فهرست مطالب mehdi pooladi

Breast cancer is one of the most common cancers in the world. Treatment methods for this disease have been with poor results. In recent years, more attention has been paid to stem cells due to their cytotoxic properties. According to previous results, the effect of nanoquercetin on breast cancer cells in this study was used concomitantly with doxorubicin, which is a chemotherapy drug and in some patients has therapeutic resistance.

The above study was performed on MCF7 and BT474 human breast cancer cells. Quercetin was synthesized, then different concentrations of 10, 20, 40, 80 and 160 of quercetin and 1, 2.5, 5 and 10 μg/ml of doxorubicin were prepared and two cell lines were treated for 24 h. MTT test was used to determine cell viability and flow cytometry test was used to determine the extent of apoptosis in both cell lines.

The results showed that MCF7 and BT474 cells that were affected by quercetin and doxorubicin, increasing the concentration of quercetin and doxorubicin decreased the survival of both cell lines within 24 h. The LC50 concentration was calculated for both cells treated with nanoquercetin 40 μg/ml and for doxorubicin doxorubicin (0.1 mg/ml). The results also showed that in both cell lines, an increase in cellular apoptosis occurred after incubation with quercetin and then incubation with doxorubicin.

Comparison of doxorubicin (DOX), quercetin (QU) and quercetin and doxorubicin (QU-DOX) groups with controls shows that with the presence of quercetin-doxorubicin, it had a greater inhibitory effect on cancer cells.

 

According to research, breast cancer is the second most common cancer among women. Nanomaterials of various materials significantly increase the solubility, stability and effective drug delivery, and in recent years research on the effectiveness of essential oils and plant extracts in inhibiting the growth of cancer cells is expanding. Artemisia belongs to the Asteraceae family. In many studies, the effectiveness of the essential oil and extract of this plant in inhibiting the growth of cancer cells has been reported.

In this experiment, nanoparticles containing Artemisia absinthium L essential oil (thistle) were first synthesized by homogenizer & sonication method and then their physicochemical properties were determined, such as particle size, particle size distribution, zeta potential, percentage of loading efficiency. Then, the anti-apoptotic effect of lipid nanoparticles containing Artemisia essential oil in breast cancer cells (BRC-03) was evaluated.

The results of cellular effect of lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) on breast cancer cells showed that increasing the concentration of lipid nanoparticles containing essential oil of this plant reduced the survival rate of cancer cells. Breast (BRC-03) was purified compared to essential oil (p <0.05).

 Nano-essential oil of this plant is effective in reducing the IC50 of the drug and in increasing the cytotoxicity of the essential oil of the plant. Lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) activated the apoptotic pathway in breast cancer cells.

In late 2019, people around the world became involved with the outbreak of the emerging COVID-19 and its subsequent pandemic. For this reason, researchers in various fields are looking to solve problems related to crisis control and management. The strong transmission power of the SARS-CoV-2 has led experts to use artificial intelligence to combat the pandemic. Artificial intelligence refers to software that, in addition to analyzing data, can have decision-making power, in fact, it is an imitation of expert human intelligence. This study investigates the use of artificial intelligence during the COVID-19 pandemic. Advantages of using artificial intelligence in COVID-19 are presented in the areas of rapid detection and treatment, assistance with rapid diagnosis, monitoring of treatment stages, epidemiology and tracking of infected areas, pandemic status prediction, drug system management, reducing the workload of medical personnel, predicting and monitoring of COVID-19 outbreaks, follow-up of patients, vaccine planning and design. Also, high potential for the application of artificial intelligence in clinical and research laboratories has been estimated. Another potential use of artificial intelligence is to predict mutations that may occur in coronaviruses in the future and cause new symptoms and complications of the disease.

Research has revealed that breast cancer is the second most common cancer among women and that the compounds in Artemisia plant have good anti-tumor potential. Nanomaterials significantly increase solubility, stability, and effective drug delivery. The aim of this study was to evaluate the efficacy of Nano-form of Artemisia essential oil in comparison with non-Nano-form in inhibiting MCF7 cells.

