فهرست مطالب maryam tahmasebi birgani

Cytogenetics and association studies showed that folate gene polymorphisms can increase the risk of chromosomal nondisjunction and aneuploidies. The folate-metabolizing gene polymorphisms in Down syndrome mothers (DSM) have been assessed in a variety of populations. Reduced folate carrier 1 (RFC1) and cystathionine beta-synthase (CBS) are key enzymes in folate metabolism.

2 common polymorphisms, CBS 844ins68 and RFC1 A80G, were analyzed to determine their probable risk for having Down syndrome (DS) babies in young mothers of Khuzestan province, Iran.

This study was conducted on 100 mothers who had trisomy 21 DS children. 100 age- and ethnic-matched mothers with at least 2 healthy children and no history of abnormal pregnancies were considered as control. The samples were collected from all the mothers from June 2019 to April 2021. Genomic DNA was extracted from peripheral blood. The CBS-844ins68 and RFC1-A80G were genotyped using polymerase chain reaction-electrophoresis and restriction fragment length polymorphism, respectively.

The frequency of RFC1 AG and GG genotypes in DSM was significantly higher than the control mothers (odds ratio [OR] of 2.38 and 3.07, respectively). The heterozygote genotype of CBS 844ins68 was significantly more prevalent among DSM than the control (OR: 2.419). The OR was significantly increased to 6.667 when the homozygote of both variants was found together.

Studying polymorphisms possibly increases the susceptibility of having a DS child. However, ethnicity, nutrition, and epistatic interactions are considerable factors to be evaluated in future studies.

Down syndrome (DS) is a complex genetic disease that is caused by having three copies of chromosome 21. A possible association between polymorphisms in maternal folate metabolism genes and DS has been evaluated.

It was aimed to first investigate the influence of C677T and A1298C polymorphisms in the methylenetetrahydrofolate reductase gene (MTHFR) and plasma homocysteine (Hcy) on the maternal risk for DS in the southwest of Iran.

The MTHFR C677T and A1298C polymorphisms were genotyped using restriction fragment length polymorphism and Sanger sequencing, respectively. Allele and genotype frequencies and the dominant model of the MTHFR C677T and A1298C polymorphisms were evaluated in 80 mothers of children with DS and 80 control mothers. Eventually, the ELISA test was used to compare the concentration of plasma Hcy in both groups.

A significant association was observed in the 677T and 1298C alleles between the mothers of DS and control groups (P = 0.00077 and P = 0.01248, respectively). Further, the median concentrations of Hcy were significantly higher in mothers with DS babies compared to the control group (P < 0.05).

There was an association between MTHFR C677T, A1298C, and plasma Hcy concentrations as the maternal risk of mothers with DS children.

Breast cancer is one of the most common malignancies in women. Treatment with herbal drugs has been considered by researchers due to the lower side effects. Curcumin is a polyphenol extracted from turmeric with confirmed anti-cancer properties. Curcumin is water-insoluble with rapid metabolism. Drug delivery using nano-carriers is suggested to overcome such limitations. The aim of this study was based on evaluating the effect of Dendrosomes curcumin on expression level of HOTAIR long noncoding RNA in MCF-7 breast cancer cell line.

This study was an applied basic research. Firstly, curcumin was loaded in dendrosomes and the entry of dendrosomal curcumin into cells was studied using fluorescent microscopy.  Cell death was investigated using MTT assay and apoptosis detection kit. The expression of HOTAIR gene was measured using real-time PCR. Increased expression of this gene was reported in a wide range of tumors. The data were analyzed using GraphPad Prism V9.5 statistical software, using NOVA one-way statistical analysis and Student t-test; the results were reported as mean ± standard deviation.

Dendrosomes increased the solubility of curcumin. The effective inhibitory doses of dendrosomal curcumin after 24 and 48h treatment were 25 and 20 micromolar, respectively. The percent of cells undergoing early apoptosis were 22.97±0.03 and 56.22±0.05, respectively, which was statistically significant in comparison with non-treated control cells. Following 24 and 48h treatment with 20 micromolar of dendrosomal curcumin, the HOTAIR gene expression was significantly decreased (P=0.001).

These findings suggest that dendrosomal curcumin may promote breast tumor cells toward programmed cell death by reducing HOTAIR gene expression.

