mohammad alahtavakoli

Aging process is inevitable, which increases the risk of illness and death in people. Menopause occurs as a part of the aging process. The aim of this study was to evaluate the effect of metformin (Met) on muscle strength, anxiety and memory in ovariectomized mice Thiswas an experimental study. 96 female mice (Age 6 months) (20-25 g) were randomly divided into sex groups, including: (1) sham, (2) ovariectomized, (3 and 4) ovariectomized + Met (1 and 10 mg/kg) (5) Met 10 mg/kg, and (6) healthy. At first, the mice were ovariectomized and then received Met for six months. Muscle strength, memory, and anxiety in the exprimental groups was examined. Statistical analysis was performed via SPSS16 and the differences between groups were analyzed by one way ANOVA. The results of the study showed Met with doses of 1 mg / kg and 10 mg significantly reduced the anxiety of mice (p = 0.0295 and p = 0.0024, respectively), increased memory (p = 0.0004 and p = 0.0044, respectively) and at dose of 10 mg / kg it improved muscle strength (p = 0.0148) compared to the ovariectomy group. Metformin has beneficial effects on behavioural manifestations of aging and ovarectomy

Background and Both hypothermia and Decompressive craniectomy (DC) have been shown to reduce ischemic injury in experimental middle cerebral artery occlusion (MCAO). This study was designed to evaluate the effect of delayed combination of DC and Local Hypothermia (LH) on infarct size, neurological outcome and blood brain barrier (BBB) disruption in a rat model of stroke. MCAO was induced in 36 Wistar rats assigned to three groups of control, Decompressive Craniectomy (DC) and combination of Hypothermia and Craniectomy (HC). Infarct size and BBB disruption were measured 48 hour after ischemia insult. Neurological deficits were assessed at 24 and 48 hours after stroke using sticky tape test, hanging-wire test and Bederson’s scoring system. BBB disruption was measured by Evans blue dye leakage. Compared to the control group, infarct size reduced significantly in DC and HC groups (P<0.001), however, combination therapy was more neuroprotective compared to craniectomy alone (P<0.01). Compared to the control group, BBB disruption was significantly reduced in DC (P<0.05) and HC (P<0.01).While compared to the control group sticky tape test (P<0.05 at 24 h; P<0.001 at 48 h) and hanging-wire test (P<0.05) showed better behavioral performance only in HC, and Bederson test showed improved behavioral functions of HC animals (P<0.01). At the same time neurological deficits also decreased in HC group (P<0.05 at 24 h; P<0.01 at 48 h) compared to the DC group. According to this study although both delayed local hypothermia and craniectomy are protective after stoke, combination therapy of them is more neuroprotective than given alone.

Plants Antioxidants are of good candidates for protection against the toxicity of industrial pollution. In this study، the protective effect of hydro alcoholic extract of Teucrium polium (TP) on Mercuric chloride-induced nephrotoxicity was assessed.

In this experimental study 28 male wistar rats were examined. Animals were randomly divided into 4 groups (n=7) as: Control، Mercuric chloride، Mercuric chloride with extract of TP and extract of TP. Mercuric chloride and TP extracts were intraperitoneally injected to the with the dose of 1. 5 mg/kg and 200 mg/kg each day for 8 days، respectively. Then، blood samples were prepared and nephrotoxicity was determined by measuring serum urea and creatinine، using spectrophotometry and specific kit. One-way ANOVA followed by Tukey test was used for data analysis in SPSS software.

Mercuric chloride caused a significant increase in serum urea and creatinine in the Mercuric chloride group compared to the control group (P<0. 05). TP extract caused a significant decrease in serum urea and creatinine in the combined TP extract with Mercuric chloride group compared with Mercuric chloride group (P<0. 001).

Our results showed that the extract of TP has protective effects against nephrotoxicity induced by heavy metals such as mercury.