mehrdad farsi

Tubal factor is a very Common cause of infertility. This study was performed to assess the therapeutic effect of Intrauterine Insemination (IUI) in patients with unilateral fallopian tube obstruction and to reduce indications of IVF and ICSI. This case-control study was performed between years 2008-2009 in Fatemeh-Zahra infertility center of Babol on 60 patients with unilateral tube occlusion (case group) and one 120 infertile patients without tubal obstruction (control group) in HSG. Subjects were selected by sampling method. Demographic data were collected by a researcher-made questionnaire and analyzed by SPSS Software (version 16) using descriptive statistical methods and t-test and Chi-square test. Pregnancy was achieved in 4 patients (6.7%) of case group and 15 patients (12.5%) in control group but the difference was not significant (P>0.05). All 4 pregnancy cases had left ovarian tube obstruction and none of patients with right ovarian tube obstruction became pregnant, but the difference was not significant (P>0.05). Pregnancy rate had no significant difference base on site (distal or proximal) of involvement (p=0.99). Pregnancy rate after IUI has no significant difference in patients with unilateral tube obstruction and infertile women with patent ovarian tubes. Therefore, IUI can be suggested to patients with unilateral ovarian tube obstruction regardless of involvement site.

This study aimed to evaluate the effect of seasonal variability on the assisted reproductive technique (ART) success rate. This study was a descriptive – analytical survey performed on 91 infertile women undergoing intracytoplasmic sperm injection – embryo transfer (ICSI-ET) in different seasons. The patients aged less than 35 years old and had normal LH/FSH ratio. All patients entered long protocol down regulation treatment cycle and the picked up oocytes were transferred to GIII medium in the infertility laboratory. The cumulus characteristics, oocyte parameters including number of the retrieved oocytes, morphological characteristics, fertilization and degeneration rate and number of cleaved embryos were recorded. Data were analyzed by SPSS software. The number of embryos was significantly higher in autumn. Abnormal morphological parameters (color, size, zona thickness) and the degeneration rate were significantly higher in spring. The number of retrieved oocytes, MI, MII oocytes and fertilization rate had no significant seasonal variations. The results of this study showed a significant seasonal variation in morphological parameters of the oocytes, degeneration rate and the number of formed embryos.

There are different methods to improve sperm parmeters including swim up.Sperm washing is a basic step in This method during 2007. It seems that two times washing cause bether result especially in high viscosity sample and sample it is not liqufy. The aim of this study is to compare the single and double washing swim up method on sperm parameters. This study was carried out on 50 semen samples referred to Fatemeh-Alzahra infertility center of Babol University of Medical Sciences during 2007. Semen samples had the following characteristics: volume 2-5ml, count>20 million/ml, motility>50% and normal morphology>14%. Sampling condition were completely considered. Sperm parameter analysis carried out after two times washing. Data entered to SPSS-10 and analyzed by repeated measurement and paired T-Test and p-value<0.05 considered statistically significant. Semen volume mean was 4.050±1.006. Mean sperm motility in one and two times washings increased (P<0.05), but there was not statistically significant differences between one and two times washing. In grading analysis, by increasing the washing, grade III, IV increase and grade I decrease (P<0.05). Sperm morphology mean increased with washing but it is not statistically different between one and two times washing. This study showed that swim up method is effective method for sperm preparation and two times washing cause recuperation of sperm parameters.

One of the causes of infertility is due to male factors (Sperm count, sperm motility and morphology). Male factor infertility may be due to disorders in the number of spermatozoa, sperm motility or sperm morphology. Intrauterine insemination (IUI) is routinely used to help couples with male factor infertility. The criteria used to evaluate semen quality include sperm concentration, sperm motility and the percentage of sperm with normal morpho-logy. Several studies have been done on motility and sperm concentration but few studies have been done on sperm morphology. In this study, we evaluated IUI success rates in normal sperm morphologies that were greater or fewer than 5% in the Northern part of Iran (Mazandaran). This analytical study was performed on patients referred to two inferti-lity centers in Babol after ovulation induction. After collecting sperm specimens from the cases, the samples were kept in sterile tubes and then they were washed and swum up before perform-ing IUIs. Sperm concentration, motility and morphology were evaluated according to NAFA and ESHRE-SIGA criteria (2002). Statistical analysis was done by SPSS software using logistic regression, paired t-tests and Fisher’s exact test. A p–value < 0.05 was considered significant. Out of 561 patients, IUIs were successful in 103 (20.2%) patients with normal sperm morphology greater than 5% and in 4 (7.7%) patients with normal sperm morphology fewer than 5%; the difference being statistically significant (p<0.05). In 504 cases with sperm concen-trations greater than 20 million/ml, IUI success rates in subjects with normal sperm morphology greater than 5 % were significantly higher than cases with normal sperm morphology fewer than 5% (21.4:5.6), (p<0.01). In 543 cases with sperm motility greater than 50%, IUI success rates were significantly higher in patients with normal sperm morphology greater than 5% compared to the cases with normal sperm morphology fewer than 5% (20.5:8.3), (p<0.05). It seems that normal sperm morphology >5% is an important and effective factor in IUI outcomes and normal sperm morphology, alongside sperm counts and normal motility, has an essential role in increasing pregnancy rates. Therefore, it is suggested that in addition to sperm counts and motility, evaluations of sperm morphology be included in the work up too.