The crucial and vital player in tumor recurrence is the tumor-initiating cells (TICs). OCT4 is a widely appreciated non-cell surface for TICs, dedicating detrimental properties to these cells, including self-renewal, epithelial-mesenchymal capacity, and drug resistance. OCT4 and its partners Sox2 and Nanog are up-regulated in stem cells; on the other hand, normal stem cells are more resistant to various herbal remedies like curcumin. Based on these facts, the main objective of the present study was to investigate the alteration of the mentioned genes expression after curcumin treatment in breast cancer cell, human bone marrow mesenchymal stem cells (hBM-MSCs), and non-tumor fibroblast cells (HSFPI3).

MTT assay and AnnexinV/PI were performed to calculate the effective concentration of curcumin. To assess the expression level of OCT4 and Nanog, real-time PCR was performed to quantify the alteration of the mRNA expression of the mentioned genes after treatment in MDA-MB231, hBM-MSCs, and HSFPI3.

Curcumin could not induce significant apoptosis in hBM-MSCs and HSFPI3 even after 24 and 36 hours after treatment in a toxic concentration for cancer cells. After 36-hour treatment with DNC, the mRNA expression of Oct4-B1 in both normal cells enhanced significantly compared to untreated samples. Furthermore, in HSFPI3 cells, the Nanog mRNA expression increased after this treatment.  The expression of both genes decreased in the MDA-MB231 after treatment with DNC.

Non-tumor cells are more resistant to the curcumin treatment compared to cancer cells. The reason is at least partially due to the different expression pattern results in these cells after treatment with this reagent. Pluripotent markers, including Oct4-B1 and Nanog are proposed to play a vital role in these non-tumor cells resistant to curcumin.

 Gastric cancer is one of the most prevalent human malignancy-related death worldwide, which is usually diagnosed at the advanced stages resulting in metastasis. Recent studies have revealed that long non-coding RNAs (lncRNAs), which are known as non-coding RNAs, play a significant role in creating variety of molecular pathways (e.g., growth, proliferation, differentiation, and apoptosis) and negatively contribute to many unusual processes, including human cancers. The HOX antisense intergenic RNA (HOTAIR) is one of the novel non-coding RNAs which has recently emerged as a promoter of metastasis in different types of human cancers through epithelial-to-mesenchymal transition (EMT) process. Epithelial-to-mesenchymal transition (EMT) is a cellular process where an epithelial cell could change its phenotype to mesenchymal condition, which plays a crucial role in promoting cell invasion, angiogenesis, and metastases.

 This study aimed to explore the effect of HOTAIR gene silencing on the expression levels of two main markers of EMT signaling pathway, fibronectin (FN1) and claudin4 (CLDN4) in MKN45 cellular model of gastric cancer. The MKN45 cells were subjected to HOTAIR specific siRNA for 48 hours, and the extracted RNAs were subjected to cDNA synthesis and real-time PCR. The expression change was calculated using 2-ΔΔct.

 Our findings showed that FN1 and CLDN4 were upregulated in MKN45 cells. Following the transfection of cell by HOTAIR siRNA, both FN1 and CLDN4 genes were significantly downregulated.

 HOTAIR long non-coding RNA may have regulated the expression levels of FN1 and CLDN4 genes in EMT signaling pathway. However, it was recommended that further experimental analyses should be carried out to confirm this observation. In other word, our study result may not have been applied as a therapeutic access until additional experiments were conducted.

The COVID-19 pandemic is a red alarm for global health, so researchers around the world are working on it to design an effective vaccine against it. Protein is one of the candidates for vaccine development which plays an important role in virus pathogenesis. Accordingly, this study was done to evaluate the critical characteristic of this protein as a vaccine candidate using in-silico analysis. The sequence of SARS-CoV-2-associated E protein was recruited from NCBI and subjected to the IEDB software to evaluate the most potent epitopes. The capacity of the interactions of HLA-I and HLA-II molecules with selective peptides was studied using IEBD tool kit. The E protein sequence was subjected to B cell and T cell tests to realize the most promising peptides that could act as COVID-19 vaccine. Among the tested peptides for the T cell-test, this study found two interesting epitopes: VSEETGTLI and LTALRLCAY that exhibit high binding affinity as a strong indicator to HLA-I and HLA-II alleles together. The results of the analysis demonstrated that some epitopes in the E protein have a relatively higher immunogenicity score based on interaction with HLA-II, such as SEETGTLIVNSVLLF, TLIVNSVLLFLAFVV, LAFVVFLLVTLAILT, LAILTALRLCAYCCN, and SVLLFLAFVVFLLVT. Furthermore, two sequences (FVSEET and PSFYVYSRVKNLNSSRVP) were reported as the selective linear epitopes for B cell, on the surface of SARS-CoV-2 E protein and being Immunogenic. Since E protein can stimulate favorable immune responses, T and B- cell responses, its evaluation in patients with COVID-19 is of a great importance.

