فهرست مطالب masoud imani

The aim of this study was to investigate the effects of long-term administration of clenbuterol on the histomorphometry and histopathology of testicular tissue in mice. 30 male NMRI mice were divided into three groups including the control group that received (0.1 ml of normal saline) the second group, (2.5 mg /kg clenbuterol) and the third group (8 mg/kg clenbuterol) that received for 30 consecutive days. At the days 3 and 30 from the beginning of the study, five mice were randomly sacrificed from each experimental group. The testicular tissue was removed from the right testis and fixed in the Bouin's solution for histomorphometric and pathological examinations. Degenerative lesions including distortion of the germ cells, reducing their number, and presence of vacuoles within the seminiferous epithelium were demonstrated in the clenbuterol treated groups. At the present study, administration of clenbuterol decreased spermatogenesis parameters including meiosis index, Johansen score, spermatogenesis percentage, seminiferous tubules diameter and epithelium height of seminiferous tubules both in the day 3 in low and high dose of clenbuterol with more severity in high dose. On the 30th day, testicular degenerative changes due to clenbuterol were more in the low dose of rather than high dose. According to the obtained results, it can be concluded that administration of clenbuterol may has potentially negative affect on the sperm production and fertility in male mice.

Equine Viral Arteritis (EVA) is a transmissible disease in the horse population, which caused by RNA viruses of family Arteriviridae. Abortion in mares, lethal pneumonia and enteritis in young foals may occur in infections. The aim of this study was to detection of Equine Arteritis Virus antibodies in horse population of Kerman city and Bojnurd city of Iran for the first time. sera were collected from apparently healthy horses randomly from the horse population of Kerman city and Bojnurd city in the period from Sep to Feb . The samples were examined by indirect ELISA test with commercial kit. The results indicate that the seroprevalence of EAV infection in the region is . EVA seropositivity was not associated with age, sex, breed and keeping system. According to the present study, EVA existed in the horse populated areas of Iran and Therefore, implementation of control programs to prevent EVA outbreaks seems necessary.

Reproductive toxicity of cadmium (Cd) as an environmental toxicant has been proved in animals and humans. Exposure to Cd impairs testes organs and can reduce male fertility. The present study was designed to investigate the spectrum of histopathological changes in testicular tissue focusing on Sertoli cells in rats following Cd intoxication.

In the present experiment, acute testicular toxicity was induced by an intraperitoneal injection of 1.2 mg/kg CdCl2 to the animals in the test group, while the control group received normal saline. After 52 days, the animals were euthanized, and testicular tissue was stained by Hematoxylin and Eosin. In addition, immunohistochemical staining was performed on Sertoli cells for Wilms' Tumor, Melan-A, and CD99 to evaluate histopathological changes.

Cd caused significant alterations in seminiferous tubules with varying effects on the patterns of spermatozoa production. These histopathological changes were significantly higher in the Cd group, compared to the control group.

The Cd-induced stepwise spectrum changes included sloughing, disorganization, hypospermatogenesis, spermatic cell arrest, germ cell hypoplasia, Sertoli cell-only pattern, fibro-hyalinized seminiferous tubules, and calcification. Sertoli cells accumulated and created multinucleated giant cells in the seminiferous tubules during the atrophic process, which could be dependent upon Sertoli cells viability and function.

Methenamine is a worldwide antibacterial agent for urinary system infections in human and animals. The effect of methenamine consumption during early phase of pregnancy is not fully clarified in previous studies. Vascular development is the essential part of the early embryonic growth.

In this study, we used chicken chorioallantoic membrane to evaluate the effects of methenamine administration on angiogenesis process as a model.

in this experimental study, 20 Ross 308 eggs (mean weight 55±4) were incubated. The eggs were divided into two equal groups (n = 10/each). In the first group, methenamine (150 mg/kg egg weight) was injected on the shell membrane, and in the second group (control group) phosphate-buffered salineas injected. Methenamine was inoculated at 96 and 120 hrafter incubation; 24 hrafter the last inoculation, the eggs were removed and the egg’s shell was incised. Then, the development of vascular network and vascular endothelial growth factor Aexpression was evaluated.

Angiogenesis was significantly decreased after methenamine treatment. The indexes such as areas containing vessels, the vessels’ length, the percentage of angiogenesis developing areas, and vascular complexity in the treatment group receiving methenamine were significantly reduced compared to the control group. Vascular endothelial growth factor Aexpression was suppressed in the methenamine treated group.

According to the achieved results, it was defined that methenamine could have an inhibitory effect on the growth and development procedures of extraembryonic vasculature.

The aim of this study was to evaluate the effect of eCG, PGF2α, and combination of eCG and PGF2α early postpartum on reproductive performance in high producing dairy cows. Three hundred sixty eight postpartal Holstein dairy cows were divided in 4 groups. Cows in groups 1 and 2 received 500 IU eCG on day 8 ± 2 and cows in groups 3 and 4 received saline. Cows in groups 1 and 3 received injections of 500 µg cloprostenol twice 8 hours apart between days 20 to 25 postpartum, and cows in group 2 and 4 received saline. Presentation of a functional CL was assessed by ultrasonography of ovaries and serum progesterone concentration in groups 1 and 3. None of the treatments could improve fertility, and reproductive indices including 120 days in milk pregnancy rate, pregnancy loss, first service pregnancy rate and calving to conception interval was not different among the various different groups. Only days to first service in eCG treated cows was marginally lower than that of control cows. Treatment with eCG in cows in group 2 (eCG놩抝) had no effect on the initiation of ovarian cyclical activity by day 30 ± 1 postpartum. Resumption of ovarian cyclical activity by day 30 ±1 postpartum did not affect reproductive performance in dairy cows. Additionally, treatment with eCG, PGF2α and combination of eCG and PGF2α had no effect on the prevalence of clinical endometritis, anovulatory anestrus, and follicular cyst. In conclusion, early treatment of high producing dairy cows after parturition with eCG and PGF2α had no effect on reproductive performance of lactating dairy cows.

Case Description: Hirudiniasis in the subglossal area is very rare. We describe an Iraniancross-breed goat:kid with two leeches’ infestation in his sublingual area.Clinical The animal was referred to the veterinary clinic with a history of anorexia, teeth grinding and bleeding from the mouth. In clinical examination of the mouth, two leeches observed which attached to sublingual region.Treatment and Outcome: Manual removal by forceps was done and all clinical signs were subsided.Clinical Relevance:Most leeches are bloodsucking parasites which attach themselves to vertebrate hosts, bite through the skin and suck out a quantity of blood. Other than the nuisance effect of their biting, their medical significance is generally minimal