  After extracting essential oil from Artemisia Vulgaris plant, nanoparticles containing Artemisia Vulgaris essential oil were synthesized through homogenizer and sonication method. Different properties of nanoparticles including particle size, zeta potential, and morphology were measured. Finally, the toxicity effect of solid lipid nanoparticles containing essential oil on MCF7 cancer cell line was examined adopting MTT technique.

The results indicated that tDCS significantly reduced the scores on DDQ and OCDUS in the active tDCS group compared to the sham tDCS group (P<.05).

The results of cellular effect of lipid nanoparticles containing Artemisia Vulgaris annua on MCF7 cells showed that increasing the concentration of lipid nanoparticles containing Artemisia Vulgaris essential oil, compared to purified essential oil, reduced the survival rate of MCF7 cells.

The findings show that lipid nanocarriers raise the release rate of essential oil compared to pure essential oil. Increase in concentration of lipid nanoparticles containing Artemisia essential oil reduces the survival rate of breast cancer cells. The IC50 obtained for unbaked essential oils and nanoparticles containing essential oils were 1105 and 262 μg/ml, respectively. Thus, it can be concluded that nanoparticle essential oil of this plant is effective in reducing the IC50 of the drug and increasing cytotoxicity.

The Physalis alkekengi is a herbaceous plant belonging to Solanaceae family. According to research, this plant can help inhibit cancer cell growth.

In this study, the extract of Physalis alkekengi was used to suppress angiogenesis in Balb/c mice. First, the MC4L2 cells were injected in the mice breastbone and the tumor began to grow in this area, then the mice were killed and the tumor removed from the mice and inserted in the test mice by surgical technique. Then, the mice were gavaged for 3 weeks at 50mg/kg and 100mg/kg daily Physalis alkekengi extract, and then the mice were killed and their tumor tissue removed, and tumor growth, apoptotic cell tissue and expression of VEGF gene were studied.

The results of this study showed that the hydroalcholic extract of Physalis alkekengi had a significant effect on tumor growth reduction in experimental groups 2 (50mg/kg body weight) and 3 (100 mg/kg) compared to the control group. Also, the results of the apoptotic cell in tissue showed that the extract of Physalis alkekengi significantly induced apoptotic and necrotic cells in experimental groups in comparison with the control group. The results of RT- PCR showed that the expression of VEGF gene in the experimental groups (50 mg/kg body weight) was significantly (P <0.001) less than that of the control group.

In the end, it was concluded that the extract of the Physalis alkekengi has anti-tumor properties and can be considered as an anti-cancer drug in future clinical studies.

Context:

In late 2019, COVID-19 launched a pandemic from around Wuhan, China. It`s called the SARS-CoV-2 virus which belongs to the corona family and it has a lot in common with SARS-CoV-2, but it has been reported to be more common.

Evidence Acquisition:

The risk of the virus is high for people with high blood pressure and use medication. The reason for this potential and risk for COVID-19 is an increase in expression in a membrane protein called ACE2. This protein is responsible for converting Ang I to Ang1-9 as well as converting Ang II to Ang1-7.

Its pathogenic role is due to its receptor for SARS-CoV-2 and SARS-CoV. Research has shown that there is a significant link between hypertension, Increase the expression and activity of ACE2 and having coronavirus. That`s why our goal is to remind people of high blood pressure about the risk of developing Covid-19. We studied ACE2 and Covid-19 from a clinical and biological point of view. In the following we have shownthe position and the type of virus connection to ACE2 with the help of protein database.

In the SARS-Cov-2, there are four structural proteins and several non-structural proteins together with capsid can contain positive-stranded RNA viruses. Studies have shown that the Spike (S) protein binds strongly to the chain E and F with the ACE2 receptor.

Neurofibromatosis-1(NF-1) is a common genetic condition in children. It is becoming increasingly recognized that in neurofibromatosis, there might be anomalous development of bone with or without any local anomaly of neuro-ectodermal basis. This review was conducted to highlight the different features of spinal disorders in this congenital disease.