Background: The objective of this study was to design and construct a CO2 incubator with nonmetallic walls and to investigate the viability of the cells and microwave irradiance inside this incubator. Methods: Because the walls of conventional incubators are made of metal, this causes scattering, reflection, and absorption of electromagnetic waves. We decided to build a nonmetallic wall incubator to examine cells under microwave radiation. Incubator walls were made using polyvinyl chloride and Plexiglas and then temperature, CO2 pressure, and humidity sensors were placed in it. Atmel® ATmega1284, a low‑power CMOS 8‑bit microcontroller, collects and analyzes the sensor information, and if the values are less or more than the specified limits, the command to cut off or connect the electric current to the heater or CO2 solenoid valve is sent. Using a fan inside the incubator chamber, temperature and CO2 are uniforms. The temperature of the points where the cell culture plates are placed was measured, and the temperature difference was compared. Ovarian cancer cells (A2780) were cultured in the hand‑made and commercial incubators at different times, and cell viability was compared by the MTT method. Microwave radiation in the incubator was also investigated using a spectrum analyzer. The survival of cells after microwave irradiation in the incubator was measured and compared with control cells. Results: The data showed that there was no significant difference in temperature of different points in hand‑made incubator and also there was no significant difference between the viability of cells cultured in the hand‑made and commercial incubators. The survival of irradiated cells in the incubator was reduced compared to control cells, but this reduction was not significant. Conclusion: This incubator has the ability to maintain cells and study the effects of electromagnetic radiations on the desired cells, which becomes possible by using this device

MicroRNA-155 (miR-155) has a critical role in pro-inflammatory activation and tumor progression. In addition, miR-155 has various oncogenic effects in the tumor microenvironment by targeting the suppressor gene of cytokine signaling-1(SOCS-1) and interleukin-6 (IL-6). This study investigated the association of inflammatory changes with the variations of miR-155 expression in newly diagnosed breast cancer (NDBC) patients. Seventy NDBC patients were categorized as lobular and ductal subgroups and forty healthy individuals participated in this study. The expression rate of miR-155 and its downstream target gene, SOCS-1, as well as the plasma levels of IL-6, were evaluated in peripheral blood mononuclear cells of NDBC patients; using real-time PCR and enzyme-linked immunosorbent assay, respectively. Our results indicated an over-expression of miR-155 in the PBMCs of NDBC patients which was significantly associated with the tumor grade and the type of ductal carcinoma. In contrast, a significant downregulation of SOCS-1 was observed in NDBC patients compared to control group, however, there was no significant difference between two subtypes of BC. Furthermore, a higher concentration of plasma IL-6 was detected in NDBC patients compared to the healthy control group which had an inverse correlation with the SOCS-1 levels. According to the potential effects of miR-155 on regulating the expression of SOCS-1 and IL-6, we suggest this small transcript as a promising diagnostic marker for various types of BC patients.

HOX transcript antisense RNA (HOTAIR), as a long noncoding RNA (lncRNA) is a highly cited transcript modulating variety of signaling pathways such as cell growth and apoptosis. Altered expression of HOTAIR has been reported in human cancers, which contributes with cancer progression and metastasis. Increased expression level of HOTAIR has been observed in colorectal cancer (CRC). It seems that dysregulation of HOTAIR may inhibit the apoptosis. The present study was aimed to evaluate the effect of HOTAIR silencing on expression of apoptosis markers Bax and Bcl2 using real-time polymerase chain reaction (PCR). The data showed that HOTAIR and Bcl2 are highly expressed in CRC cells while the expression level of Bax is low. Following siRNA treatment, Blc2 was downregulated but Bcl2 was upregulated. These findings suggest that HOTAIR silencing can promote apoptosis, and thus it can be considered as a promising strategy to kill cancer cells.

