فهرست مطالب masumeh heidari

 The quality of work life is a sign of an organizational culture based on which employees feel self-sufficiency and self-esteem. Considering that such a study has not been conducted among the operating room staff, the present study aimed to determine the quality of work life among the operating room staff of selected hospitals in Tehran in 2021.

 The current research is a descriptive-cross-sectional study. Sampling was done as a cluster and the samples were selected from the operating room staff of selected government hospitals in Tehran in 2021. In total, 231 participants answered questions related to demographic information and Walton's quality of work life questionnaire (35 options) in the form of self-report. The data were analyzed with the help of descriptive and inferential statistical methods in SPSS-24 software at a significance level of 0.05.

 The participants age averaged (standard deviation) at 31.69 (6.35). The mean (standard deviation) of the quality of work life of operating room workers was 96.04 (18.28), so that 23.8% had low levels of work life quality, 73.2% had average and 3% had high work life quality. The overall work life environment and safe and healthy work environment revealed the lowest and highest scores, respectively.

 It seems that the quality of working life of the operating room staff is not at the optimal level. Therefore, the treatment officials should provide the necessary platforms to improve the quality of work of the operating room staff in different areas, so that the staff can serve with more motivation.

In recent years, there have been changes in the attitude of girls towards marriage and it has affected their desire to get married. The present research was conducted with the aim of comparing the attitude towards marriage of the female students of Shahid University before and after holding the family knowledge course in the first half of 2019-2019.

The method of descriptive-analytical research was before and after comparison. The research was conducted on 159 undergraduate female students of Shahid University in Tehran. Sampling was done as available, and the samples were students who had taken a two-unit course on population and family knowledge in the second half of 1399-1400. Data was collected using the Marriage Attitude Scale (MAS) with four subscales of optimistic, pessimistic, realistic and idealistic views. The questionnaire was completed by the students once before the beginning of the course and the second time three months later (after the completion of 14 teaching sessions of population and family knowledge). Data analysis was done with spss software version 21.

The average age of the subjects studied was 21.19 ± 3.88 years. The difference of general attitude towards marriage before 77.87 ± 9.48 and after 83.57 ± 9.25 was significant (p<0.001). The sub-scales of attitude towards marriage, including optimistic, idealistic and realistic attitude towards marriage, before and after, had a significant difference (p<0.001).   But the pessimistic attitude had no significant difference (p=0.615).

According to the results of the research, the population and family knowledge course has been able to improve the general attitude towards marriage, and considering the decrease in the marriage rate in recent years, it is better to present this course in the first year of education to motivate marriage will be established in them sooner.

Sexual functioning during pregnancy presents couples with many challenges. The purpose of this study was to determine the effect of an educational intervention based on PLISSIT model on sexual satisfaction of pregnant women in the third trimester of pregnancy.

This was a non-randomized clinical trial. A sample of pregnant nulliparous women who referred to a large general hospital in Tehran was entered into the study. The sexual satisfaction questionnaire (MWSSQ) and a demographic questionnaire were used to collect data. The Data were analyzed by SPSS software using descriptive statistics, independent t-test and chi-square and repeated measures analysis.

While there was no significant difference in the mean sexual satisfaction of pregnant women before intervention (p = 0.91), 4 weeks after training, the sexual satisfaction of pregnant women in the intervention group was significantly higher than the control group (p = 0.027).  However, at the end of the second trimester and the end of the third trimester sexual satisfaction were higher in the intervention group as compared to the control group, but did not show any significant difference

Sexual education during pregnancy seems improve sexual satisfaction in women. Indeed it is suggested that sexual education be included in routine prenatal care programs.

The aim of this study was to evaluate the impact of husband's participation in sexual education on sexual function during pregnancy.
This quasi experimental study was conducted on 123 couples who were divided in two intervention (A: couples, B: pregnant women) and one control (C) groups. Group A couples received sex education, Group B women received sex education without their spouses, and Group C women received routine prenatal care without sex education. Sexual functions of couples were assessed by Female Sexual Function Index (FSFI) and International Index Erectile Function (IIEF) questionnaires, before sex education, four weeks after the intervention, at the end of the second trimester and at the end of the third trimester.
Mean total scores of FSFI and IIEF were not different at baseline in three groups. Repeated measure analysis showed significant differences between groups (A and B with C) in the mean total scores of FSFI and IIEF during the third trimester. The mean total scores of the two intervention groups of A and B were not significant.
According to the results of the present study, promoting the sexual function of pregnant women needs to include the sex education on prenatal care. Whereas spouses’ participation was suggested to have a great role in the effectiveness and strengthening of the education in various studies, this study showed that the lack of spouses’ participation for whatever reasons may lead to the same results of previous studies which emphasized the necessity of spouses’ participation.

Aim: The aim of this study was to evaluate the impact of the PLISSIT model-based counseling on the sexual function of couples during pregnancy.
This quasi-experimental study was carried out in Tehran (Najmieh Hospital) on 88 couples who were randomly assigned to the control (44 couples) and intervention (44 couples) groups. The intervention group received sexual counseling based on a PLISSIT model by a trained midwife, and the control group received only routine services in prenatal care. Sexual functions of the couples were assessed by the Female Sexual Function Index (FSFI) and International Index Erectile Function (IIEF) questionnaires before counseling, 4 weeks after counseling, and at the end of the second trimester of pregnancy. Independent t-test and repeated measures analysis of variance (ANOVA) test were used for comparison of the scores within the groups.
Findings: No significant differences were found in the demographic variables of the subjects. The mean total scores of FSFI and IIEF were not different at baseline in the two groups. Repeated measure analysis showed significant differences between the two groups in the mean total scores of FSFI and IIEF after the intervention. Post intervention, the mean of the total score of sexual function of the couples was significantly higher in the intervention group compared with the control group (P Based on the results of this study, sex education for prenatal care would be effective, and we can claim that sexual function of couples during pregnancy may be enhanced by using the PLISSIT model.

Epidermal growth factor receptor (EGFR) plays a major role in the pathophysiology of a wide variety of solid tumors such as glioblastoma and breast cancer. Therefore, blocking of signaling cascade of this receptor via specific antibodies is an appropriate therapeutic target against these cancers. The first step to make monoclonal antibodies is production of recombinant protein with high purity and glycosylation pattern similar to human protein. One of the best available hosts for this purpose is the Pichia pastoris yeast. Coding sequence of extracellular and transmembrane domain of EGFR protein was isolated from human glioma cell line (A172) using real-time polymerase chain reaction (RT-PCR) technique. This sequence was cloned into plasmid pPicZαA and transferred into Pichia pastoris yeast cells. Then, the production of recombinant protein was induced via treating of cells with methanol with final concentration of 0.5%, in several time periods, 24, 48, 72, 96, 120, 144, 168 and 192 hours. Protein production was assessed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Partial coding sequence of EGFR was cloned in pICZαA plasmid. The results of induction of protein expression on SDS-PAGE gel showed that the protein expression increased as incubation time increased. However, after three days of induction, the secreted host proteins in the culture medium increased and got visible on SDS-PAGE gel. In this study, we produced EGFR protein that can dramatically speed up production process of EGFR inhibiting monoclonal antibodies.