Methods and Materials: 

Different search engines were used in this research. After going through the results and discarding the repeated ones, 2 reviewers began to inspect the studies independent of one another. Should any disagreements take place over the inclusion of certain research material into the study, the final decision would belong to the senior writer. In the end, accepted research materials were used as needed in categorizing different types of spinal injuries.

Skeletal defects are among the most impactful complications on the quality of life of patients. Bone deformities, osteoporosis, osteopenia, a reduction of bone mass density, and spinal osteopathy such as scoliosis count among such defects. Although the relative prevalence of spinal defects in neurofibromatosis type 1 is not clear, scoliosis can be considered the most common type of spinal deformity in this illness to the point it is present in 10 to 71% of cases. So, spine surgeons may involve numerous public challenging conditions in NF-1 patients like scoliosis, spondylolisthesis, and dural ectasia.

Thus, Because NF-1 distresses several organ systems, radiologic spine screening is important in patients with NF-1 and patients are likely to profit most from a multidisciplinary treatment policy.

The Secretary-General of the World Health Organization announced a state of emergency on January 30, 2020, to deal with the new coronavirus. The COVID-19 pandemic began in December 2019 in Wuhan, China, and due to the high transmission power of this virus, it quickly involved many problems around the world. Efficient management in some countries minimizes damage. In this article, we examine the management practices of different countries. Quick action, use of experience, forecasting and equipping the required infrastructure, and the use and application of all capabilities seem to be effective factors in controlling the new coronavirus pandemic in some countries.

Aquaporins (AQPs) are water channel proteins. Up to now, 13 AQPs have been known in mammals. AQPs play a key role in water osmotic flow in various cells. the members of aquaporin protein family have been identified as H2O transposters across organelle and plasma membranes. AQPs of the aquaammoniaporin type are highly permeable for water and ammonia. In this study, we have evaluated the structure of AQP5 using two computational methods. We investigated the potential and kinetic energy, as well as maximum and minimum difference of atomic charge for AQP5. The atomic study of AQP5 protein showed that the minimum and maximum value of atomic charge in the presence and absence of water were related to sections (1-15). The water effect is generally considered to be the major driving force in the folding of AQP5. different sections of AQP5 behaved different in the presence or absence of water, and have different functionalities. Also, the absolute value of atomic charge difference for AQP5 sections was proven as an important feature in protein structural changes.HIGHLIGHTS•AQP5’s function and structure are dependent on the presence and absence of water.•The presence of water molecules around AQP5 protein causes changes in dynamic properties.•The optimum functions of AQP5 are arisen at low energy levels and the presence of water.•kinetic energy for AQP5 protein in mode of no water has the lowest fluctuations but in presence of water considerable fluctuations are seen.

Protein-based memory is a novel technology that employs proteins ability to participate in electronic processes. Bacteriorhodopsin (BR) is a membranous proton pump that it’s applications in bio-molecular electronic devices has been widely studied. The results of this research show that BR bounded to modified polycarbonate surface has higher activity for spin coating method.

In an in-vitro study, BR-containing polymer matrix of polyvinyl alcohol and gelatin with different w/v ratios was prepared. Spectroscopic and enzyme activity analysis was performed and the optimized concentration for BR-containing films was determined to be 3.2 mg/ml. BR-polymer was then immobilized on the polycarbonate surface with spin coating method and AFM microscopy was used to characterize BR-coated polycarbonate.

Based on the obtained results we conclude that polymer concentrations below 1% significantly reduced BR activity levels. A280/A570 of 3.64 for 3.2 mg/ml BR solution and 4.97 for BR in 1% polymer confirmed the quality prepared film. AFM study of BR-coated polycarbonate surface revealed the overall thickness of 25nm, indicating that we were able to prepare a surface with suitable thickness for nano electronic devices

The results of this research show that BR bounded to modified polycarbonate surface has higher activity for spin coating method.