 Gastric cancer is the second reason for cancer mortality worldwide, with a high capacity for metastasis. Long non-coding RNAs (lncRNAs) are recently described as lengthy transcripts with no open reading frame. The lncRNAs play an important role in critical cellular and molecular pathways, including cell cycle, growth, differentiation, and apoptosis. Therefore, it is not surprising that abnormal expression of lncRNAs may be involved in human cancers. The HOX antisense intergenic RNA (HOTAIR) is a highly cited lncRNAs whose altered expression has been reported in a variety of human cancers such as gastric cancer. Epithelial to mesenchymal transition (EMT) is a cellular route in which an epithelial phenotype of the cells can be changed into the mesenchymal state. The signaling pathways involved in EMT are related to cancer metastasis and recurrence of gastric cancer.

 The present study was aimed to investigate the effect of HOTAIR gene silencing on expression levels of fibronectin 1 (FN1) and claudin-4 (CLDN4) genes, two important markers of EMT, in AGS cellular model of gastric cancer. The AGS cells were exposed to the HOTAIR-specific siRNA for 48 hours. The extracted RNAs were subjected to complementary DNA synthesis and real-time PCR. Data were analyzed using 2−ΔΔCt method. Cells with no siRNA treatment were considered the control set. The P-value < 0.05 was considered statistically significant.

 The observed data showed that the expression levels of two EMT markers FN1 and CLDN4, were significantly decreased after HOTAIR silencing.

 This study demonstrates that HOTAIR can regulate the EMT signaling pathway through critical EMT factors like FN1 and CLDN4 transcripts. However, a long way remains to apply this finding in therapeutic approach, and further experiments are needed.

Hyperlipidemia is one of the main risk factors for coronary artery disease and is defined as abnormal elevation of lipids or lipoproteins in the blood. Pcsk9 is the ninth member of the proprotein convertase family that binds to the LDLR on the surface of the hepatocyte, leading to degradation of LDLR in lysosomes which could cause hyperlipidemia. The present study aimed to analyze the methylation status of pcsk9 promoter in patients with hyperlipidemia.

This case-control study was conducted in 50 patients with hyperlipidemia and 50 healthy controls. DNA isolation from whole blood was performed using salting out procedure. Promoter methylation of the Pcsk9 gene was analyzed by methylation-specific PCR (MSP). Ten MSP products were sequenced to confirm the data obtained.

According to MSP results, methylation pattern of pcsk9 gene promoter displayed an unmethylated status among the patients and control individuals. In other words, no methylation was seen in case and control samples.

The current study showed no significant association between PCSK9 methylation pattern and blood lipid profile level in case group and control group.

Helicobacter pylori is one of the most prevalent human infectious agents that is directly involved in various upper digestive tract diseases. Although antibiotics-based therapy and proton pump inhibitors eradicate the bacteria mostly, their effectiveness has been declined recently due to emergence of antibiotic-resistant strains. Development of a DNA vaccine is a promising approach against bacterial pathogens. Genes encoding motility factors are promising immunogens to develop a DNA vaccine against H. pylori infection due to critical role of these genes in bacterial attachment and colonization within the gastric lumen. The present study aimed to synthesize a DNA vaccine construct based on the Flagellin A gene (flaA), the predominant flagellin subunit in H. pylori flagella.

The coding sequence of flaA was amplified through PCR and sub-cloned in the pBudCE4.1 vector. The recombinant vector was introduced into the human dermal fibroblast cells, and its potency to express the flaA protein was analyzed using SDS-PAGE. The recombinant construct was intramuscularly (IM) injected into the mice, and the profiles of cytokines and immunoglobulins were measured using ELISA.

It has been found that flaA was successfully expressed in cells. Recombinant-vector also increased the serum levels of evaluated cytokines and immunoglobulins in mice.

These findings showed that the pBudCE4.1-flaA construct was able to activate the immune responses. This study is the first step towards synthesis of recombinant-construct based on the flaA gene. Immunization with such construct may inhibit the H. pylori-associated infection; however, further experiments are urgent.

Colorectal cancer as a leading cause of cancer related death develops through multistep pathway. MicroRNAs (microRNAs) are a group of small noncoding RNAs that inhibit target mRNA translation. Studies show that abnormal expression of these genes is associated with colorectal cancer. Studies have shown that abnormal expression of these genes is associated with colorectal cancer. miR-195 appears to be the major regulatory factor in various cancers including colorectal cancer. Further studies are needed to precisely determine the regulatory role of miR-195 in colorectal cancer. The aim of this study was to determine the expression level of miR-195 in colon adenocarcinoma and normal tissue specimens and to confirm this biomarker in tissue samples in Iran.

