maryam meskini

In cystic fibrosis patients, the mucus is an excellent place for opportunistic bacteria and pathogens to cover. Chronic infections of upper and lower airways play a critical role in the mortality of cystic fibrosis. This study aimed to introduce the microbiota profiles in patients with cystic fibrosis.

In this study, a comprehensive literature search was done for studies on upper and lower airway microbiota in cystic fibrosis patients. International and national databases were searched for the following MeSH words: microbiota, microbiome, upper airway, lower airway, cystic fibrosis, cystic fibrosis, upper airway microbiome, lower airway microbiome, microbiome pattern in cystic fibrosis, microbiome pattern in cystic fibrosis, upper airway microbiota, lower airway microbiota, and microbiota pattern.

Streptococcus spp. are in significantly higher relative abundance in infants and children with cystic fibrosis; however, Pseudomonas spp. are in higher relative abundance in adults with cystic fibrosis. Molecular diagnostic techniques can be remarkably accurate in detecting microbial strains.

For the detection and isolation of most bacterial species, independent-culture methods in addition to the standard culture method are recommended, and sampling should include both upper and lower airways.

The use of antibiotics is common, but its excessive or improper use leads to antibiotic resistance. Consequently, scientists have been interested in exploring traditional medicine to identify a new source of anti-bacterial agents. The present study aimed to conduct a systematic review to determine the anti-bacterial activity of Satureja khuzestanica Jamzad (SKJ) and Zataria multiflora Boiss (ZM) essential oils against Pseudomonas aeruginosa in vitro.

A comprehensive literature search for studies published on SKJ and ZM was carried out. Various keywords (S. Khuzestanica, Marzeh-e-Khuzestani, Z. Multiflora, Z. Multiflora Boiss; avishan-e-shirazi, shirazi thyme, thymol, carvacrol; P. aeruginosa, Iran, antibacterial effect; traditional medicine, phytomedicine, herbal medicine) were used to search both international and Iranian databases (Google Scholar, Science Direct, Web of Science, MEDLINE; PubMed, Scopus, Cochrane Library, Academic Search; Journal Storage, Magiran, Irandoc, Scientific Information Database (SID), and IranMedex). The selected articles were published during 2000-2017 and were written in English or Persian.

Seventeen articles were included in the review. The main ingredients of SKJ and ZM plants were carvacrol and thymol. The potential anti-bacterial activity of essential oils from these plants was confirmed. The carvacrol content, as the major active ingredient of SKJ was between 38.33-97.89%. The major ingredients of ZM were carvacrol (16.8-82.7%) followed by thymol (25.70-64.87%). The minimum inhibitory concentration (MIC) value of SKJ and ZM essential oils against bacterial strains was in the range of 0.31-450 and 2-8,000 μg/mL, respectively.

The present review study confirmed the anti-bacterial activity of SKJ and ZM, particularly against Pseudomonas in vitro.

In recent years, the risk of acquiring antibiotic resistance has been increasing due to the widespread use of antibiotics. This study aims to evaluate the antibiotic resistance pattern in patients referred to Ali-ebn Abi-Taleb hospital.

In a descriptive study, samples of urine, blood, cerebrospinal fluid, and body fluid were collected from patients referred to Ali-ebn Abi-Taleb Hospital from September 2014 to February 2015. Of these, 687 isolates identified as E. coli were tested for antibiotic susceptibility against 15 antibiotics by the Kirby- Bauer method based on CLSI 2015. The relevant prevalence, percentage, and mean were reported using SPSS (version 16).

A total of 10824 samples were collected. A total of 866 isolates were grown on an agar medium, and 80.3% of the samples were isolated from women. The highest rate of antibiotic resistance was reported to be for Amoxicillin (82.2%). The lowestrate of antibiotic resistance was reported against Nitrofurantoin (14%).

We concluded there is an increasing rate of antibiotic resistance among E. coli isolates. Therefore, the necessity of identifying drug resistance is apparent using precise and straightforward methods to prevent the extensive distribution of antibiotic resistantagents.