فهرست مطالب mohamadreza mehrabi

Tissue engineering, by combining different fields such as physics, nanotechnology, molecular biology and evolution, on the one hand, tries to provide a more complete and better understanding of cellular processes, and on the other hand, to improve engineering methods and tissue production. Alternatives for medical use. Nanotechnology, meanwhile, has led to significant advances in the development of new materials for use in the biomedical field. Nanomaterials with the aim of facilitating the creation of tissue engineering structures have the ability to be designed to create the least inflammatory response in the host body. Therefore, knowledge of the impact of these properties on cell behavior is essential, both in early cell research and in application-related areas, such as tissue engineering and cell-based technologies. Biomaterials with a high surface-to-volume ratio contribute significantly to the adsorption capacity and chemical activity, which ultimately leads to increased cellular function. Also, biological nanomaterials, compared to conventional conventional biomaterials, have a high potential for loading drugs and nucleic acids or other biological factors to accelerate tissue repair and regeneration. This paper reviews the applications of biomaterials in tissue engineering and regenerative medicine and discusses the role of these materials as additives in tissue engineering scaffolds as well as induction factors in the culture medium.

Helicobacter pylori (H. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. The 16s rRNA is a common H. pylori gene which are usually preferred for diagnosis purpose. The aim of this study was to determine the prevalence and abundance of 16s rRNA in fecal samples and also evaluate correlation between the level of 16s rRNA and activities of the cytokines, TNF-&alpha and IL-1&beta, in serum.

The present study was performed on 84 subjects with digestion problems. Fecal and blood samples were collected and 16s rRNA gene was assayed using PCR. The serum levels of TNF-&alpha and IL-1&beta levels were measured by enzyme-linked immunosorbent assay (ELISA).

The results of the study revealed that there was a positive correlation between the 16s rRNA gene, H. pylori stool antigen (HPSA) and TNF-&alpha cytokine. The study also noted that with every unit of increase in either of the quantified parameters of HPSA and IL-1&beta, the presence of 16s rRNA in fecal samples, showed a 2.98 and 1.01 times rise, respectively.

According to the obtained results, it may be concluded that activities of cytokine TNF-a correlated well with the presence of HPSA and 16srRNA gene in the stomach’s lining. Increase in the activities of HPSA and TNF-a cytokine could be associated with the presence of 16s rRNA in feces.