فهرست مطالب marzieh derakhshan-horeh

Transient global cerebral ischemia (TGCI) is induced by occlusion of the bilateral common carotid artery occlusion (BCCAO), and it mediates neuronal cell death in the CA1 hippocampal area. AM251 is a cannabinoid receptor type 1 (CB1) blocker that has been known to be protective against transient focal cerebral ischemia. JWH-015 is a selective agonist of CB2 and activator of CB1 that is involved in the promotion of neuronal recovery and survival. The aim of this study was to investigate the role of combined application of JWH-015 and AM251 in the rat model of GCI.
Male Wistar rats underwent 20 minutes of ischemia followed by reperfusion. Then, 1 mg/kg JWH-015 and 2 mg/kg AM251 were administered through the caudal vein. The groups were control, sham, ischemia, vehicle, AM251, JWH-015 and AM251 JWH-015. Animals were sacrificed 1 week after BCCAO.
The AM251 JWH-015 group indicated a significant increase in the protein expressions of AKT1, Bcl-XL, Bcl-2 and Bad 14-3-3, but it showed a considerable decrease in the protein expressions of Bad and JNK1/2 (P ≤ 0.05 vs. AM251, and JWH-015 groups). The AM251 JWH-015 group had a significantly higher number of alive cells and lower number of apoptotic cells in the CA1 hippocampal area and it also had a considerable improvement in spatial memory (P ≤ 0.05 vs. AM251, and JWH-015 groups).
The results of this study indicated that combined application of AM251 and JWH-015 could be neuroprotective against detrimental effects of ischemia probably via suppression of neuronal apoptosis and maintenance of their survival.

A reasonable number of male infertility cases are related to genetic factors. Considering the high prevalence of chromosomal abnormalities related to male infertility, this study investigated the association of the chromosomal aberrations and chromosome variants with hormonal levels, a positive family history, parental consanguinity and a specific lifestyle. We also aimed to find a predictive factor to estimate the risk of the presence of an abnormal karyotype in the azoospermic and especially sever oligozoospermic men.
A total of 230 infertile men and 50 healthy controls enrolled in the study for cytogenetic evaluation. Data on patients" characteristics were gathered, accurately.
Among aforementioned factors, only luteinizing hormone (LH) >12 IU/l raised the chance of detecting a chromosomal abnormality (P Conclusiond: This study suggests a positive family history of infertility, parental consanguineous marriages and high levels of FSH as strong determinants or risk factors for male infertility. Nonetheless, the presence of these patient characteristics did not prove to have a direct correlation with chromosomal abnormalities in male infertility. Among the various possible risk factors studied, an elevated gonadotropin level provides a better risk assessment for the incidence of chromosomal abnormality in infertile men.