marzieh tadi

Rosa damascena Mill is from Rosacea family which has been used in traditional medicine for the treatment of gastrointestinal diseases. The effects of petal’s hydro-alcoholic extract of Rosa damascena Mill were examined on the trachea, aorta and uterus of Wistar rats in this study. This experimental study was conducted on forty male Wistar rats randomly divided into five groups of eight including control group, the groups receiving 0.5, 0.75 and %1 doses of Rosa damascena Mill extract with the presence and absence of calcium chloride. Isometric contraction was recorded by adding KCl to smooth muscle with stability device. After reaching the pan state, the effects of saline and cumulative concentrations of extracts were recorded and the percentage changes were calculated. Cumulative extract of Rosa damascena Mill (0.5, 0.75 and 1μg/m) dependent on dose caused the rat’s trachea, aorta and uterus contractions (p<0.05). The contractions of cumulative extract of Rosa damascena Mill were 54± 2/37, 96±3/32 and 62±2/17 in aortic smooth muscle, trachea and uterus, respectively with the presence of 60mM calcium chloride. The results of this study showed that Rosa damascena Mill increased the trachea, aorta and uterus contractions. Therefore it should be used with caution in patients suffering from asthma, dysmenorrhea or hypertension.

Pathogens can be transmitted to the humans through the consumption of contaminated meat and thus causing disease. Shiga-toxin-producing Escherichia coli can cause mild watery diarrhea to more serious complications of hemorrhagic colitis، and hemolytic uremic syndrome to even death. Present study was conducted to detect Shiga toxin-producing Escherichia coli from sheep meat (lamb) in ChaharmahalvaBakhtiari، Iran. 90 Escherichia coli isolates from sheep meat in ChaharmahalvaBakhtiari were evaluated to investigate stx1، stx2 and eaeA genes by multiplex PCR. The rate of stx1 and eaeA-positive Escherichia coli isolates were 11. 11% (10/90) and 8. 88% (8/90)، respectively. Stx2 gene was not found in any isolate. Discussion and Lamb harbored Shiga-toxin-producing E. coli and could be a component of Shiga-toxin-producing E. coli transmission from lambs to the humans and can pose a risk to human health in the region.