 0.05). The corticostrone level was significantly decreased in Stress + DEXA group compared to the Control + DEXA (p < 0.05). The number of eye wiping was significantly decreased in SPS-induced rats compared to the control (p < 0.001). The response threshold of applying von frey filament in face of SPS-induced rats was significantly increased compared to the control (p < 0.001).

Acute pain perception and allodynia of face area of rats was reduced in SPS-induced animals. Increased endogenous opioids in PTSD condition may lead to this observation.