Tubulin is known as a heterodimer protein, which includes alpha and beta tubulin subunits. This structural protein plays important roles in pathogenesis and healing different diseases. Biomarkers help in fast and accurate detection of cancer. Proteomic studies can be useful both in biological and clinical research, also help obtain protein expression profiles by using two-dimensional electrophoresis, mass spectrometry, and bioinformatics tools. Finding candidate proteins as cancer biomarkers is an interesting area in proteomic investigations. In the present study, the total protein content of healthy cells of the brain and brain tumor cells were extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, the molecular weight of proteins and protein data banks, which was further confirmed by Matrix Assisted Laser Desorption Ionisation-Time-of-Flight (MALDI-TOF-TOF). In this study, an alpha-tubulin expression found changed (overexpression) in Oligodendroglioma tumors comparing control identified by proteomics analysis. Also, alpha-tubulin position showed in the oligodendroglioma tumors cluster diagram. Proteome analysis approach has allowed biology and medical studies. Alpha-tubulin introduced as a candidate biomarker for the diagnosis and prediction of oligodendroglioma tumors.

Stroke and heart attack are the most common causes of death among humans. Troponin I, Creatine Kinae-MB (CK-MB) and Lactate Dehydrogenase (LDH) are the diagnostic markers of heart attack which can also be used as high risk biomarkers. WiFi is a cheap common technology which exposes its users to a spectrum of electromagnetic waves. Can weak electromagnetic waves affect human health? In this study, stroke in rats has been induced, and then they were exposed to WiFi waves (2.45  GHz) and finally were examined for the risk of heart attack through analyzing three enzyme biomarkers related to heart attack (Troponin I, CK-MB and LDH). This study’s results confirm WiFi’s biological effects and shows WiFi’s contribution in stroke. WiFi2.45GHz exposure affects three cardiac enzyme markers of heart attack (LDH, Troponin I and CK-MB), considering the current data on WiFi exposure effects on the brain, heart and related enzymes. Some of the WiFi wave’s cellular targets include cell membrane, cellular proteins and enzymes. Despite all the data and reports on biological effects of electromagnetic fields, the range and rate of these effects has not yet been determined.

Different disorders and diseases are associated with liver thus liver enzymes are commonly evaluated. Amino transaminases are among the most important enzymes in the liver, which their serum levels can indicate liver’s health or abnormality. Environmental stimuli including electromagnetic field affect different cells and organs in the body including the liver. WiFi networks are among the most common inducers of electromagnetic field. In the present study, serum levels of three liver aminotransaminases including Alkaline phosphatase (ALP), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) are assessed and histopathological evaluations are performed for four weeks in six groups of mice following WiFi2.45GHz exposure. Then, we have analyzed the data (t-test and one-way ANOVA; P

Proteomics is considered a new era in neurophysiological/ neuropathological research including brain tumors. Gliomas which are derived from glial cells are the most common type of brain tumor in humans.
In the present study the total protein content of healthy cells of the brain and brain tumor cells was extracted, purified and quantified by Bradford assay. Two-dimensional electrophoresis were used for protein separation followed by statistical analysis. Primary protein detection was performed based on the differences in isoelectric pH, molecular weight of proteins and protein data banks, which was further confirmed by MALDI-TOF/TOF mass spectrometry (MS).
Our results showed elevated levels of beta-actin protein expression in glioma brain tumor cells. It is important to know when a cell is transformed and when it becomes malignant. Here we evaluated the beta-actin expression in malignant cells.
Since structural changes are highly involved in tumor cell malignancy, beta-actin elevations can contribute in glioma tumor cell invasiveness.

Pseudomonas aeruginosa is considered an opportunistic pathogen; several reports indicate that the organism can also cause infections in healthy hosts. Four effector proteins have been described in P. aeruginosa: exoU, exoS, exoT, and exoY. These genes that are translated into protein products related to type III secretion systems.
A total of 134 samples were isolated, and P. aeruginosa was identified using biochemical tests. Bacterial genomic DNA was extracted, and the presence of the exoSand exoUgenes were detected by PCR. Biofilms were formed by culturing P. aeruginosaon glass slides in rich medium.
The exoU(73%), exoS (62%) genes were detected from infections caused by P. aeruginosa in urinary tract infection patients. Among the 119 strains isolated from patients with urinary tract infections.
An improved understanding of virulence genes and biofilm formation in P.aeruginosa may facilitate the future development of novel vaccines and drug treatments.