The study was a case-control design. In vitro bioinformatics analysis was performed on gene expression and its results were confirmed by experimental and in vitro analysis on clinical samples. In this study, after RNA extraction and cDNA sequencing, gene expression was designed using primers. Real time PCR was measured and then statistically analyzed.

The initiation of invasion and metastasis pathways may be due to decreased miR-195 expression. The qRT-PCR test results for the expression of genes showed that mi3R-195 expression was potentially decreased in tumor tissues compared to adjacent normal tissues (p <0.00001). miR-195 is potentially associated with clinicopathologic features of tumors (P value 0.2363).

The present study demonstrates the potential role of 195 - miR in the development of colon cancer.

Long noncoding RNAs (lncRNAs) are lengthy noncoding transcripts which are actively involved in crucial cellular pathways. Tissue-specific expression of lncRNAs beside their secretion into the body fluids, have made lncRNAs in attention as biomarkers of the diseases. According to the role of lncRNAs, especially H19 in cardiac regeneration, it is not surprising if their altered expression levels lead to cardiac diseases. In the present study, the relative expression of H19 was compared in the plasma of atherosclerotic myocardial infarction and control individuals by real time- PCR, and data were normalized using GAPDH. The association of plasma level of lipid and homocystine with H19 expression was also considered. The potential of H19 to discriminate the case from control was studied using the ROC analysis. We found that plasma level of H19 transcript was significantly increased in the plasma of patients in comparison with the control group. Additionally, the relative expression level of H19 was directly associated with the plasma homocystine level. The relative expression of H19 at threshold of 0.3 showed 70% sensitivity and 94% specificity to discriminate cases from controls. This study revealed that the expression level of H19 may be considered as a biomarker of myocardial infarction although further studies are needed to generalize this finding.

Acinetobacter baumannii is one the most dangerous opportunistic pathogens in hospitalized infections. This bacterium is resistant to 90% of commercial antibiotics. Therefore, developing new strategies to cure A. baumannii-infections is urgent. The DNA vaccines new approach in which the immunogen can be directly expressed inside the target cells through cloning of immunogen into an expression vector. The outer membrane protein A(OmpA) is one the critical factors in pathogenicity of A. baumannii which has been repeatedly described as a powerful immunogen to trigger the immune responses. As the pure form of the OmpA is insoluble, vaccine delivery is very hard. We previously cloned the ompA gene from A. baumannii into the eukaryotic expression vector pBudCE4.1 and observed that the OmpA protein has been considerably expressed in eukaryotic cell model. In current study, the immunogenic potential of pBudCE4.1-ompA has been evaluated in mice model of experimental. The serum levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ were measured by enzyme-linked immunosorbent assay (ELISA) after immunization with ompA-vaccine. The protective efficiency of the designed-DNA vaccine was evaluated following intranasal administration of mice with toxic dose of A. baumannii. Obtained data showed the elevated levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ in serum following the vaccine administration and mice who immunized with recombinant vector were survived more than control group. These findings indicate ompA-DNA vaccine is potent to trigger humoral and cellular immunity responses although further experiments are needed.

Charcot-Marie Tooth disease (CMT) is one of the main inherited causes of motor and sensory neuropathies with variable expressivity and age-of onset. Although more than 70 genes have been identified for CMT, more studies are needed to discover other genes involved in CMT. Introduction of whole exome sequencing (WES) to capture all the exons may help to find these genes. Here, we tried to find the genetic cause of the neuropathy in two Iranian brothers using WES. Blood sample was collected from probands and their family members to extract the genomic DNA. The extracted DNA from one of the affected case was subjected for WES. The variant calls were filtered  to reveal the pathogenic variant. Presence of the candidate mutation was confirmed using Sanger sequencing. The pathogenic potential of the variant was examined using in silico software. Using ClustalW multiple alignment, the presence of variant in conserved domain of protein was investigated. The parent and another affected boy were also checked for presence of the variant using PCR-sequencing. The obtained data presented a novel TTC del mutation in CDS 738 of dystrophin related protein 2 (DRP2) gene, which was validated by sequencing. The variant was located in a conserved domain of DRP2 protein and predicted as pathogenic. Two affected boys were hemizygous for the mutation and received the mutation from mother. Here, we provided the evidence for the contribution of DRP2 in CMT. Also, the symptoms shed light on molecular aspect of this genetically heterogeneous disease.

