mohammad chaichi

Two copper (II) complexes of Quercetin (Q) with metal:ligand stoichiometry ratios of 1:1 and 1:2 were synthesized. The synthesized complexes structures were determined by UV-Vis spectroscopy, Infra-red spectroscopy, thermogravimetry, elemental analyses as well as cyclic voltammetry. The spectroscopic data suggest that the chelation between Q and Copper (II) can occur through a 3-hydroxy-4-carbonyl site. The antioxidant effectiveness and scavenging activity of quercetin and its complexes were evaluated using DPPH method and luminol-hydrogen peroxide chemiluminescence system. The results showed that prepared compounds have strong quenching effects on the chemiluminescence intensity of luminol in the alkaline medium and are efficient in scavenging free radicals produced in this system. Reaction system variables such as luminol, quercetin and their complexes' concentration and various pH of luminol solution were investigated. Stren-Volmer equation was also used to study the kinetics of the luminol reaction system and linear relationship was obtained between CL intensity and the concentration of these compounds in a range of 10-100 mg/L. The Stern–Volmer quenching constant, Kq value in the presence of free quercetin is higher than the complexes, which indicates the fact that complexation with metal ions changes the chemical properties of quercetin and decreases the inhibitory ability and radical scavenging efficiency. The concentration-dependent inhibitory ability of quercetin and copper complexes is attributable to the presence of several hydroxyl groups in their structure that react with superoxide or peroxyl radicals available in luminol-H2O2 system. Our study strongly confirmed the potent radical scavenging property of polyphenolic compounds and their metal complexes.

Pseudomonas aeruginosa is a gram negative bacillus. Infection with these bacteria usually occurs in patients who hospitalized due to: need for prolonged stay in ICU, those who have intravascular catheters, or indwelling urinary catheters. This Study investigates in vitro susceptibility of pseudomonas aeruginosa which were isolated from patients with UTI to doxycycline, tigecycline, ceftazidime, cefepime, piperacillin/tazobactam, impipenem, meropenem, colistin, ciprofloxacin, nitrofurantoin, amikacin) with standard disk diffusion. Comparing the, susceptibility results of disk diffusion with E.test for doxycycline and tigecycline is another aim. Other purpose of this study was to define MDR pseudomonas aeruginosa susceptibility to doxycycline and tigecycline .

Sixty pseudomonas aeruginosa which was isolated after biochemical and bacteriological test, were entered to study and susceptibility to above antibiotics was tested by disk diffusion. For doxycycline and tigecycline antibiogram by two methods were performed: disk diffusion and Etest.

Maximum susceptibility was observed for colistin =95% followed by sensitivity in decreasing order for these antibiotics .Meropenem 76% Imipenem= Cefepime= Ceftazidime 63.3% Piperacillin/tazobactam 55% doxycycline 46% amikacin 45% Ciprofloxacine 35% Tigeycline 15% nitrofurantoin 0%. Measurement of kappa for agreement of two methods of antibiograms revealed moderate agreement in this regard.

In vitro susceptibility of isolated pseudomonas aeruginosa to studied antibiotics (with exception of colistin) was less than 80% even for carbapenems. Disk diffusion for doxycycline and tigecycline wasn’t effective for differentiation intermediate resistance from sensitive. Calculation of cohens Kappa for agreement was moderate. Most of MDR pseudomonas aeruginosa was resistant to doxycycline and tigecycline.

Myiasis is caused by the larvae of flies infesting animal or human tissues and organs. This report aims to present a case of pharyngeal myiasis caused by the larvae of Oestrus ovis (Diptera: Oestridae). A 55-yr old drug addict living in the Shahindeje village of Western Azerbaijan Province, northwestern Iran was referred to the Emam Reza Hospi­tal in Tabriz, having a medical history of Chronic Obstructive Pulmonary Disease (COPD) and hospitalized due to respiratory distress, 20 days ago. He was intubated with a mechanical ventilator (MV) because of his respiratory distress condition. There was an evidence of the presence of pulmonary nodules in his lungs following diagnosis, and a CT scan revealed a cavity in his lung. During the nasogastric intubation procedure, a larva was seen emerging from the patient’s mouth by one of the staff of the intensive care unit of the hospital. A laboratory diagnosis was per­formed in the Entomology Department of the School of Medicine, Tabriz University of Medical Sciences. Interest­ingly, larvae of O. ovis were identified and confirmed following the laboratory proceedings.

Eriobotrya japonica belongs to the Rosaceae. Studies have shown that the flowers of this plant are rich in phenolic and flavonoid compounds. Accorrdingly, the evaluation of antioxidative effects of Eriobotrya japonica Flower Extract (EJFE) have been performed in vitro. In this study, to investigate the influences of components of EJFE on its antioxidative activity, extract was prepared using hydro-alcoholic (25:75 V/V) solvent and the antioxidative activity of the extract was evaluated based on the scavenging of various radicals (DPPH and H2O2) by spectrophotometric method and chelating of ferrous ions by ferrozine reagent. HPLC analysis of the Eriobotrya japonica Flower Extract (EJFE) revealed hesperetin and gallic acid as the major antioxidants. When the content of total flavonoid and polyphenolic compounds in the flower extract of this plant was examined, a significantly higher level of total polyphenols was found in Eriobotrya japonica flower extract. Results demonstrate that the high ability to scavenge free radicals, reducing power, and Fe+2chelating activity exerted by the EJFE were due to the high content of hesperetin and gallic acid in the flowers.