Gliomas are the most frequently observed primary brain tumors. These tumors comprise a variety of different histological tumor types and malignancy grades. Oligodendrogliomas typically contain a rich network of branching capillaries. Approximately 50%-80% of oligodendrogliomas demonstrate a combined loss of chromosomes 1p and 19q. Oligodendrogliomas differ from neurocytomas in that they show a diffusely infiltrating pattern of spread that precludes surgical cure. We evaluated extracted proteins from tumors and normal brain tissues for protein purity by the Bradford test and spectrophotometry. We separated proteins by two-dimensional gel electrophoresis. The spots were analyzed and compared using statistical data and MALDI-TOF/TOF. Protein clustering analyses were performed on the list of proteins deemed significantly altered in oligodendroglioma tumor tissues. On each analytical two-dimensional gel, we observed an average of 1328 spots. A total of 157 exhibited up-regulation of expression levels, whereas the remaining 276 spots had decreased expression in astrocytoma tumors relative to normal tissue. The results demonstrated that functional clustering and principal component analysis had considerable merit in aiding the interpretation of proteomic data. Clustering methodology is a powerful data mining approach for initial exploration of proteomic data. The clustering results depend on parameters such as data preprocessing, between-profile similarity measurement and the dendrogram construction procedure.

Male infertility is a distressingly common condition affecting about 1 in 20 of the male population. In a majority of the cases, the male partner produces sufficient numbers of spermatozoa to achieve fertilization but there are functional defects in these cells that prevent conception from occurring. In this study, the effects of Wi-Fi radiation on spermatogenesis, testes and testosterone of large white male rats were studied, and also hydro alcoholic extract of sea buckthorn fruit were used as a protective agent against the effects of Wi-Fi radiation, and the comparative study was done.For this study 60 white male laboratory rats weighing 200-250 g were selected then tested in good conditions of light and food. The mice were randomly divided in terms of Wi-Fi Radiation and received gavage Sea buckthorn into 6 out of groups of 10.It was found that in the groups that had received Wi-Fi radiation, waves did not affect sperm count, testes weight, testosterone and testicular tissue, but it caused a significant decrease in fertilized sperm motility, and sea buckthorn extract could work as a protective agent against the radiations. According to the results of this research, it can be the probability that Sea buckthorn extract, having antioxidant substance can prevent destructive effects of waves that cause free radicals and having vitamin E improves sperm quality.

EGFR is a key molecule in cancer cells. EGFR signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. We extracted proteins from astrocytoma (III and IV) oligodendroglioma(IV) tumors and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometry method. In this study, we separated proteins by the two-dimensional gel (2DG) electrophoresis method, and the spots were analyzed and compared using statistical data and Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-TOF MS). EGFR position showed in the diagram cluster for oligodendroglioma tumors (t-test and one-way ANOVA; P

Nowadays brain tumors remain as a significant cause of morbidity and mortality and are often treatment refractory. The grading of brain tumor has an important implication in clinical management. Currently, magnetic resonance spectroscopy (MRS) is an important modality in evaluating and grading brain tumors. The aim of this study is evaluation of metabolites in the differentiation of brain tumors and grading of brain gliomas using HMRS (proton magnetic resonance spectroscopy). The studies were performed using single voxel MRS، 3Tesla with pulse of sequence used for MRS was point resolved single volume spectroscopy (PRESS) with repetition time (TR) 1000-6000ms and echo time (TE) 36-136ms and The detected distinguished metabolites was included Choline (Cho), Creatin (Cr), and N-acetyl a aspartate (NAA), 37cases had data which passed quality control. The Patient ages ranged from 7 To 81 years, 17 were male and 20 female. MRS data was processed using SYNGO software to give mean spectra and metabolite concentrations which were compared using min it a band SPSS. To test the primary hypothesis, univariate logistic regression was performed on each individual measured metabolite quantity. Significant differences were found in concentrations of key metabolites and Cho/NAA and Cho/Cr ratios using T-test and significance (P≤0. 05). In the assessment of age rate and tumor grading, the malignancies of brain tumors did not correlate with patients’ ages as well as sexuality. MRS can detect subtle differences between low grade brain tumors in children and should form part of the clinical assessment of these tumors.