Acinetobacter baumannii is one the most dangerous microbes that is resistant to a wide range of prescribed antibiotics, thus development of more effective approaches is urgent. Recombinant protein vaccines have been recently proposed as a safe method to cope with infectious agents. In this approach, antigenic gene of a bacterium can be cloned in an expression vector to produce recombinant protein. In this study, the prevalence of A. baumannii was determined in hospitalized samples and the antimicrobial susceptibility patterns of isolates were characterized for a variety of antibiotics. The ompA gene from resistant isolates was then amplified and cloned in an expression vector to produce the recombinant OmpA protein in Escherichia coli. The clinical samples were collected from sputum, wounds, septicemia, and urinary tract infections at the intensive care unit (ICU) and different wards of hospitals and A. baumannii were identified, according to standard diagnostic tests. The antibiotic susceptibility patterns of the isolates were determined for 17 antibiotics. The ompA gene of A. baumannii was amplified using the polymerase chain reaction (PCR). The ompA-amplicon was cloned and sub-cloned in pTZ57RT and pET32a (+) vectors, respectively. Double digestion, DNA sequencing, and PCR were performed to confirm that ompA has been truly cloned. Using RT-PCR and SDS-PAGE, the expression of recombinant OmpA was assessed in IPTG-induced E. coli. Most of the A. baumannii were resistant to antibiotics cefepime, ceftazidime, ceftriaxone, aztreonam, amikacin, and gentamycin, and the least resistance was found towards colistin, ampicillin-sulbactam, and trimethoprim antibiotics. The ompA gene was amplified as 1112 bp amplicon, which was successfully cloned and sub-cloned in the pTZ57RT-T/A and pET32a (+) vectors. The presence of ~38 kDa band in SDS-PAGE showed that the recombinant pET32a-ompA was highly expressed in the host cells. The obtained data showed the high resistance of A. baumannii-infected isolates to the antibiotics. Besides, successful cloning and expression of rOmpA in the cells suggests that the antigenic properties of ompA gene may be considered in future researches.

Bardet-Biedl syndrome (BBS) is a pleiotropic and multisystemic disorder characterized by rod-cone dystrophy, polydactyly, learning difficulties, renal abnormalities, obesity and hypogonadism. This disorder is genetically heterogeneous. Until now, a total of nineteen genes have been identified for BBS whose mutations explain more than 80% of diagnosed cases. Recently, the development of next generation sequencing (NGS) technology has accelerated mutation screening of target genes, resulting in lower cost and less time consumption. Here, we screened the most common BBS genes
(BBS1-BBS13) using NGS in an Iranian family of a proposita displaying symptoms of BBS. Among the 18 mutations identified in the proposita, one (BBS12 c.56T>G and BBS12 c.1156C>T) was novel. This compound heterozygosity was confirmed by Sanger sequencing in the proposita and her parents. Although our data were presented as a case
report, however, we suggest a new probable genetic mechanism other than the conventional autosomal recessive inheritance of BBS. Additionally, given that in some Iranian provinces, like Khuzestan, consanguineous marriages are common, designing mutational panels for genetic diseases is strongly recommended, especially for those with an autosomal recessive inheritance pattern.

Curcumin is a hydrophobic polyphenol extracted from the plant curcuma longa with established anticancer properties. However, curcumin benefits have been impaired by its very low water solubility, low absorption, rapid metabolism and clearance from the body. Recently, nanotechnology promises to be helpful in development of drugs delivery systems by recent advances in macromolecular design of nanocarriers. In this review, we present the novel generation of nano-vehicles termed dendrosomes which are readily synthesized from esterification of oleic acid and polyethylene glycol residues. Dendrosomes efficiently encapsulate curcumin in a spherical micellar or polymersome structures which leads to increase aqueous solubility of this hydrophobic agent and higher bioavailability of curcumin. Anticancer potency of this nanoformulation was confirmed in different mouse and human cancer cells including fibrosarcoma, colon, glioblastoma, bladder, gastric, breast and hepatocellular carcinoma in vitro and vivo. It has also demonstrated that this nano preparation has no cytotoxicity effects on normal cells. Finally, these results introduce dendrosomal curcumin as potent anti-tumor agent although further clinical examinations are needed.