The bacterium Pseudomonas aeruginosa is a common cause of infections, and infection with antibiotic-resistant strains can be dangerous. The aim of the present study is to investigate the presence of the exoS and exoU genes and the formation of biofilms in clinically isolated P.aeruginosa strains. A total of 126 samples were isolated, and P.aeruginosa was identified using biochemical tests. Bacterial genomic DNA was extracted, and the presence of the exoS and exoU genes were detected by PCR. Biofilms were formed by culturing P.aeruginosaon glass slides in rich medium. The exoU (76%) exoS (68%) genes were detected in of strains isolated from burn (burns caused by heat) patients, respectively. Among the 113 strains isolated from patients with burn infections. An improved understanding of virulence genes and biofilm formation in P.aeruginosa may facilitate the future development of novel vaccines and drug treatments.

Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding. The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks. The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values. This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.

Glioblastoma multiforme (GBM) or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. Several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications, proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. We extracted proteins of tumor and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometery method. In this study, we separated proteins by two-dimensional (2DG) gel electrophoresis method and the spots were then analyzed and compared using statistical data and specific software. Proteins clustering analyses were performed on the list of proteins deemed significantly altered in glioblastoma tumors (t-test and one-way ANOVA; P < 0.05). The 2D gel showed totally 876 spots. We reported, 172 spots exhibited different in expression level (fold > 2) for glioblastoma. On each analytical 2D gel, an average of 876 spots was observed. In this study, 188 spots exhibited up regulation of expression level, whereas the remaining 232 spots decreased in glioblastoma tumor relative to normal tissue. Results demonstrate that functional clustering (up and down regulated) and principal component analysis has considerable merits in aiding the interpretation of proteomic data. At the core of proteomics was 2D gel electrophoresis witch permitted the separation of thousands of protein, High resolution 2DE can resolve up to 5,000 proteins simultaneously. Using cluster analysis, we can also from groups of related variables, similar to what you do in factor analysis.

Protein memories are novel technologies which use the hidden abilities of bacteria proteins and biological molecules in order to the electronic processes. Four decades before baceriorhdopsin (BR) protein was extracted from the membrane of Halobacerinum Salina rum found in high salt water depositions after its original discovery in 1967. In this study, for immobilizing the protein a film has been provided from the different weight/volume use of polyvinyl alcohol and gelatin polymers. Then a suspension of protein bacteriorhodopsin with the 3/2 mg.ml -1 has been used that according to the spectroscopy and determining their activity, optimizing concentration of a film containing bacteriodospian has been obtained. Polymeric film containing bacteriorhodopsin protein has been injected on the glass and by use of spin coater, after obtaining optimizing protocol become stabilized. After that the biological activity of the bacteriorhodopsin protein has been studied. Several biological molecules are being considered based on their usage in computers sciences, as though the bacterial protein – BR has generated much interest among scientists. Functional immobilization of membrane proteins requires consideration of their physiological needs, often dictated by the quality and components of the natural hydrophobic environment surrounding this class of proteins.

Asterocytoma is the most common primary human brain tumor which has the most lethal hazard among tumors in human Central Nervous System. Heat Shock Proteins play an active role in cancer cells pathways through their effects on tumor cell proliferation, tumor differentiation, malignancy, metastasis, and cell death. HSP70 is a 70 KD protein that its expression change has been reported in various kinds of cancers. Here a proteomic activity for molecular diagnosis of Asterocytomas tumors is designed. Proteins of tumor and normal brain tissues were extracted and then evaluated by Bradford test. In this study, the proteins were separated by 2DG Electrophoresis method, and then the spots were analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by Isoelectric point (pI), molecular weights and data banks. As a result, the 2D gel showed totally 800 spots. HSP70 is one of down regulated proteins. As conclusion, alteration of HSP70 expression was detected in Asterocytomas which has an important role in tumor survival and development, response to cell stress and induces apoptosis in cells. Three kinds of HSP70 are known in different cancers: HSP70-1, HSP70-2 and HSP70-8 which affect limitation of apoptosis system in cancer cells.